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Infect Immun. Feb 1994; 62(2): 596–605.
PMCID: PMC186146

Nonmotility and phagocytic resistance of Pseudomonas aeruginosa isolates from chronically colonized patients with cystic fibrosis.

Abstract

Although Pseudomonas aeruginosa chronically colonizes most older patients with cystic fibrosis (CF), bacterial features responsible for its persistence are understood poorly. We observed that many P. aeruginosa isolates from chronically colonized patients were nonmotile and resistant to phagocytosis by macrophages. P. aeruginosa isolates were collected from 20 CF patients for up to 10 years. Isolates from early colonization were highly motile and expressed both flagellin and pilin. However, many isolates from chronically colonized patients lacked flagellin expression and were nonmotile; a total of 1,030 P. aeruginosa CF isolates were examined, of which 39% were nonmotile. Moreover, sequential isolates recovered from several of the CF patients were consistently nonmotile for up to 10 years. Lack of motility was rare among environmental isolates (1.4%) and other clinical isolates (3.7%) of P. aeruginosa examined. Partial complementation of motility in nonmotile P. aeruginosa isolates was achieved by introduction of extra copies of the rpoN locus carried on plasmid pPT212, indicating that the alternate sigma factor, RpoN, may be involved in the coordinate regulation of virulence factors during CF infection. We hypothesize that the nonmotile phenotype may provide P. aeruginosa a survival advantage in chronic CF infection by enabling it to resist phagocytosis and conserve energy.

