• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of genesdevCSHL PressJournal HomeSubscriptionseTOC AlertsBioSupplyNetGenes & Development
Genes Dev. Sep 15, 2002; 16(18): 2339–2344.
PMCID: PMC187443

Nodal activity in the node governs left-right asymmetry

Abstract

Nodal is expressed at the lateral edges of the mouse node, but its function in this “organizer” tissue remains unknown due to the early lethality of Nodal mutant embryos. Here we used a genetic strategy to selectively remove Nodal activity from the node. Embryos lacking Nodal in the node fail to initiate molecular asymmetry in the left lateral plate mesoderm and exhibit multiple left-right patterning defects. Nodal may also act as a short-range signal to establish a functional midline barrier. Our findings confirm that the mouse node is instrumental in initiating left-right axis specification and identify Nodal as the key morphogen regulating this process.

Keywords: Nodal, node, left-right asymmetry, isomerism, lateral plate mesoderm, mouse

Genetic pathways controlling invariant situs of the major internal organ systems have been described in several vertebrate model systems (for review, see Capdevila et al. 2000; Hamada et al. 2002). In all cases, expression of the TGF-β signaling molecule, Nodal, is restricted to the left side of the embryonic axis, where its activity is critical for specifying the embryonic left-right (LR) axis. The upstream cellular and molecular mechanisms that direct asymmetric gene expression have been extensively described in chick. However, relatively little is known about how embryonic symmetry is broken, and how the LR signaling cascade that ultimately controls the positioning and morphogenesis of the internal organs is activated in mammalian embryos.

The mouse node is a bilaminar structure found at the rostral end of the primitive streak and has equivalent secondary axes inducing properties as Spemann's organizer in Xenopus (for review, see Beddington and Robertson 1999). It consists dorsally of epiblast and ventrally of the most caudal aspect of the notochordal plate. Several recent studies indicate that the mouse node plays an important role in the establishment of LR asymmetry (Nonaka et al. 1998; Okada et al. 1999). Ultrastructural studies have shown that monocilia present on cells of the exposed ventral surface of the node rotate in a counterclockwise direction and have been suggested to generate a net leftward flow of extraembryonic fluid known as the nodal flow (Sulik et al. 1994; Nonaka et al. 1998). Laterality defects documented in several mouse mutants that lack cilia, or whose cilia motility is compromised, have lead to the hypothesis that the nodal flow results in the localized accumulation of morphogen(s) on the left side of the node, thereby initiating the LR signaling cascade (Hamada et al. 2002). In keeping with this, artificially reversing the direction of nodal flow in cultured embryos is sufficient to reverse situs (Nonaka et al. 2002). Surgical ablation of the node (Davidson et al. 1999) and mutations affecting node formation (Dufort et al. 1998) both lead to embryonic laterality defects, implying that the node functions as a signaling center during LR axis formation. The node gives rise to midline structures such as the notochord and floor plate that act as a midline barrier necessary for maintaining correct laterality (Dufort et al. 1998; King et al. 1998; Meno et al. 1998). The midline barrier is proposed to prevent interference between the distinct signaling cascades on either side of the embryo. For example, in Lefty1 mutant embryos, loss of Lefty1 from the floor plate compromises the barrier, leading to bilateral Nodal expression in the lateral plate mesoderm and ultimately left isomerisms of the viscera (Meno et al. 1998).

Nodal is required in the pregastrulation embryo to establish the anterior-posterior axis (Brennan et al. 2001). Nodal expression at the extreme lateral edges of the ventral node (Collignon et al. 1996; Lowe et al. 1996) also make it an ideal candidate morphogen asymmetrically localized by the nodal flow to activate left-sided gene expression. However, this possibility has been impossible to test because Nodal mutant embryos arrest prior to primitive streak formation. Elegant studies in zebrafish have shown that a Nodal homolog, Squint, is capable of acting as a classical morphogen, influencing the fate of cells at a distance from the source of Squint production (Chen and Schier 2001). Here we investigated whether Nodal signals from the node similarly act on adjacent cell populations, by genetically removing Nodal activity from cells within the node. Strikingly, embryos lacking Nodal in the node exhibit an array of abnormalities consistent with defects in specification of the LR axis. The loss of Nodal within the node disrupts molecular asymmetry in the left lateral plate mesoderm (LPM). These findings strongly suggest that Nodal acts as a long-range morphogen. In addition, cell lineage studies demonstrate that Nodal acts locally to pattern the node-derived lineage, the notochord, which consequently induces the midline barrier.

