• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of amjpatholAmerican Journal of Pathology For AuthorsAmerican Journal of Pathology SubscribeAmerican Journal of Pathology SearchAmerican Journal of Pathology Current IssueAmerican Journal of Pathology About the JournalAmerican Journal of Pathology
Am J Pathol. Sep 1996; 149(3): 933–943.
PMCID: PMC1865170

Tenascin-C and human tendon degeneration.

Abstract

We investigated the distribution of tenascin in supraspinatus tendons to determine whether an alteration in tenascin expression was associated with human tendon degeneration. Tenascin was present in all of the tendons studied, although with two distinct patterns of expression. First, tenascin was associated with organized, fibrous regions of the tendon matrix that were typical of the normal tendon structure. This distribution is consistent with a role for tenascin in collagen fibril organization, perhaps maintaining the interface between fibrils and adjacent structures. Second, although tenascin was generally absent from poorly organized matrix in degenerate tendons, it was strongly associated with some rounded cells in disorganized fibrocartilaginous regions that were more abundant in pathological specimens. Tenascin was also found around infiltrating blood vessels, with more intense staining associated with a mononuclear cell infiltrate. Western blotting of tendon extracts showed differences in tenascin isoform expression, with only the small (200-kd) tenascin isoform found in normal tendons. Degenerate tendons also expressed the 300-kd isoform, consistent with a role for the larger tenascin isoform in tendon disease, potentially stimulating tenocyte proliferation, cell rounding, and fibrocartilaginous change. Proteolytic fragments of tenascin were detected but only in ruptured tendons, an indication of matrix remodeling in degenerate tendons, with fragment sizes consistent with the activity of matrix metalloproteinase enzymes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cofield RH. Rotator cuff disease of the shoulder. J Bone Joint Surg Am. 1985 Jul;67(6):974–979. [PubMed]
  • Gelberman RH, Manske PR, Vande Berg JS, Lesker PA, Akeson WH. Flexor tendon repair in vitro: a comparative histologic study of the rabbit, chicken, dog, and monkey. J Orthop Res. 1984;2(1):39–48. [PubMed]
  • ELLIOTT DH. STRUCTURE AND FUNCTION OF MAMMALIAN TENDON. Biol Rev Camb Philos Soc. 1965 Aug;40:392–421. [PubMed]
  • Chard MD, Cawston TE, Riley GP, Gresham GA, Hazleman BL. Rotator cuff degeneration and lateral epicondylitis: a comparative histological study. Ann Rheum Dis. 1994 Jan;53(1):30–34. [PMC free article] [PubMed]
  • Riley GP, Harrall RL, Constant CR, Chard MD, Cawston TE, Hazleman BL. Glycosaminoglycans of human rotator cuff tendons: changes with age and in chronic rotator cuff tendinitis. Ann Rheum Dis. 1994 Jun;53(6):367–376. [PMC free article] [PubMed]
  • Riley GP, Harrall RL, Constant CR, Chard MD, Cawston TE, Hazleman BL. Tendon degeneration and chronic shoulder pain: changes in the collagen composition of the human rotator cuff tendons in rotator cuff tendinitis. Ann Rheum Dis. 1994 Jun;53(6):359–366. [PMC free article] [PubMed]
  • Cawston TE, Riley GP, Hazleman BL. Tendon lesions and soft tissue rheumatism--great outback or great opportunity? Ann Rheum Dis. 1996 Jan;55(1):1–3. [PMC free article] [PubMed]
  • Vogel KG, Koob TJ. Structural specialization in tendons under compression. Int Rev Cytol. 1989;115:267–293. [PubMed]
  • Chiquet M, Fambrough DM. Chick myotendinous antigen. II. A novel extracellular glycoprotein complex consisting of large disulfide-linked subunits. J Cell Biol. 1984 Jun;98(6):1937–1946. [PMC free article] [PubMed]
  • Gulcher JR, Nies DE, Marton LS, Stefansson K. An alternatively spliced region of the human hexabrachion contains a repeat of potential N-glycosylation sites. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1588–1592. [PMC free article] [PubMed]
  • Spring J, Beck K, Chiquet-Ehrismann R. Two contrary functions of tenascin: dissection of the active sites by recombinant tenascin fragments. Cell. 1989 Oct 20;59(2):325–334. [PubMed]
  • Mackie EJ, Halfter W, Liverani D. Induction of tenascin in healing wounds. J Cell Biol. 1988 Dec;107(6 Pt 2):2757–2767. [PMC free article] [PubMed]
  • Whitby DJ, Ferguson MW. The extracellular matrix of lip wounds in fetal, neonatal and adult mice. Development. 1991 Jun;112(2):651–668. [PubMed]
  • Leadbetter WB. Cell-matrix response in tendon injury. Clin Sports Med. 1992 Jul;11(3):533–578. [PubMed]
  • Laemmli UK, Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Mackie EJ, Tucker RP. Tenascin in bone morphogenesis: expression by osteoblasts and cell type-specific expression of splice variants. J Cell Sci. 1992 Nov;103(Pt 3):765–771. [PubMed]
