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Am J Pathol. 1988 Apr; 131(1): 19–28.
PMCID: PMC1880579

Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins.


The authors have recently developed a monoclonal antibody, HHF35, that recognizes the muscle-specific isoforms of actin. To determine its potential usefulness in the differential diagnosis of "small, round, blue cell" tumors of childhood, they immunolabeled formalinor B-5-fixed tissue sections from known cases of rhabdomyosarcoma or rhabdomyoma (30), neuroblastoma (9), retinoblastoma (2), and Ewing's sarcoma (9) with HHF35 and with antibodies to creatine kinase M, myoglobin, vimentin, and neuron-specific enolase. HHF35 reacted with 29 of 30 cases of rhabdomyosarcoma, whereas antibodies to creatine kinase M and myoglobin were positive on only 12 and 7 tumors, respectively. HHF35 did not react with any case of neuroblastoma, retinoblastoma, or Ewing's sarcoma when the antibody diluent contained 50 mM EDTA. These results indicate that HHF35 is a highly sensitive and specific marker for myogenic differentiation and that it will be useful in the differential diagnosis of rhabdomyosarcomas.

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Selected References

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  • Darbyshire PJ, Bourne SP, Allan PM, Berry J, Oakhill A, Kemshead JT, Coakham HB. The use of a panel of monoclonal antibodies in pediatric oncology. Cancer. 1987 Feb 15;59(4):726–730. [PubMed]
  • de Jong AS, van Kessel-van Vark M, Albus-Lutter CE, Voûte PA. Creatine kinase subunits M and B as markers in the diagnosis of poorly differentiated rhabdomyosarcomas in children. Hum Pathol. 1985 Sep;16(9):924–928. [PubMed]
  • Kahn HJ, Yeger H, Kassim O, Jorgensen AO, MacLennan DH, Baumal R, Smith CR, Phillips MJ. Immunohistochemical and electron microscopic assessment of childhood rhabdomyosarcoma. Increased frequency of diagnosis over routine histologic methods. Cancer. 1983 May 15;51(10):1897–1903. [PubMed]
  • Scupham R, Gilbert EF, Wilde J, Wiedrich TA. Immunohistochemical studies of rhabdomyosarcoma. Arch Pathol Lab Med. 1986 Sep;110(9):818–821. [PubMed]
  • Altmannsberger M, Weber K, Droste R, Osborn M. Desmin is a specific marker for rhabdomyosarcomas of human and rat origin. Am J Pathol. 1985 Jan;118(1):85–95. [PMC free article] [PubMed]
  • Jong AS, van Vark M, Albus-Lutter CE, van Raamsdonk W, Voûte PA. Myosin and myoglobin as tumor markers in the diagnosis of rhabdomyosarcoma. A comparative study. Am J Surg Pathol. 1984 Jul;8(7):521–528. [PubMed]
  • de Jong AS, van Kessel-van Vark M, Albus-Lutter CE, van Raamsdonk W, Voûte PA. Skeletal muscle actin as tumor marker in the diagnosis of rhabdomyosarcoma in childhood. Am J Surg Pathol. 1985 Jul;9(7):467–474. [PubMed]
  • Molenaar WM, Oosterhuis JW, Oosterhuis AM, Ramaekers FC. Mesenchymal and muscle-specific intermediate filaments (vimentin and desmin) in relation to differentiation in childhood rhabdomyosarcomas. Hum Pathol. 1985 Aug;16(8):838–843. [PubMed]
  • Mukai M, Iri H, Torikata C, Kageyama K, Morikawa Y, Shimizu K. Immunoperoxidase demonstration of a new muscle protein (Z-protein) in myogenic tumors as a diagnostic aid. Am J Pathol. 1984 Jan;114(1):164–170. [PMC free article] [PubMed]
  • Osborn M, Hill C, Altmannsberger M, Weber K. Monoclonal antibodies to titin in conjunction with antibodies to desmin separate rhabdomyosarcomas from other tumor types. Lab Invest. 1986 Jul;55(1):101–108. [PubMed]
