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Proc Natl Acad Sci U S A. Jun 6, 2000; 97(12): 6914–6919.
PMCID: PMC18775
Population Biology

Changes in period mRNA levels in the brain and division of labor in honey bee colonies

Abstract

Previous research showed that age-related division of labor in honey bees is associated with changes in activity rhythms; young adult bees perform hive tasks with no daily rhythms, whereas older bees forage with strong daily rhythms. We report that this division of labor is also associated with differences in both circadian rhythms and mRNA levels of period, a gene well known for its role in circadian rhythms. The level of period mRNA in the brain oscillated in bees of all ages, but was significantly higher at all times in foragers. Elevated period mRNA levels cannot be attributed exclusively to aging, because bees induced to forage precociously because of a change in social environment had levels similar to normal age foragers. These results extend the regulation of a “clock gene” to a social context and suggest that there are connections at the molecular level between division of labor and chronobiology in social insects.

Age-related division of labor, the most common form of division of labor among workers in insect societies (1), is based on a pattern of behavioral development by individual workers. Workers typically spend the first part of their adult life working in the nest and the final part of their adult life foraging for food outside the nest (2). This division of labor is also flexible, as workers are able to respond to changes in colony needs by showing plasticity in the rate of behavioral development. In honey bees, where this plasticity has been studied most extensively, the pace of behavioral development is known to be influenced by environmental, social, and genetic factors (3). That division of labor is a highly regulated process is evident by the fact that behavioral development in honey bees involves changes in circulating hormone levels (4), brain chemistry (5, 6), and brain structure (7).

Changes in neural function that underlie long-term changes in memory are subserved by transcription-dependent mechanisms (8). This also might be the case for the long-term changes in neural function that underlie age-related division of labor in social insects. In animal species in general, there is as yet little evidence for changes in gene expression in the brain that are associated with behavioral plasticity in naturalistic contexts (912). There is only a single report of developmental regulation of gene expression in the bee brain: age-related increases in brain mRNA levels of royal jelly protein-3 (13), which is very similar to a protein produced by the hypopharyngeal glands and fed to the brood. The function of this protein in the brain is not known.

Studying “candidate genes” is one way to gain insight into the extent to which transcription-dependent mechanisms are involved in division of labor. To use this approach, it is necessary to choose a gene whose function in another organism may plausibly be related to division of labor. Our choice was guided by recent findings of intriguing changes in activity rhythms associated with division of labor. Forager honey bees have a highly developed internal circadian clock that is used for sun compass navigation, dance communication, and for timing visits to flowers for maximum nectar and pollen availability (14). However, tasks such as brood care are performed around the clock, arrhythmically (15, 16). Bees showed activity rhythms in the hive before they began to forage, and genotypes that resulted in younger ages at onset of foraging also showed an earlier onset of activity rhythms (16). Based on these findings, we hypothesized that division of labor in honey bee colonies also involves behavioral and molecular changes in circadian processes.

To determine whether the changes in diurnal activity rhythms observed under natural conditions (15, 16) reflect changes in endogenous circadian rhythms, we studied the locomotor activity of newly emerged adult bees under controlled laboratory conditions. We also tested the hypothesis that division of labor in honey bees is associated with changes in a molecular component of the circadian clock. We chose period (per) because in Drosophila melanogaster it shows pleiotropy for temporal phenomena. Mutations of per affect circadian locomotor rhythms (17), mating behavior (18), and development time from egg to adult (19), processes with dramatically different timescales. Honey bee behavioral development—from hive tasks to foraging—is not a rhythmic process, but it has a strong temporal component. Here, we report on changes in per mRNA levels in the brain associated with division of labor.

Materials and Methods

Bees.

Bee colonies were maintained in the field according to standard commercial techniques at the University of Illinois Bee Research Facility (Urbana, IL). The colonies in this region are derived from a mixture of European races of Apis mellifera. Bees used in all experiments were from colonies headed by queens that were each instrumentally inseminated with semen from a single (different) drone. Different colonies were used in each experiment; in some experiments, a colony was used as a “source” to make smaller experimental colonies. Sample sizes for bees and colonies are given for each experiment.

Measuring the Ontogeny of Circadian Rhythms for Locomotor Activity.