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  • Baltimore RS, Shedd DG. The role of complement in the opsonization of mucoid and non-mucoid strains of Pseudomonas aeruginosa. Pediatr Res. 1983 Dec;17(12):952–958. [PubMed]
  • Bradley DE, Pitt TL. Pilus-dependence of four Pseudomonas aeruginosa bacteriophages with non-contractile tails. J Gen Virol. 1974 Jul;24(1):1–15. [PubMed]
  • Burke V, Robinson JO, Richardson CJ, Bundell CS. Longitudinal studies of virulence factors of Pseudomonas aeruginosa in cystic fibrosis. Pathology. 1991 Apr;23(2):145–148. [PubMed]
  • Cabral DA, Loh BA, Speert DP. Mucoid Pseudomonas aeruginosa resists nonopsonic phagocytosis by human neutrophils and macrophages. Pediatr Res. 1987 Oct;22(4):429–431. [PubMed]
  • Campbell ME, Farmer SW, Speert DP. New selective medium for Pseudomonas aeruginosa with phenanthroline and 9-chloro-9-[4-(diethylamino)phenyl]-9,10-dihydro-10- phenylacridine hydrochloride (C-390). J Clin Microbiol. 1988 Sep;26(9):1910–1912. [PMC free article] [PubMed]
  • Chi E, Mehl T, Nunn D, Lory S. Interaction of Pseudomonas aeruginosa with A549 pneumocyte cells. Infect Immun. 1991 Mar;59(3):822–828. [PMC free article] [PubMed]
  • Drake D, Montie TC. Flagella, motility and invasive virulence of Pseudomonas aeruginosa. J Gen Microbiol. 1988 Jan;134(1):43–52. [PubMed]
  • Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648–1652. [PMC free article] [PubMed]
  • Hancock RE, Mutharia LM, Chan L, Darveau RP, Speert DP, Pier GB. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Infect Immun. 1983 Oct;42(1):170–177. [PMC free article] [PubMed]
  • Hancock RE, Poole K, Benz R. Outer membrane protein P of Pseudomonas aeruginosa: regulation by phosphate deficiency and formation of small anion-specific channels in lipid bilayer membranes. J Bacteriol. 1982 May;150(2):730–738. [PMC free article] [PubMed]
  • Hazlett LD, Moon MM, Singh A, Berk RS, Rudner XL. Analysis of adhesion, piliation, protease production and ocular infectivity of several P. aeruginosa strains. Curr Eye Res. 1991 Apr;10(4):351–362. [PubMed]
  • Høiby N, Rosendal K. Epidemiology of Pseudomonas aeruginosa infection in patients treated at a cystic fibrosis centre. Acta Pathol Microbiol Scand B. 1980 Jun;88(3):125–131. [PubMed]
  • Kelly NM, Kluftinger JL, Pasloske BL, Paranchych W, Hancock RE. Pseudomonas aeruginosa pili as ligands for nonopsonic phagocytosis by fibronectin-stimulated macrophages. Infect Immun. 1989 Dec;57(12):3841–3845. [PMC free article] [PubMed]
  • Krieg DP, Helmke RJ, German VF, Mangos JA. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect Immun. 1988 Dec;56(12):3173–3179. [PMC free article] [PubMed]
  • Kyhse-Andersen J. Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods. 1984 Dec;10(3-4):203–209. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Li C, Louise CJ, Shi W, Adler J. Adverse conditions which cause lack of flagella in Escherichia coli. J Bacteriol. 1993 Apr;175(8):2229–2235. [PMC free article] [PubMed]
  • Luzar MA, Montie TC. Avirulence and altered physiological properties of cystic fibrosis strains of Pseudomonas aeruginosa. Infect Immun. 1985 Nov;50(2):572–576. [PMC free article] [PubMed]
  • Luzar MA, Thomassen MJ, Montie TC. Flagella and motility alterations in Pseudomonas aeruginosa strains from patients with cystic fibrosis: relationship to patient clinical condition. Infect Immun. 1985 Nov;50(2):577–582. [PMC free article] [PubMed]
  • Mohr CD, Martin DW, Konyecsni WM, Govan JR, Lory S, Deretic V. Role of the far-upstream sites of the algD promoter and the algR and rpoN genes in environmental modulation of mucoidy in Pseudomonas aeruginosa. J Bacteriol. 1990 Nov;172(11):6576–6580. [PMC free article] [PubMed]
  • Mork T, Hancock RE. Mechanisms of nonopsonic phagocytosis of Pseudomonas aeruginosa. Infect Immun. 1993 Aug;61(8):3287–3293. [PMC free article] [PubMed]
  • Nelson JW, Tredgett MW, Sheehan JK, Thornton DJ, Notman D, Govan JR. Mucinophilic and chemotactic properties of Pseudomonas aeruginosa in relation to pulmonary colonization in cystic fibrosis. Infect Immun. 1990 Jun;58(6):1489–1495. [PMC free article] [PubMed]
  • Pasloske BL, Joffe AM, Sun Q, Volpel K, Paranchych W, Eftekhar F, Speert DP. Serial isolates of Pseudomonas aeruginosa from a cystic fibrosis patient have identical pilin sequences. Infect Immun. 1988 Mar;56(3):665–672. [PMC free article] [PubMed]
  • Pier GB, Meluleni G, Neuger E. A murine model of chronic mucosal colonization by Pseudomonas aeruginosa. Infect Immun. 1992 Nov;60(11):4768–4776. [PMC free article] [PubMed]
  • Saiman L, Ishimoto K, Lory S, Prince A. The effect of piliation and exoproduct expression on the adherence of Pseudomonas aeruginosa to respiratory epithelial monolayers. J Infect Dis. 1990 Mar;161(3):541–548. [PubMed]
  • Shi W, Li C, Louise CJ, Adler J. Mechanism of adverse conditions causing lack of flagella in Escherichia coli. J Bacteriol. 1993 Apr;175(8):2236–2240. [PMC free article] [PubMed]
  • Simpson DA, Ramphal R, Lory S. Genetic analysis of Pseudomonas aeruginosa adherence: distinct genetic loci control attachment to epithelial cells and mucins. Infect Immun. 1992 Sep;60(9):3771–3779. [PMC free article] [PubMed]
  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. [PubMed]
  • Smith RJ, Iden SS. Properties of calcium ionophore-induced generation of superoxide anion by human neutrophils. Inflammation. 1981 Sep;5(3):177–192. [PubMed]
  • Speert DP, Eftekhar F, Puterman ML. Nonopsonic phagocytosis of strains of Pseudomonas aeruginosa from cystic fibrosis patients. Infect Immun. 1984 Mar;43(3):1006–1011. [PMC free article] [PubMed]
  • Speert DP, Gordon S. Phagocytosis of unopsonized Pseudomonas aeruginosa by murine macrophages is a two-step process requiring glucose. J Clin Invest. 1992 Sep;90(3):1085–1092. [PMC free article] [PubMed]
  • Speert DP, Loh BA, Cabral DA, Salit IE. Nonopsonic phagocytosis of nonmucoid Pseudomonas aeruginosa by human neutrophils and monocyte-derived macrophages is correlated with bacterial piliation and hydrophobicity. Infect Immun. 1986 Jul;53(1):207–212. [PMC free article] [PubMed]
  • Speert DP, Wright SD, Silverstein SC, Mah B. Functional characterization of macrophage receptors for in vitro phagocytosis of unopsonized Pseudomonas aeruginosa. J Clin Invest. 1988 Sep;82(3):872–879. [PMC free article] [PubMed]
  • Starnbach MN, Lory S. The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin synthesis. Mol Microbiol. 1992 Feb;6(4):459–469. [PubMed]
  • Totten PA, Lara JC, Lory S. The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene. J Bacteriol. 1990 Jan;172(1):389–396. [PMC free article] [PubMed]
  • Valeyre D, Soler P, Basset G, Loiseau P, Pre J, Turbie P, Battesti JP, Georges R. Glucose, K+, and albumin concentrations in the alveolar milieu of normal humans and pulmonary sarcoidosis patients. Am Rev Respir Dis. 1991 May;143(5 Pt 1):1096–1101. [PubMed]
  • WAHBA AH, DARRELL JH. THE IDENTIFICATION OF ATYPICAL STRAINS OF PSEUDOMONAS AERUGINOSA. J Gen Microbiol. 1965 Mar;38:329–342. [PubMed]

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