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Appl Microbiol. 1975 Jan; 29(1): 1–6.
PMCID: PMC186899

Minimal Growth Requirements for Clostridium perfringens and Isolation of Auxotrophic Mutants


The minimal growth requirements for two strains of Clostridium perfringens were defined, and both synthetic and semisynthetic plating media were developed. Plate counts of the wild-type strains on both of these minimal media were equivalent to those on complex media. A number of auxotrophic mutants of each strain were isolated, and their phenotypes were defined.

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Selected References

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  • Dreyfus-Fourcade M, Sebald M, Zavadova M. Restriction et modification du phage r par "Clostridium perfringens" NCTC 8798 et ses mutants. Ann Inst Pasteur (Paris) 1972 Jun;122(6):1117–1127. [PubMed]
  • Duncan CL, Strong DH, Sebald M. Sporulation and enterotoxin production by mutants of Clostridium perfringens. J Bacteriol. 1972 Apr;110(1):378–391. [PMC free article] [PubMed]
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  • KAMEYAMA S, MURATA R, YAMADA T. Production of alpha toxin of Clostridium perfringens. I. Preparation of the reproducible peptone medium for the production of the toxin of high potency. Jpn J Med Sci Biol. 1956 Jun;9(3):81–91. [PubMed]
  • Riha WE, Jr, Solberg M. Chemically defined medium for the growth of Clostridium perfringens. Appl Microbiol. 1971 Oct;22(4):738–739. [PMC free article] [PubMed]
  • SMITH HW. The bacteriophages of Clostridium perfringens. J Gen Microbiol. 1959 Dec;21:622–630. [PubMed]
  • Ting MN, Fung DY. Chemically defined medium for growth and sporulation of Clostridium perfringens. Appl Microbiol. 1972 Nov;24(5):755–759. [PMC free article] [PubMed]

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