Care was taken to minimize exposure of bees to extrinsic factors that could entrain activity rhythms. We collected frames of pupae from colonies in the field and placed them in a light-proof container in an incubator (33°C, 95% relative humidity). Under brief exposure to dim red light (which bees cannot see; ref. 14), we transferred 0- to 24-h-old adult bees to individual clear Plexiglas cages with sugar syrup, pollen substitute, and water ad libitum and maintained in constant darkness (DD) at 26°C in an environmental chamber. Locomotor activity was monitored automatically with an infrared sensor (20) and recorded by the Datacol 3 acquisition system (Mini-Mitter, Sunriver, OR). We determined whether patterns of activity had circadian rhythms in the first experiment by eye with double-plotted data (20) and in experiments 2 and 3 first by eye and then with χ2 periodogram analysis (Tau program; Mini-Mitter) (21). The criterion for the onset of rhythmicity was set at P < 0.01; we used this criterion because periodic rhythms significant with a criterion of P < 0.05 were detected even in the absence of a single clearly defined peak of activity. This is probably because of the large sample sizes for each bee, which are a reflection of the high level of bee activity in the cages, and data being collected every 10 min. Using P < 0.01 as the criterion resulted in only those activity records with a single clearly defined peak of activity showing statistical significance. Experiment 1: n = 55 bees from 13 different colonies (≈4 bees per colony); experiment 2: n = 33 bees from colony R1; and experiment 3: n = 23 bees from colony R20.

Laboratory Colonies.

We measured brain mRNA per levels from bees collected from “single-cohort” colonies maintained in an environmental chamber (31°C day and 28°C night, 65% relative humidity). Each single-cohort colony was composed initially of 450–650 1-day-old bees. Colonies were entrained to light/dark (LD) 12:12. Fluorescent lights were positioned to ensure even lighting, minimizing the possibility that bees could avoid entrainment by hiding in dark corners of the hive (22). A queen pheromone strip (Bee Boost; Phero Tech, BC, Canada) was used instead of a live queen because a live queen can influence the rhythms of worker bees (23).

Brain mRNA per levels were measured from three age groups of bees. First, we collected young bees (4–6 days old) from three single-cohort colonies (one colony each from source colonies 19, 45, and 101) maintained for 4 days under LD (12:12) and for 2 days under DD. During DD, bees were exposed to dim red light produced by fluorescent bulbs specially coated (Chemical Products, North Warren, PA) to only emit 660- to 670-nm wavelengths. This light was used to allow for collections to be made during dark phases. Bees were collected on day 4 of LD and the 2 days of DD. We collected the next sample of young bees (7–9 days old) from three different single-cohort colonies (again, one colony each from source colonies 19, 45, and 101) maintained for 7 days under LD and for 2 days under DD. Bees were collected on day 7 of LD and the 2 days of DD. Finally, we collected old bees (20–22 days old) from two single-cohort colonies (from source colonies 45 and 101) maintained for 20 days under LD and for 2 days under DD. Bees were collected on day 20 of LD and the 2 days of DD. Old bees derived from source colony 19 were taken directly from the source colony itself in the field; the bees were entrained (12:12 LD) in the environmental chamber for 5 days and then were collected during day 6 of LD and 2 days of DD. For each age group, we collected bees every 4 h during the three collection days (n = 20 time points, 20 bees per time point). Bees were entrained under LD for a minimum of 4 days (S. M. Reppert, personal communication) because mRNA analyses required the use of material pooled from multiple bees for each data point. This protocol prevented us from analyzing bees younger than 4 days of age. We collected two groups of young bees to maximize chances of detecting differences relative to old bees.

Field Colonies.

To determine whether results obtained from laboratory colonies accurately reflect results that occur under more natural conditions, we also measured brain per mRNA levels from bees collected from one free-flying colony. These bees were from one of the same source colonies used above (source colony 45). We marked 1-day-old bees (n = 500) with a spot of paint on the thorax and introduced them into an unrelated colony in a standard hive maintained indoors at 27°C with an opening to the outside. The colony was exposed to natural sunlight at the hive entrance (LD cycle ≈12:12) and allowed free flight; it was kept indoors to enable us to efficiently remove bees during nocturnal collections. We collected marked bees from honeycombs containing brood in the hive at 7 days of age (under red light) and as returning foragers 17–24 days of age at the hive entrance every 3 h during 1 day. Some marked foragers were also marked with an additional paint dot as they entered the hive and then were sampled from combs during the nocturnal collections.