  • Royds JA, Variend S, Timperley WR, Taylor CB. Comparison of beta enolase and myoglobin as histological markers of rhabdomyosarcoma. J Clin Pathol. 1985 Nov;38(11):1258–1260. [PMC free article] [PubMed]
  • Schiaffino S, Gorza L, Sartore S, Saggin L, Carli M. Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study. Exp Cell Res. 1986 Mar;163(1):211–220. [PubMed]
  • Tsukada T, Tippens D, Gordon D, Ross R, Gown AM. HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol. 1987 Jan;126(1):51–60. [PMC free article] [PubMed]
  • Tsukada T, McNutt MA, Ross R, Gown AM. HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues. Am J Pathol. 1987 May;127(2):389–402. [PMC free article] [PubMed]
  • Garrels JI, Gibson W. Identification and characterization of multiple forms of actin. Cell. 1976 Dec;9(4 Pt 2):793–805. [PubMed]
  • Gonazlez-Crussi F, Black-Schaffer S. Rhabdomyosarcoma of infancy and childhood. Problems of morphologic classification. Am J Surg Pathol. 1979 Apr;3(2):157–171. [PubMed]
  • Brooks JJ. Immunohistochemistry of soft tissue tumors. Myoglobin as a tumor marker for rhabdomyosarcoma. Cancer. 1982 Nov 1;50(9):1757–1763. [PubMed]
  • Eusebi V, Bondi A, Rosai J. Immunohistochemical localization of myoglobin in nonmuscular cells. Am J Surg Pathol. 1984 Jan;8(1):51–55. [PubMed]
  • Altmannsberger M, Osborn M, Treuner J, Hölscher A, Weber K, Shauer A. Diagnosis of human childhood rhabdomyosarcoma of antibodies to desmin, the structural protein of muscle specific intermediate filaments. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982;39(2):203–215. [PubMed]
  • Miettinen M, Lehto VP, Badley RA, Virtanen I. Alveolar rhabdomyosarcoma. Demonstration of the muscle type of intermediate filament protein, desmin, as a diagnostic aid. Am J Pathol. 1982 Aug;108(2):246–251. [PMC free article] [PubMed]
  • Yates LD, Greaser ML. Troponin subunit stoichiometry and content in rabbit skeletal muscle and myofibrils. J Biol Chem. 1983 May 10;258(9):5770–5774. [PubMed]
  • Potter JD. The content of troponin, tropomyosin, actin, and myosin in rabbit skeletal muscle myofibrils. Arch Biochem Biophys. 1974 Jun;162(2):436–441. [PubMed]
  • Wang K. Sarcomere-associated cytoskeletal lattices in striated muscle. Review and hypothesis. Cell Muscle Motil. 1985;6:315–369. [PubMed]
  • Jansson E, Sylvén C, Nordevang E. Myoglobin in the quadriceps femoris muscle of competitive cyclists and untrained men. Acta Physiol Scand. 1982 Apr;114(4):627–629. [PubMed]
  • Bandman E, Matsuda R, Strohman RC. Developmental appearance of myosin heavy and light chain isoforms in vivo and in vitro in chicken skeletal muscle. Dev Biol. 1982 Oct;93(2):508–518. [PubMed]
  • Daubas P, Caput D, Buckingham M, Gros F. A comparison between the synthesis of contractile proteins and the accumulation of their translatable mRNAs during calf myoblast differentiation. Dev Biol. 1981 May;84(1):133–143. [PubMed]
  • Devlin RB, Emerson CP., Jr Coordinate regulation of contractile protein synthesis during myoblast differentiation. Cell. 1978 Apr;13(4):599–611. [PubMed]
  • Heywood SM, Thibault MC, Siegel E. Control of gene expression in muscle development. Cell Muscle Motil. 1983;3:157–193. [PubMed]
  • Shani M, Zevin-Sonkin D, Saxel O, Carmon Y, Katcoff D, Nudel U, Yaffe D. The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of corresponding mRNA sequences during myogenesis. Dev Biol. 1981 Sep;86(2):483–492. [PubMed]

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