Results and Discussion

At the 4–5-somite stage, Nodal expression becomes transiently asymmetric in the node with increased numbers of cells expressing Nodal on the left versus the right side (Collignon et al. 1996). A 2.7-kb region upstream of the Nodal locus directs expression in the initial symmetrical domain (Fig. (Fig.1D;1D; Norris and Robertson 1999). We further mapped this node enhancer to a 350-bp fragment that is sufficient to direct Nodal expression in the nodes of transgenic mice (Fig. (Fig.1E).1E). Removal of this 350-bp sequence from the 2.7-kb transgene largely abolishes expression in the node (Fig. (Fig.1F).1F). Weak punctate expression seen in more central ventral cells of the node potentially reflects the presence of a repressor element within the 350-bp region that normally downregulates Nodal expression in this subpopulation. Alignment of this 350-bp minimal enhancer with the corresponding region from the human Nodal locus identifies a 110-bp stretch that shares 87% identity (Fig. (Fig.1B),1B), including a predicted binding site for Foxa2 (Hnf3β), a member of the forkhead family of transcription factors expressed throughout the ventral cells of the node (Sasaki and Hogan 1993). The lack of Nodal expression in chimeric embryos generated from Foxa2 null ES cells (Dufort et al. 1998), and the pronounced genetic interaction between Foxa2 and Nodal (Collignon et al. 1996; Varlet et al. 1997) provide evidence that Foxa2 acts as an upstream activator of Nodal expression in the node.

Figure 1
Characterization of the Nodal node enhancer. Ventral (CJ) and lateral (K) views of approximately 8.0-d embryos at the various somite (s) stages indicated. (A) Map of Nodal locus illustrating location of node enhancer element (red circle) and ...

Previous fate mapping experiments using the lipophilic dye DiI suggest that the ventral layer of the node gives rise to multiple cell types including notochord, the floor plate of the neural tube, and the definitive endoderm (Beddington 1994; Sulik et al. 1994; Kinder et al. 2001). Here we have exploited an in vivo cell marking system to precisely map the fate of cells expressing Nodal in the node. Transgenic mice were generated that express Cre recombinase under the control of the 2.7-kb node enhancer (Fig. (Fig.1G).1G). These Nodal-Cre transgenic mice (Tg 2.7-Cre) were crossed to the Rosa-26 conditional reporter strain carrying a silent LacZ reporter allele that is specifically activated in cells expressing Cre-recombinase (Soriano 1999). As assessed by whole-mount in situ analysis of a panel of embryos (n = 15), Tg 2.7-Cre directs Cre expression in the node at the 0-somite stage. However, β-gal activity is consistently not detected until the 2–3-somite stage, indicating an approximately 3-h time delay between Cre transcription and reporter activity (Fig. (Fig.1H,J).1H,J). Analysis of reporter embryos at various stages of development confirms that the ventral node cells are relatively quiescent, as few descendants are labeled despite rapid growth at these embryonic stages (Bellomo et al. 1996). Although the lag in reporter activity compared to Cre expression makes it difficult to precisely delineate the anterior extent of notochord colonization by derivatives of the node, we only see labeled cells caudal to the hindlimb buds. Contrary to previous DiI labeling experiments, our data show that descendants of the outermost cells of the ventral node exclusively colonize the posterior notochord and do not contribute to either the floor plate or definitive endoderm (Fig. (Fig.11L,M).

