• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of amjpatholAmerican Journal of Pathology For AuthorsAmerican Journal of Pathology SubscribeAmerican Journal of Pathology SearchAmerican Journal of Pathology Current IssueAmerican Journal of Pathology About the JournalAmerican Journal of Pathology
Am J Pathol. Sep 1990; 137(3): 593–603.
PMCID: PMC1877516

Beta microseminoprotein is not a prostate-specific protein. Its identification in mucous glands and secretions.


Beta microseminoprotein (beta inhibin, PSP94), an unglycosylated protein of 94 amino acids with unknown function, is one of the predominating proteins in the secretion of the human prostate gland. In this work the authors have demonstrated that the expression of beta microseminoprotein is not restricted to the prostate and that the protein has a previously unrecognized widespread occurrence in the human body. According to radioimmunoassay, beta microseminoprotein immunoreactivity is present in many nonprostatic body fluids. The highest concentrations were found in secretions from the respiratory tract; in tracheobronchial fluid sometimes even at concentrations comparable to that in seminal plasma (about 1 g/l). Intermediate concentrations were found in gastric juice and some samples of secretion from the uterine cervix, whereas tears, saliva, pancreatic juice, bile, and mucus from the colon had low concentrations. According to gel chromatography, the molecular size of the beta microseminoprotein immunoreactivity present in tracheal fluid, gastric juice, and secretion from the uterine cervix did not differ from that of beta microseminoprotein in seminal plasma. The beta microseminoprotein immunoreactive component present in gastric juice had the same amino-terminal amino acid sequence as prostatic beta microseminoprotein (14 residues identified in material purified from gastric juice), providing further evidence for chemical identity of a nonprostatic beta microseminoprotein with the prostatic protein. Immunohistochemical staining with affinity-purified antibodies demonstrated the presence of beta microseminoprotein in many tissues, including the goblet cells in the tracheobronchial epithelium, tracheobronchial submucosal glands, certain mucosal cells in the antrum of the stomach, some glands of Brunner in the duodenum, and in parts of the mucosa of the colon. At least in the respiratory tract, the staining was localized to mucus-containing cells. beta microseminoprotein immunoreactivity also was localized to the cilia of the ciliated epithelium in the respiratory tract, the fallopian tubes, and the Gartner ducts of the uterine cervix. The pattern of tissue distribution of beta microseminoprotein found in this work indicates a connection of beta microseminoprotein with mucous secretions.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.8M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Seidah NG, Arbatti NJ, Rochemont J, Sheth AR, Chrétien M. Complete amino acid sequence of human seminal plasma beta-inhibin. Prediction of post Gln-Arg cleavage as a maturation site. FEBS Lett. 1984 Oct 1;175(2):349–355. [PubMed]
  • Johansson J, Sheth A, Cederlund E, Jörnvall H. Analysis of an inhibin preparation reveals apparent identity between a peptide with inhibin-like activity and a sperm-coating antigen. FEBS Lett. 1984 Oct 15;176(1):21–26. [PubMed]
  • Akiyama K, Yoshioka Y, Schmid K, Offner GD, Troxler RF, Tsuda R, Hara M. The amino acid sequence of human beta-microseminoprotein. Biochim Biophys Acta. 1985 Jun 10;829(2):288–294. [PubMed]
