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Figure 2.

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Immunohistochemical detection of RET protein products in TCV. Seven of the TCV samples that were positive for RET/PTC rearrangements were analyzed by immunohistochemistry for the expression of the RET protein. As a control, 10 non-neoplastic thyroid samples and 5 TCV samples that did not bear RET/PTC rearrangements (RT-PCR) were analyzed. Representative examples are shown. A: Normal thyroid tissue. B: One TCV sample (same as in C) stained with H/E. C and D: Two independent TCV samples showing intense RET immunoreactivity in neoplastic cells. E and F: Same samples as in C and D: anti-RET was pre-incubated with a fivefold molar excess of the antigen as a control of the specificity of the reaction. G: protein lysates (100 μg) obtained from the indicated cell lines were immunoblotted with anti-RET, to assess antibody specificity or anti-γ-tubulin to assess loading levels.

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