To test whether Nodal activity is required during specification of the notochord lineage, we designed a strategy to specifically eliminate expression from the node. The 2.7-kb enhancer region was thus deleted by homologous recombination, and correctly targeted ES cells were used to generate germline chimeras (Fig. (Fig.2).2). Mice heterozygous for the 2.7-kb deletion (NodalΔ/+) are viable and fertile. Moreover, intercross matings yielded NodalΔ/Δ homozygous offspring at Mendelian frequencies (Table (Table1A).1A). Transcripts were examined by whole-mount in situ to confirm that removal of the 2.7-kb node enhancer element eliminates Nodal expression from this tissue. Nodal expression was unaffected during early gastrulation stages (data not shown). By early somite stages, however, node expression was greatly diminished but not completely eliminated, implicating additional as yet unmapped regulatory element(s) (Fig. (Fig.4B,4B, see below). Alternatively, residual expression may reflect the activity of the intronic Foxh1-dependent feedback enhancer (Norris and Robertson 1999), which we showed to be responsible for the asymmetric expression of Nodal in the node (Norris et al. 2002). This feedback enhancer directs barely detectable levels of reporter expression in the node (J. Brennan, D. Norris, and E. Robertson, unpubl.). Although expression of Nodal in the node is markedly attenuated in NodalΔ/Δ embryos, robust levels of Nodal expression in the left LPM (Fig. (Fig.4G,4G, see below) allow mutant embryos to develop with normal situs.

Figure 2
Targeted deletion of the Nodal node enhancer. (A) Map of Nodal genomic locus. (B) Targeting vector designed to replace 2.7 kb of Nodal sequence with a single loxP site (black triangle). (C) Targeted allele. (D) Recombined allele after Cre-mediated excision ...
Table 1
Breeding data for NodalΔ allele
Figure 4
Molecular asymmetry is not established in the lateral plate mesoderm of NodalΔ/− deletion mutants. Whole-mount in situ hybridization (AC,FL) and LacZ reporter staining (D,E) of 7.5-d (AC) and 8.0-d (D ...

We previously documented a highly dose-dependent requirement for Nodal signaling during anterior-posterior axis formation (Norris et al. 2002). In these experiments, reduced levels of Nodal signaling in the left LPM failed to activate Lefty2 yet still allowed activation of Pitx2. To test for similar dosage effects in the node, we crossed NodalΔ/+ heterozygotes to mice heterozygous for a null allele (Nodal+/−). We found that NodalΔ/− mutants were born at the correct Mendelian ratio but none survived beyond the first few hours of birth (Table (Table1B).1B). Interestingly, these mutants display an array of abnormalities consistent with defects in specification of the LR axis (Table (Table2).2). For example, in wild-type animals the stomach is positioned on the left (Fig. (Fig.3A),3A), whereas in NodalΔ/− mutants, positioning was randomized with 8 of 11 animals having the stomach positioned on the right (Fig. (Fig.3B).3B). Regardless of stomach orientation, spleens in the NodalΔ/− mutants were invariably reduced in size compared to control littermates (Fig. (Fig.3C,D).3C,D). LR patterning defects were also evident in the thoracic cavity. Normally, asymmetric branching results in the formation of four lung lobes on the right and a single lobe on the left (Fig. (Fig.3E).3E). However, the NodalΔ/− mutants consistently present right isomerisms having four lobes on the right and three or four lobes on the left (Fig. (Fig.3F).3F). Interestingly, in human laterality syndromes, this right pulmonary isomerism is typically associated with hyposplenia (Kosaki and Casey 1998).

Table 2
LR asymmetry defects in the viscera of NodalΔ/− mutants at birth
Figure 3
Left-right patterning defects of the viscera of NodalΔ/− deletion mutants. Organs were dissected from control and deletion pups on the day of birth. Left (L) and right (R) are as indicated in A and B. Normally the stomach is situated on ...

We used expression of α-cardiac actin to examine orientation of the heart tube. At 9.0 d, the direction of heart looping was randomized in NodalΔ/− mutants (Fig. (Fig.3G,H).3G,H). At birth, the apex of the heart normally points to the left side of the thoracic cavity (Fig. (Fig.3I).3I). However, in the NodalΔ/− mutants the apex of the heart points to the left, the middle (Fig. (Fig.3J),3J), or right. Regardless of overall orientation, hearts in NodalΔ/− animals show multiple abnormalities, including transposition of the major arteries (data not shown) and atrial-septal defects (Fig. (Fig.3J).3J). In all mutants examined (n = 10), the major common defect is the development of a common atrial chamber rather than separate left and right atria. This profound cardiac abnormality most likely accounts for the early postnatal lethality of the NodalΔ/ animals.

