• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Jun 1994; 62(6): 2562–2567.
PMCID: PMC186545

Identification of a novel antigen from Corynebacterium pseudotuberculosis that protects sheep against caseous lymphadenitis.


A 40-kDa protein antigen from Corynebacterium pseudotuberculosis has been identified by application of a strategy that employs locally derived antibody-secreting cells (ASC). ASC probes generated by culture of ASC obtained from the lymph node draining the site of infection showed a specificity restricted to a 40-kDa antigen. Analysis of immunoblots with sequential serum samples taken from sheep during the course of experimental primary infection with C. pseudotuberculosis also revealed the 40-kDa antigen as an early immunodominant antigen. Sheep vaccinated with two 100-micrograms doses of a 40-kDa antigen preparation in aluminium hydroxide adjuvant were protected against infection with C. pseudotuberculosis, with an 82% reduction in the proportion of infected sheep and a 98% reduction in lung lesions. Sera from vaccinated sheep exhibited a strong response only to the 40-kDa antigen on immunoblots. These results strongly suggest that the 40-kDa antigen plays a major role in immunity to caseous lymphadenitis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brogden KA, Chedid L, Cutlip RC, Lehmkuhl HD, Sacks J. Effect of muramyl dipeptide on immunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. Am J Vet Res. 1990 Feb;51(2):200–202. [PubMed]
  • Cameron CM, Minnaar JL, Purdom MR. Immunizing properties of Corynebacterium pseudotuberculosis cell walls. Onderstepoort J Vet Res. 1969 Dec;36(2):211–216. [PubMed]
  • CARNE HR, KATER JC, WICKHAM N. A toxic lipid from the surface of Corynebacterium ovis. Nature. 1956 Sep 29;178(4535):701–702. [PubMed]
  • Eggleton DG, Doidge CV, Middleton HD, Minty DW. Immunisation against ovine caseous lymphadenitis: efficacy of monocomponent Corynebacterium pseudotuberculosis toxoid vaccine and combined clostridial-corynebacterial vaccines. Aust Vet J. 1991 Oct;68(10):320–321. [PubMed]
  • Eggleton DG, Haynes JA, Middleton HD, Cox JC. Immunisation against ovine caseous lymphadenitis: correlation between Corynebacterium pseudotuberculosis toxoid content and protective efficacy in combined clostridial-corynebacterial vaccines. Aust Vet J. 1991 Oct;68(10):322–325. [PubMed]
  • Eggleton DG, Middleton HD, Doidge CV, Minty DW. Immunisation against ovine caseous lymphadenitis: comparison of Corynebacterium pseudotuberculosis vaccines with and without bacterial cells. Aust Vet J. 1991 Oct;68(10):317–319. [PubMed]
  • Ellis JA, Hawk DA, Mills KW, Pratt DL. Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate. Vet Immunol Immunopathol. 1991 Jul;28(3-4):303–316. [PubMed]
  • Ellis JA, Hawk DA, Mills KW, Pratt DL. Antigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis. Vet Immunol Immunopathol. 1991 Jul;28(3-4):289–301. [PubMed]
  • Hard GC. Examination by electron microscopy of the interaction between peritoneal phagocytes and Corynebacterium ovis. J Med Microbiol. 1972 Nov;5(4):483–491. [PubMed]
  • Hard GC. Comparative toxic effect of the surface lipid of Corynebacterium ovis on peritoneal macrophages. Infect Immun. 1975 Dec;12(6):1439–1449. [PMC free article] [PubMed]
  • Hodgson AL, Krywult J, Corner LA, Rothel JS, Radford AJ. Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle. Infect Immun. 1992 Jul;60(7):2900–2905. [PMC free article] [PubMed]
  • Jolly RD. The pathogenic action of the exotoxin of Corynebacterium ovis. J Comp Pathol. 1965 Oct;75(4):417–431. [PubMed]
  • Meeusen E, Brandon M. The use of antibody-secreting cell probes to reveal tissue-restricted immune responses during infection. Eur J Immunol. 1994 Feb;24(2):469–474. [PubMed]
  • Muckle CA, Gyles CL. Relation of lipid content and exotoxin production to virulence of Corynebacterium pseudotuberculosis in mice. Am J Vet Res. 1983 Jun;44(6):1149–1153. [PubMed]
  • Pearce EJ, James SL, Dalton J, Barrall A, Ramos C, Strand M, Sher A. Immunochemical characterization and purification of Sm-97, a Schistosoma mansoni antigen monospecifically recognized by antibodies from mice protectively immunized with a nonliving vaccine. J Immunol. 1986 Dec 1;137(11):3593–3600. [PubMed]
  • Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF. Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8703–8707. [PMC free article] [PubMed]
  • Smythe JA, Coppel RL, Brown GV, Ramasamy R, Kemp DJ, Anders RF. Identification of two integral membrane proteins of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5195–5199. [PMC free article] [PubMed]
  • Strasser M, Frey J, Bestetti G, Kobisch M, Nicolet J. Cloning and expression of a species-specific early immunogenic 36-kilodalton protein of Mycoplasma hyopneumoniae in Escherichia coli. Infect Immun. 1991 Apr;59(4):1217–1222. [PMC free article] [PubMed]
  • Tashjian JJ, Campbell SG. Interaction between caprine macrophages and corynebacterium pseudotuberculosis: an electron microscopic study. Am J Vet Res. 1983 Apr;44(4):690–693. [PubMed]
  • Walker J, Jackson H, Brandon MR, Meeusen E. Lymphocyte subpopulations in pyogranulomas of caseous lymphadenitis. Clin Exp Immunol. 1991 Oct;86(1):13–18. [PMC free article] [PubMed]
  • Wallis RS, Alde SL, Havlir DV, Amir-Tahmasseb MH, Daniel TM, Ellner JJ. Identification of antigens of Mycobacterium tuberculosis using human monoclonal antibodies. J Clin Invest. 1989 Jul;84(1):214–219. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...