To determine whether differences in brain per mRNA levels detected in laboratory and field colonies are associated solely with differences in age, we also measured brain mRNA levels from bees taken from two single-cohort colonies in the field. These single-cohort colonies each consisted of ca. 2,000 1-day-old bees, a queen, and three frames of comb, two partially filled with honey and pollen, and one empty for the queen to lay eggs (24). Each single-cohort colony was made from a different source colony than in previously described experiments. About 5–10% of the members of a single-cohort colony develop into precocious foragers about 2 wk earlier than usual because older bees are not present to inhibit their behavioral development (24, 25). We collected precocious and normal-age foragers every 6 h for 1 day when they were 10 and 22 days old, respectively. (The laboratory colonies described above also were single-cohort colonies, but we did not expect that sampling them at early ages would be compromised by the presence of precociously developing bees; they had very small populations and few bees in a single-cohort colony are precocious.)

Individually Reared Bees.

To determine whether differences in brain per mRNA levels detected in laboratory and field colonies completely depend on the social environment, we reared bees individually in the laboratory, as described above. One-day-old bees (from a different source colony than in previously described experiments) were kept in the laboratory at 26°C for 6 days [3 days of LD 15:9 (similar to natural conditions at that time of year) and 3 days of DD]. Foragers were collected in the field from the same colony and kept in the laboratory for 3 days (DD). Bees were collected every 6 h the following day.

Molecular Cloning of a Honey Bee Ortholog of Period.

Total RNA was isolated from bee brains (RNeasy total RNA isolation kit; Qiagen, Valencia, CA) followed by treatment with DNase (1 unit RQ1 Dnase; Promega). mRNA was isolated with the Poly(A) Quik kit (Stratagene). First strand synthesis was done according to GIBCO/BRL protocols for Superscript II using 200 ng of poly(A) RNA, 150 ng of random hexamers, and 200 units of Superscript reverse transcriptase. PCR was with degenerate primers based on amino acid sequences CVISMH and YNQLNYN (26) and conditions reported therein (except final primer concentration 400 nM and one set of 35 cycles). A 1,083-bp PCR fragment was sequenced, and a blast search of GenBank revealed that it has significant amino acid similarity to per from other insects: 58% for Periplaneta americana, 55% for Drosophila melanogaster, and 48% for Hyalophora cecropia and Manduca sexta. The full-length sequence for cDNA (AmPer) was obtained by probing a UniZAP vector honey bee brain cDNA library (made for us by Stratagene) with the PCR fragment. AmPer shows several regions of similarity to per in all other species, including the presence of the PAS domain, which mediates interactions between PER and other clock proteins (27). The GenBank accession number for the full cDNA and amino acid sequence is AmPer AF159569.

Quantification of mRNA. Slot blot analysis.

Bees were collected directly into liquid N2, and brains were dissected on dry ice. Total brain RNA was isolated (as above), and 7.5 μg and 1.5 μg, respectively, were used for blots with per and elongation factor 1 alpha (EF-1α) as a control (28). We used a 1,083-bp per riboprobe from the PCR fragment, which spanned most of the c2 region (29), and a 600-bp honey bee EF-1α riboprobe (from a clone given to us by P. R. Ebert, University of Queensland). Both were digoxygenin (DIG)-11-UTP labeled and were used with DIG Easy Hyb buffer (Boehringer Mannheim, manufacturer's protocols); per blots were probed at 62°C with 35 ng/ml probe.

Northern blot analysis.

To verify the results of slot blot analyses, we also measured levels of brain per mRNA with Northern blot analysis. Total RNA (5 μg), extracted from 4–10 brains per time point, was separated on 1% agarose/0.6 M formaldehyde gels. Filters were first probed with per and then stripped and reprobed with EF-1α. Procedures and probes were as described above.

Statistical analyses.

More than 2,000 brains were dissected and pooled (4–10 per time point) for mRNA quantification. For laboratory colonies, we used split-plot ANOVA with colony and age as whole plots, LD/DD as split levels and time as reported within; for the field colony, two-way ANOVA (time and age); for field single-cohort colonies and individually reared bees, paired t tests; (SAS Institute, Cary, NC). For behavioral analyses, G tests were used to determine whether the proportion of bees with circadian locomotor rhythms increased with age.