To understand the molecular basis of the laterality defects, we examined the expression of a number of LR marker genes by whole-mount in situ hybridization. At the early head fold stage, Nodal is normally expressed at the lateral edges of the notochordal plate (Fig. (Fig.4A).4A). Although the nodes of NodalΔ/− embryos are morphologically indistinguishable from control littermates (Fig. (Fig.4C‘;4C‘; data not shown), Nodal expression was barely, if at all, detectable (Fig. (Fig.4C).4C). Next, we used the NodallacZ reporter allele (Collignon et al. 1996) to examine the impact of eliminating Nodal expression in the node on the asymmetric expression of Nodal in the left LPM. At the 3–4-somite stage, NodallacZ is expressed in the node and the left LPM (Fig. (Fig.4D).4D). In contrast, expression of the NodallacZ reporter is confined to the node and fails to be induced in the LPM of NodalΔ/lacZ mutants (Fig. (Fig.4E,4E, Table Table3).3). The absence of asymmetric Nodal expression in the LPM was confirmed by analyzing Nodal mRNA levels (Fig. (Fig.4F,H).4F,H).

Table 3
Gene expression at the 3–5 somite stage of development

In those NodalΔ/− embryos in which Nodal mRNA is detected, asymmetric expression was retained in the node (Fig. (Fig.4H‘).4H‘). Together with the observation that deletion of the Foxh1-dependent enhancer leads to a loss of asymmetric Nodal expression (Norris et al. 2002), these findings confirm that activation of the feedback enhancer is responsible for asymmetric Nodal expression in the node. As suggested by the nodal flow hypothesis, asymmetric movement of fluid across the node potentially leads to local accumulation of Nodal, which in turn upregulates expression via the feedback enhancer. Cerberus is a secreted protein which has been shown to antagonize Nodal signaling in Xenopus assays (Piccolo et al. 1999). Nodal signaling on the right edge of the node is likely antagonized by the activity of the Cerberus-related factor Dante, which is expressed more strongly on the right-hand side of the node (Pearce et al. 1999). In combination, right-sided antagonism and the nodal flow potentially amplify differences in Nodal activity across the relatively small area of the node.

Our lineage analysis shows that cells expressing Nodal in the node are destined to form the notochord, the tissue that induces Lefty1 expression in the prospective floor plate (King et al. 1998). Foxa2 and Shh expression patterns demonstrate the presence of notochord and floor plate in NodalΔ/− mutants (data not shown). However, Lefty1 is not expressed in the prospective floor plate of NodalΔ/− mutants (Table (Table3;3; data not shown), suggesting that notochord progenitors in the ventral node are not patterned correctly. Alternatively, Nodal in the ventral layer of the node may induce Lefty1 expression in prospective floor plate precursors in the adjacent dorsal layer. Considering that deletion of the Foxh1-dependent enhancer leads to a loss of Lefty1 expression in the midline (Norris et al. 2002), NodalΔ/− mutants may fail to induce Lefty1 as a secondary defect associated with the loss of Nodal expression from the left LPM.

The TGF-β family member Lefty2 is a downstream target of Nodal signaling in the left LPM. As for Nodal, Lefty2 expression requires activation of the Foxh1-dependent enhancer (Saijoh et al. 2000). As predicted by the loss of Nodal signaling in the left LPM of NodalΔ/− mutants, Lefty2 expression is not activated in the LPM of mutants (Fig. (Fig.4J).4J). Nodal also induces expression of the homeobox gene Pitx2 in the left LPM (Shiratori et al. 2001). Pitx2 null mutant embryos resemble the NodalΔ/− mutants described here, and similarly exhibit pulmonary right-sided isomerisms, atrial septal defects, and hyposplenia (Gage et al. 1999; Kitamura et al. 1999; Lin et al. 1999; Lu et al. 1999). Pitx2 is not expressed in the left LPM of NodalΔ/− mutants, accounting for the laterality defects seen in some of the visceral organs (Fig. (Fig.4K,L).4K,L). In contrast to the Pitx2 mutants, cardiac looping is randomized in NodalΔ/− mutants. Thus downstream targets of Nodal signaling, other than Pitx2, likely control some aspects of organ laterality such as cardiac looping.