  • Siri A, Knäuper V, Veirana N, Caocci F, Murphy G, Zardi L. Different susceptibility of small and large human tenascin-C isoforms to degradation by matrix metalloproteinases. J Biol Chem. 1995 Apr 14;270(15):8650–8654. [PubMed]
  • Rettig WJ, Erickson HP, Albino AP, Garin-Chesa P. Induction of human tenascin (neuronectin) by growth factors and cytokines: cell type-specific signals and signalling pathways. J Cell Sci. 1994 Feb;107(Pt 2):487–497. [PubMed]
  • Chiquet-Ehrismann R, Tannheimer M, Koch M, Brunner A, Spring J, Martin D, Baumgartner S, Chiquet M. Tenascin-C expression by fibroblasts is elevated in stressed collagen gels. J Cell Biol. 1994 Dec;127(6 Pt 2):2093–2101. [PMC free article] [PubMed]
  • Robbins JR, Vogel KG. Regional expression of mRNA for proteoglycans and collagen in tendon. Eur J Cell Biol. 1994 Aug;64(2):264–270. [PubMed]
  • Rufai A, Benjamin M, Ralphs JR. Development and ageing of phenotypically distinct fibrocartilages associated with the rat Achilles tendon. Anat Embryol (Berl) 1992 Dec;186(6):611–618. [PubMed]
  • Mackie EJ, Thesleff I, Chiquet-Ehrismann R. Tenascin is associated with chondrogenic and osteogenic differentiation in vivo and promotes chondrogenesis in vitro. J Cell Biol. 1987 Dec;105(6 Pt 1):2569–2579. [PMC free article] [PubMed]
  • Chuong CM, Chen HM. Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing. Am J Pathol. 1991 Feb;138(2):427–440. [PMC free article] [PubMed]
  • Salter DM. Tenascin is increased in cartilage and synovium from arthritic knees. Br J Rheumatol. 1993 Sep;32(9):780–786. [PubMed]
  • Mackie EJ, Ramsey S. Expression of tenascin in joint-associated tissues during development and postnatal growth. J Anat. 1996 Feb;188(Pt 1):157–165. [PMC free article] [PubMed]
  • Chuong CM, Widelitz RB, Jiang TX, Abbott UK, Lee YS, Chen HM. Roles of adhesion molecules NCAM and tenascin in limb skeletogenesis: analysis with antibody perturbation, exogenous gene expression, talpid mutants and activin stimulation. Prog Clin Biol Res. 1993;383B:465–474. [PubMed]
  • Fujii Y, Yoshida H, Sakou T. Immunohistochemical studies on tenascin in human yellow ligament. In Vivo. 1993 Mar-Apr;7(2):143–146. [PubMed]
  • Hoffman S, Crossin KL, Edelman GM. Molecular forms, binding functions, and developmental expression patterns of cytotactin and cytotactin-binding proteoglycan, an interactive pair of extracellular matrix molecules. J Cell Biol. 1988 Feb;106(2):519–532. [PMC free article] [PubMed]
  • Chevalier X, Groult N, Larget-Piet B, Zardi L, Hornebeck W. Tenascin distribution in articular cartilage from normal subjects and from patients with osteoarthritis and rheumatoid arthritis. Arthritis Rheum. 1994 Jul;37(7):1013–1022. [PubMed]
  • Sriramarao P, Bourdon MA. A novel tenascin type III repeat is part of a complex of tenascin mRNA alternative splices. Nucleic Acids Res. 1993 Jan 11;21(1):163–168. [PMC free article] [PubMed]
  • Weller A, Beck S, Ekblom P. Amino acid sequence of mouse tenascin and differential expression of two tenascin isoforms during embryogenesis. J Cell Biol. 1991 Jan;112(2):355–362. [PMC free article] [PubMed]
  • Oyama F, Hirohashi S, Shimosato Y, Titani K, Sekiguchi K. Qualitative and quantitative changes of human tenascin expression in transformed lung fibroblast and lung tumor tissues: comparison with fibronectin. Cancer Res. 1991 Sep 15;51(18):4876–4881. [PubMed]
  • Borsi L, Balza E, Castellani P, Carnemolla B, Ponassi M, Querzé G, Zardi L. Cell-cycle dependent alternative splicing of the tenascin primary transcript. Cell Adhes Commun. 1994 Jan;1(4):307–317. [PubMed]
  • Murphy-Ullrich JE, Lightner VA, Aukhil I, Yan YZ, Erickson HP, Hök M. Focal adhesion integrity is downregulated by the alternatively spliced domain of human tenascin. J Cell Biol. 1991 Nov;115(4):1127–1136. [PMC free article] [PubMed]
  • Chiquet-Ehrismann R, Matsuoka Y, Hofer U, Spring J, Bernasconi C, Chiquet M. Tenascin variants: differential binding to fibronectin and distinct distribution in cell cultures and tissues. Cell Regul. 1991 Nov;2(11):927–938. [PMC free article] [PubMed]
  • Tremble P, Chiquet-Ehrismann R, Werb Z. The extracellular matrix ligands fibronectin and tenascin collaborate in regulating collagenase gene expression in fibroblasts. Mol Biol Cell. 1994 Apr;5(4):439–453. [PMC free article] [PubMed]
  • Dalton S, Cawston TE, Riley GP, Bayley IJ, Hazleman BL. Human shoulder tendon biopsy samples in organ culture produce procollagenase and tissue inhibitor of metalloproteinases. Ann Rheum Dis. 1995 Jul;54(7):571–577. [PMC free article] [PubMed]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...