Results

All individual bees reared in the laboratory were very active, but none showed any discernible circadian rhythm of locomotor activity for the first few days (Fig. (Fig.1).1). The mean (±SE) age at onset of circadian rhythm was 7.6 ± 0.6 in the first experiment (n = 55 bees from 13 different colonies, ≈4 bees per colony), 8.3 ± 0.4 in experiment 2 (n = 33 bees from colony R1), and 7.0 ± 0.6 days in experiment 3 (n = 23 bees from colony R20). The proportion of rhythmic bees increased significantly with age in experiments 1, 2, and 3 (P < 0.01, G tests) (Fig. (Fig.2).2).

Figure 1
Ontogeny of circadian rhythm in honey bee locomotor behavior. Representative actograms (Left) and periodogram analyses (Right) for two unrelated bees. Brackets indicate the arrhythmic and rhythmic portions of each actogram, as determined by eye, with ...
Figure 2
Age-related changes in percentage of bees showing circadian rhythms for locomotor behavior. For each bee, periodogram analysis was performed on data from successive 4-day intervals to determine when there was significant circadian rhythmicity (P < ...

Per mRNA cycled in bee brains in an endogenous circadian manner. Per mRNA levels oscillated in both young and old bees (Fig. (Fig.3)3) in laboratory colonies under conditions of DD (Table (Table1,1, significant “time” effect). Exposure to light did not cause significant variation in absolute levels (Table (Table1,1, LD/DD), again indicating endogenous regulation. These results are consistent with analyses of per in other species (27).

Figure 3
Differences in per mRNA levels in the brains of young and old bees. Representative RNA slot blots (A and B) from one of the three laboratory colonies studied and densitometric analyses (C) from all three colonies (National Institutes of Health image version ...
Table 1
Results of ANOVA for mRNA levels in the brains of young vs. old bees

There was a significant effect of age on per mRNA levels in the brain of bees from in laboratory colonies (Table (Table1).1). Young bees (4–6 days old and 7–9 days old) had per mRNA levels that were on average 45.8% and 26.3%, respectively, as high as old bees (Fig. (Fig.4).4). Differences in per mRNA levels between young and old bees were seen in all laboratory colonies despite the presence of significant intercolony variation in overall levels (Table (Table1)1) and phase and amplitude (Fig. (Fig.3).3).

Figure 4
Differences in per mRNA levels in the brains of young and old bees, 7-day-old hive bees and 17- to 24-day-old foragers and precocious and normal age foragers. Means (±SE) of colony means given for laboratory (n = 3) and single-cohort (n = 2) colonies; ...

There also were significant differences in per brain mRNA levels in bees collected from a field colony maintained under more natural conditions (Table (Table1).1). mRNA levels in 7-day-old bees were significantly lower than in foragers (Table (Table1),1), only 45.5% as high (Fig. (Fig.4).4). Northern blot analysis confirmed that foragers had significantly higher per levels than young bees (Fig. (Fig.5),5), even after social isolation for several days.

Figure 5
Differences in mRNA levels in the brains of young laboratory-reared bees and foragers. Northern blot (A) and densitometric analyses (B) (normalization as in Fig. Fig.3).3). Result of statistical analyses (paired t test) indicated on graph (n = ...

The increase in per mRNA levels in the brain was not strictly associated with aging. Precocious foragers from two single-cohort colonies maintained in the field had levels of per mRNA that were not significantly different from normal age foragers (P > 0.81; Fig. Fig.44).

Discussion

Honey bees behave arrythmically during the first part of their adult life when they work at tasks that require around-the-clock activity. They then show strong rhythmic behavior before the onset of foraging and use their circadian clock for navigation and foraging-related activities (16). We showed that this change reflects an ontogeny of endogenous circadian rhythmicity. Postembryonic development of circadian behavioral rhythms has not been observed in other insects but does occur in reptiles, birds, and mammals (30). The absence of circadian behavioral rhythms in very young mammals is thought to be a result of a lack of entrainment rather than a lack of circadian function in the superchiasmatic nuclei, the location of the mammalian circadian clock (31). Eusocial naked mole rats that live their entire life as nest workers apparently have no circadian rhythm of activity, whereas dispersing males that leave the colony do (32). Behavioral results from bees and naked mole rats suggest that social evolution can influence the functioning of circadian rhythm systems.