Transduction of Nodal signaling often requires the presence of a Nodal coreceptor of the EGF-CFC family on the recipient cell (Yeo and Whitman 2001). Animals carrying a null mutation in the EGF-CFC gene, Cryptic, exhibit laterality defects resembling those described here for NodalΔ/− embryos (Gaio et al. 1999; Yan et al. 1999). Nodal expression in Cryptic mutants is unaffected in the node but fails to be induced in the left LPM. We suggest that Cryptic selectively amplifies Nodal activity in the node. Another possibility is that Cryptic allows cells to sense a long-range Nodal signal in the LPM. Loss of GDF-1, a TGF-β ligand expressed in both the node and the LPM, also causes laterality defects (Rankin et al. 2000). GDF-1 expression is unaffected in the NodalΔ/− mutants (data not shown). An intriguing possibility is that Nodal and GDF-1, coexpressed in the node, may form heterodimers with distinct properties. Such an interaction has not yet been shown for GDF-1, but Nodal is capable of forming heterodimers with another TGF-β ligand, Bmp7 (Yeo and Whitman 2001). Alternatively, cross-talk between Nodal and GDF-1 signaling pathways in the node and/or LPM may enhance activation of downstream targets.

The ability of Nodal to act as a diffusible morphogen relaying a signal from the node to the LPM is unexpected, because previous studies suggest that the mature processed protein is unstable (Constam and Robertson 1999). However, Squint, a Nodal homolog in zebrafish, can signal over 6–8 cell diameters (Chen and Schier 2001). Moreover, studies of Lefty2 mutant mice suggest that long-range Nodal signaling activity is normally restricted by the feedback inhibitor Lefty2 (Meno et al. 2001). The mechanism for long-range activity is thought to be diffusion involving endocytosis (Sakuma et al. 2002). Alternatively, long-range Nodal signaling could involve a relay mechanism involving other unidentified molecules.

Materials and methods

Transgenic and targeted mouse strains

Transgenes were generated by introducing Nodal genomic sequences into the hsp68lacZpA reporter construct (Sasaki and Hogan 1996). Tg 2.7 contains a 2.7-kb BamH1-Spe1 fragment located approximately 7 kb upstream of Nodal coding exon 1. Tg 0.35 contains a 350-bp BglII fragment from Tg 2.7. Transgenic mice were produced as previously described (Norris and Robertson 1999).

The targeting construct for the node enhancer deletion (NodalΔ) contains a 4.1-kb (EcoRI-SpeI) 5′ homology arm and a 2.8-kb (BamHI-BamHI) 3′homology arm. The homology arms were cloned into a plasmid containing a loxP-flanked hygro selection cassette. The hygro cassette was excised from targeted ES cell lines by transient transfection of the pMC13-cre plasmid. Mice containing the Δ2.7 mutation were genotyped using the following primers: A3 (TGAACTCATGACCATCCTCC), C2 (CTGAGTGCTGGGATTATACC), and D2 (TTGCGAGCAATAGTCT CAGC).

Histology and RNA in situ hybridization

Embryos were genotyped prior to whole-mount in situ analysis by yolk sac PCR analysis.

Probes and protocols used for whole-mount in situ were as described (Norris et al. 2002).

Acknowledgments

We thank Liz Bikoff, Ray Dunn, and Cindy Lu for critical comments on the manuscript. This work was funded by the NIH (to E.J.R.) and supported by a Wellcome Prize Travelling Research Fellowship from the Wellcome Trust (J.B).

The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC section 1734 solely to indicate this fact.

Footnotes

E-MAIL ude.dravrah.saf@treborje; FAX (617) 496-6770.

Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1016202.