  • Mbikay M, Nolet S, Fournier S, Benjannet S, Chapdelaine P, Paradis G, Dubé JY, Tremblay R, Lazure C, Seidah NG, et al. Molecular cloning and sequence of the cDNA for a 94-amino-acid seminal plasma protein secreted by the human prostate. DNA. 1987 Feb;6(1):23–29. [PubMed]
  • Dubé JY, Frenette G, Paquin R, Chapdelaine P, Tremblay J, Tremblay RR, Lazure C, Seidah N, Chrétien M. Isolation from human seminal plasma of an abundant 16-kDa protein originating from the prostate, its identification with a 94-residue peptide originally described as beta-inhibin. J Androl. 1987 May-Jun;8(3):182–189. [PubMed]
  • Lilja H, Abrahamsson PA. Three predominant proteins secreted by the human prostate gland. Prostate. 1988;12(1):29–38. [PubMed]
  • Abrahamsson PA, Andersson C, Björk T, Fernlund P, Lilja H, Murne A, Weiber H. Radioimmunoassay of beta-microseminoprotein, a prostatic-secreted protein present in sera of both men and women. Clin Chem. 1989 Jul;35(7):1497–1503. [PubMed]
  • Thakur AN, Vaze AY, Dattatreyamurty B, Arbatti NJ, Sheth AR. Isolation & characterization of inhibin from human seminal plasma. Indian J Exp Biol. 1978 Jul;16(7):854–856. [PubMed]
  • Kohan S, Fröysa B, Cederlund E, Fairwell T, Lerner R, Johansson J, Khan S, Ritzen M, Jörnvall H, Cekan S, et al. Peptides of postulated inhibin activity. Lack of in vitro inhibin activity of a 94-residue peptide isolated from human seminal plasma, and of a synthetic replicate of its C-terminal 28-residue segment. FEBS Lett. 1986 Apr 21;199(2):242–248. [PubMed]
  • Gordon WL, Liu WK, Akiyama K, Tsuda R, Hara M, Schmid K, Ward DN. Beta-microseminoprotein (beta-MSP) is not an inhibin. Biol Reprod. 1987 May;36(4):829–835. [PubMed]
  • Brar A, Mbikay M, Sirois F, Fournier S, Seidah NG, Chrétien M. Localization of the human prostatic secretory protein PSP94 and its mRNA in the epithelial cells of the prostate. J Androl. 1988 Jul-Aug;9(4):253–260. [PubMed]
  • Ulvsbäck M, Lindström C, Weiber H, Abrahamsson PA, Lilja H, Lundwall A. Molecular cloning of a small prostate protein, known as beta-microsemenoprotein, PSP94 or beta-inhibin, and demonstration of transcripts in non-genital tissues. Biochem Biophys Res Commun. 1989 Nov 15;164(3):1310–1315. [PubMed]
  • Beksac MS, Khan SA, Eliasson R, Skakkebaek NE, Sheth AR, Diczfalusy E. Evidence for the prostatic origin of immunoreactive inhibin-like material in human seminal plasma. Int J Androl. 1984 Oct;7(5):389–397. [PubMed]
  • Doctor VM, Sheth AR, Simha MM, Arbatti NJ, Aaveri JP, Sheth NA. Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: comparison of its distribution in normal, benign and malignant prostates. Br J Cancer. 1986 Apr;53(4):547–554. [PMC free article] [PubMed]
  • Abrahamsson PA, Lilja H, Falkmer S, Wadström LB. Immunohistochemical distribution of the three predominant secretory proteins in the parenchyma of hyperplastic and neoplastic prostate glands. Prostate. 1988;12(1):39–46. [PubMed]
  • Sheth NA, Vaze AY, Sheth AR. A peptide in gastric secretion with inhibin-like properties. Clin Endocrinol (Oxf) 1982 Aug;17(2):157–163. [PubMed]
  • Vaze AY, Thakur AN, Sheth AR. Development of a radioimmunoassay for human seminal plasma inhibin. J Reprod Fertil Suppl. 1979;(26):135–146. [PubMed]
  • Dahlbäck B, Lundwall A, Stenflo J. Localization of thrombin cleavage sites in the amino-terminal region of bovine protein S. J Biol Chem. 1986 Apr 15;261(11):5111–5115. [PubMed]
  • Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. [PubMed]
  • Greider MH, Steinberg V, McGuigan JE. Electron microscopic identification of the gastrin cell of the human antral mucosa by means of immunocytochemistry. Gastroenterology. 1972 Oct;63(4):572–583. [PubMed]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...