We also demonstrated that changes in the expression of a clock gene are associated with age-related division of labor in a social insect. Although there is both development of rhythmic locomotor activity and change in the levels of brain per mRNA, we cannot conclude that these two processes are connected because they were not measured in the same bees. Based on the data presented in Fig. Fig.2,2, it is likely that many, but not all, of the young bees used for mRNA analyses were arrhythmic; it was not possible to measure per levels in younger bees because of the constraints of the experimental design.

With the recent cloning of other genes that interact with per, the mechanism for rhythmic cycling of per transcription is beginning to be understood, and many of the molecules involved are conserved in mammals and insects (27). Future models of per regulation also will need to explain the phenomenon reported here: increases in per mRNA levels in the bee brain that occur during behavioral maturation even as cycling persists. The increase in per mRNA in the bee brain was associated with foraging, but it cannot be caused solely, if at all, by increased chronological age, foraging, flight, or exposure to the sun. Bees in colonies confined to the laboratory also had high, forager-like, levels of per, and foragers isolated from their social environment and maintained in darkness for several days still maintained high per levels. We speculate that levels of brain per mRNA increase in anticipation of the challenges of foraging. Perhaps higher levels of per are required to engage clock-controlled systems in the bee that are required for efficient social foraging, such as time-compensated sun compass navigation, dance communication, and the ability to time visits to flowers.

Our speculation is consistent with results showing that transgenic manipulations in Drosophila that result in changes in per mRNA dynamics also influence circadian rhythms (33, 34). The general notion that per is involved in a more diverse set of biological phenomena than previously recognized is supported by recent findings (35) of unexpected roles for per and other clock genes in cocaine sensitization and the similarity of a Caenorhabditis elegans developmental timing protein to proteins encoded by clock genes (36). A key first step in exploring the functional significance of the per increase in bees is to determine whether a similar increase occurs for the PERIOD protein, and if so, in what cells in the brain. Preliminary results (37) suggest that there is an age-related increase in the number of PERIOD-immunoreactive cells in the bee brain.

At present, it is not feasible to determine definitively the function of the per increase in bees because techniques to manipulate the honey bee genome are not yet available. However, it is possible to determine whether socially mediated mechanisms that control the onset of foraging, such as juvenile hormone, biogenic amines, and primer pheromones (3, 5), are associated with the expression of per. The only factors known to influence the expression of any clock gene were light, temperature, and other clock genes (27); recently a behaviorally induced phase advance of the circadian clock was shown to also cause rapid down-regulation of per expression (38). Our results demonstrate that new challenges to the understanding of clock genes emerge by studying animals that live in complex societies.

Acknowledgments

We thank J. Magner for designing the infrared sensor circuits; S. M. Reppert for generously providing primers and advice on experimental design; PheroTech for queen pheromone strips; J. C. Hall and M. W. Young for helpful discussions; J. Koenig, I. Lederhendler, and M. Sesma for timely support; Y. Ben-Shahar, T. Giray, J. C. Kuehn, A. J. Ross, D. J. Schulz, and C. W. Thompson for assistance with the bees; P. R. Ebert for the EF-1α clone; S. Aref (University of Illinois Statistics Consulting Services) for advice; D. J. Schulz for help with statistical programs; and Y. Ben-Shahar, E. H. Brown, E. A. Capaldi, D. F. Clayton, M. M. Elekonich, S. E. Fahrbach, S. M. Farris, M. L. U. Gillette, C. P. Kyriacou, H. M. Robertson, M. Rosbash, D. J. Schulz, and C. W. Thompson for reviewing this manuscript. This work was supported by a National Institute of Mental Health predoctoral fellowship (to D.P.T.), a National Science Foundation Research Opportunity Award (to D.M.) (in conjunction with a National Science Foundation grant to S. E. Fahrbach and G.E.R.), Fulbright and U.S.-Israel BARD postdoctoral fellowships (to G.B.), and grants from the National Institutes of Health, National Science Foundation, and University of Illinois Research Board (to G.E.R.).

Abbreviations

per
period
EF-1α
elongation factor 1α
DD
constant darkness
LD
light/dark

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