References

  • Beddington RSP. Induction of a second neural axis by the mouse node. Development. 1994;120:613–620. [PubMed]
  • Beddington RSP, Robertson EJ. Axis development and early asymmetry in mammals. Cell. 1999;96:195–209. [PubMed]
  • Bellomo D, Lander A, Harragan I, Brown NA. Cell proliferation in mammalian gastrulation: The ventral node and notochord are relatively quiescent. Dev Dyn. 1996;205:471–485. [PubMed]
  • Brennan J, Lu CC, Norris DP, Rodriguez TA, Beddington RSP, Robertson EJ. Nodal signaling in the epiblast patterns the early mouse embryo. Nature. 2001;411:965–969. [PubMed]
  • Capdevila J, Vogan KJ, Tabin CJ, Izpisua Belmonte JC. Mechanisms of left-right determination in vertebrates. Cell. 2000;101:9–21. [PubMed]
  • Chen Y, Schier AF. The zebrafish Nodal signal Squint functions as a morphogen. Nature. 2001;411:607–610. [PubMed]
  • Collignon J, Varlet I, Robertson EJ. Relationship between asymmetric nodal expression and the direction of embryonic turning. Nature. 1996;381:155–158. [PubMed]
  • Constam DB, Robertson EJ. Regulation of bone morphogenetic protein activity by pro domains and proprotein convertases. J Cell Biol. 1999;144:139–149. [PMC free article] [PubMed]
  • Davidson BP, Kinder SJ, Steiner K, Schoenwolf GC, Tam PPL. Impact of node ablation on the morphogenesis of the body axis and the lateral asymmetry of the mouse embryo during early organogenesis. Dev Biol. 1999;211:11–26. [PubMed]
  • Dufort D, Schwartz L, Harpal K, Rossant J. The transcription factor HNF3beta is required in visceral endoderm for normal primitive streak morphogenesis. Development. 1998;125:3015–3025. [PubMed]
  • Gage PJ, Suh H, Camper SA. Dosage requirement of Pitx2 for development of multiple organs. Development. 1999;126:4643–4651. [PubMed]
  • Gaio U, Schweickert A, Fischer A, Garratt AN, Muller T, Ozcelik C, Lankes W, Strehle M, Britsch S, Blum M, et al. A role of the cryptic gene in the correct establishment of the left-right axis. Curr Biol. 1999;9:1339–1342. [PubMed]
  • Hamada H, Meno C, Watanabe D, Saijoh Y. Establishment of vertebrate left-right asymmetry. Nat Rev Genet. 2002;3:103–113. [PubMed]
  • Kinder SJ, Tsang TE, Wakamiya M, Sasaki H, Behringer RR, Nagy A, Tam PPL. The organizer of the mouse gastrula is composed of a dynamic population of progenitor cells for the axial mesoderm. Development. 2001;128:3623–3634. [PubMed]
  • King T, Beddington RSP, Brown NA. The role of the brachyury gene in heart development and left-right specification in the mouse. Mech Dev. 1998;79:29–37. [PubMed]
  • Kitamura K, Miura H, Miyagawa-Tomita S, Yanazawa M, Katoh-Fukui Y, Suzuki R, Ohuchi H, Suehiro A, Motegi Y, Nakahara Y, et al. Mouse Pitx2 deficiency leads to anomalies of the ventral body wall, heart, extra- and periocular mesoderm and right pulmonary isomerism. Development. 1999;126:5749–5758. [PubMed]
  • Kosaki K, Casey B. Genetics of human left-right axis malformations. Semin Cell Dev Biol. 1998;9:89–99. [PubMed]
  • Lin CR, Kioussi C, O’Connell S, Briata P, Szeto D, Liu F, Izpisua-Belmonte JC, Rosenfeld MG. Pitx2 regulates lung asymmetry, cardiac positioning and pituitary and tooth morphogenesis. Nature. 1999;401:279–282. [PubMed]
  • Lowe LA, Supp DM, Sampath K, Yokoyama T, Wright CV, Potter SS, Overbeek P, Kuehn MR. Conserved left-right asymmetry of nodal expression and alterations in murine situs inversus. Nature. 1996;381:158–161. [PubMed]
  • Lu MF, Pressman C, Dyer R, Johnson RL, Martin JF. Function of Rieger syndrome gene in left-right asymmetry and craniofacial development. Nature. 1999;401:276–278. [PubMed]
  • Meno C, Shimono A, Saijoh Y, Yashiro K, Mochida K, Ohishi S, Noji S, Kondoh H, Hamada H. lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal. Cell. 1998;94:287–297. [PubMed]
  • Meno C, Takeuchi J, Sakuma R, Koshiba-Takeuchi K, Ohishi S, Saijoh Y, Miyazaki J, ten Dijke P, Ogura T, Hamada H. Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2. Dev Cell. 2001;1:127–138. [PubMed]
  • Nonaka S, Tanaka Y, Takeda S, Harada A, Kanai Y, Kido M, Hirokawa N. Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein. Cell. 1998;95:829–837. [PubMed]
  • Nonaka S, Shiratori H, Saijoh Y, Hamada H. Determination of left-right patterning of the mouse embryo by artificial nodal flow. Nature. 2002;418:96–99. [PubMed]
  • Norris DP, Robertson EJ. Asymmetric and node-specific nodal expression patterns are controlled by two distinct cis-acting regulatory elements. Genes & Dev. 1999;13:1575–1588. [PMC free article] [PubMed]
  • Norris DP, Brennan J, Bikoff EK, Robertson EJ. The FoxH1 dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. Development. 2002;129:3455–3468. [PubMed]
  • Okada Y, Nonaka S, Tanaka Y, Saijoh Y, Hamada H, Hirokawa N. Abnormal nodal flow precedes situs inversus in iv and inv mice. Mol Cell. 1999;4:459–468. [PubMed]
  • Overdier DG, Porcella A, Costa RH. The DNA-binding specificity of the hepatocyte nuclear factor 3/forkhead domain is influenced by amino-acid residues adjacent to the recognition helix. Mol Cell Biol. 1994;14:2755–2766. [PMC free article] [PubMed]
  • Pearce JJ, Penny G, Rossant J. A mouse cerberus/Dan-related gene family. Dev Biol. 1999;209:98–110. [PubMed]
  • Piccolo S, Agius E, Leyns L, Bhattacharyya S, Grunz H, Bouwmeester T, De Robertis EM. The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals. Nature. 1999;397:707–710. [PMC free article] [PubMed]
  • Rankin CT, Bunton T, Lawler AM, Lee SJ. Regulation of left-right patterning in mice by growth/differentiation factor-1. Nat Genet. 2000;24:262–265. [PubMed]
  • Saijoh Y, Adachi H, Sakuma R, Yeo CY, Yashiro K, Watanabe M, Hashiguchi H, Mochida K, Ohishi S, Kawabata M, et al. Left-right asymmetric expression of lefty2 and nodal is induced by a signaling pathway that includes the transcription factor FAST2. Mol Cell. 2000;5:35–47. [PubMed]
  • Sakuma R, Ohnishi Yi Y, Meno C, Fujii H, Juan H, Takeuchi J, Ogura T, Li E, Miyazono K, Hamada H. Inhibition of Nodal signaling by Lefty mediated through interaction with common receptors and efficient diffusion. Genes Cells. 2002;7:401–412. [PubMed]
  • Sasaki H, Hogan BLM. Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Development. 1993;118:47–59. [PubMed]
  • ————— Enhancer analysis of the mouse HNF-3 beta gene: Regulatory elements for node/notochord and floor plate are independent and consist of multiple sub-elements. Genes Cells. 1996;1:59–72. [PubMed]
  • Shiratori H, Sakuma R, Watanabe M, Hashiguchi H, Mochida K, Sakai Y, Nishino J, Saijoh Y, Whitman M, Hamada H. Two-step regulation of left-right asymmetric expression of Pitx2: Initiation by nodal signaling and maintenance by Nkx2. Mol Cell. 2001;7:137–149. [PubMed]
  • Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet. 1999;21:70–71. [PubMed]
  • Sulik K, Dehart DB, Iangaki T, Carson JL, Vrablic T, Gesteland K, Schoenwolf GC. Morphogenesis of the murine node and notochordal plate. Dev Dyn. 1994;201:260–278. [PubMed]
  • Varlet I, Collignon J, Norris DP, Robertson EJ. Nodal signaling and axis formation in the mouse. Cold Spring Harb Symp Quant Biol. 1997;62:105–113. [PubMed]
  • Yan YT, Gritsman K, Ding J, Burdine RD, Corrales JD, Price SM, Talbot WS, Schier AF, Shen MM. Conserved requirement for EGF-CFC genes in vertebrate left-right axis formation. Genes & Dev. 1999;13:2527–2537. [PMC free article] [PubMed]
  • Yeo C, Whitman M. Nodal signals to Smads through Cripto-dependent and Cripto-independent mechanisms. Mol Cell. 2001;7:949–957. [PubMed]

Articles from Genes & Development are provided here courtesy of Cold Spring Harbor Laboratory Press
PubReader format: click here to try

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...