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EMBO Rep. Apr 2007; 8(4): 408–413.
Published online Mar 9, 2007. doi:  10.1038/sj.embor.7400930
PMCID: PMC1852752
Scientific Report

Spliced-leader RNA silencing: a novel stress-induced mechanism in Trypanosoma brucei

Abstract

The signal-recognition particle (SRP) mediates the translocation of membrane and secretory proteins across the endoplasmic reticulum upon interaction with the SRP receptor. In trypanosomes, the main RNA molecule is the spliced-leader (SL) RNA, which donates the SL sequence to all messenger RNA through trans-splicing. Here, we show that RNA interference silencing of the SRP receptor (SRα) in Trypanosoma brucei caused the accumulation of SRP on ribosomes and triggered silencing of SL RNA (SLS). SLS was elicited due to the failure of the SL RNA-specific transcription factor tSNAP42 to bind to its promoter. SL RNA reduction, in turn, eliminated mRNA processing and resulted in a significant reduction of all mRNA tested. SLS was also induced under pH stress and might function as a master regulator in trypanosomes. SLS is reminiscent of, but distinct from, the unfolded protein response and can potentially act as a new target for parasite eradication.

Keywords: Trypanosoma brucei , signal recognition receptor;, SL RNA, trans-splicing

Introduction

In eukaryotes, translocation of secretory and membrane proteins across the endoplasmic reticulum is mediated primarily by the signal-recognition particle (SRP; Keenan et al, 2001). During protein translocation, the signal-peptide-binding protein of the SRP, SRP54, samples the signal peptide emerging from the ribosome nascent chain (RNC). The resulting SRP–RNC complex interacts with the SRP receptor SRα. Guanosine triphosphate (GTP) binding to SRα enhances the affinity between SRP and SR (Rapiejko & Gilmore, 1997). After signal sequence transfer to the translocon, both SRP54 and SRα act as reciprocal GTPase-activating proteins, resolving their association (Powers & Walter, 1995). The RNC binds to the membrane by interactions with the channel or Sec61 complex, and the protein is co-translationally translocated.

Trypanosomes are ancient protozoan parasites, which have a unique SRP that contains two RNA molecules—the 7SL RNA and a transfer RNA-like molecule—and lacks the Alu-domain-binding proteins (Liu et al, 2003; Lustig et al, 2005). The SRP pathway is essential for parasite survival (Liu et al, 2002; Lustig et al, 2005). Trypanosomes process their RNA by a unique mechanism, as all nuclear messenger RNA undergo trans-splicing, which involves the addition of a common spliced-leader (SL) sequence to the 5′ end of the mRNA. The source of the SL is a small RNA, the SL RNA. The SL RNA promoter is the only RNA polymerase II (pol II) promoter characterized in trypanosomes (Gilinger & Bellofatto, 2001; Das et al, 2005). It recruits the trypanosome snRNA-activating protein (tSNAP) complex, which contains three subunits: tSNAP26 and tSNAP50, which are related to factors that mediate small nuclear RNA (snRNA) transcription in metazoa, and tSNAP42, which is trypanosome specific (Das et al, 2005).

In this study, we show that the depletion of cells from the SRP receptor SRα elicits a new signalling pathway, leading to complete elimination of mRNA production through inhibition of SL RNA transcription. This novel pathway can also be induced under pH stress and might therefore be analogous to the unfolded protein response (UPR) that acts to protect cells from ambient stress (Schroder & Kaufman, 2005).

Results

SL RNA and mRNA production

To examine further the role of SRP in protein sorting, we knocked down the expression of the SRP receptor SRα by RNA interference (RNAi). The SRα gene was identified by homology searches of the Trypanosoma brucei genome. The gene (Tb11.01.1650) is composed of 582 amino acids, and shares 39% identity and 48% similarity to the human protein. Silencing was carried out by using expression of a stem–loop structure from an inducible promoter (Wang et al, 2000). Induction of SRα silencing was lethal and cells started dying 3 days after induction (Fig 1A). As a result of silencing, cells became ‘fat', as visualized by both confocal and scanning electron microscopy (SEM; Fig 1B). The level of SRα mRNA was reduced on silencing (Fig 1C). Interestingly, significant reduction of tubulin mRNA was also observed, which might explain the ‘fat' phenotype (Fig 1B).

Figure 1
Deleterious effects of signal-recognition particle receptor α silencing. (A) SRα is essential for cell growth. The growth of uninduced cells was compared with induced cells. The number of uninduced cells (−Tet) is shown by open ...

To investigate further the effect of SRα silencing on mRNA production, the level of several mRNAs was examined from cells 2–4 days after silencing (Fig 2A). In each case, the level of mRNA was reduced. The overall reduction in the level of mRNA is not due to total inhibition of RNA pol II-dependent transcription, as the level of EP procyclin, which is transcribed by RNA pol I, was similarly reduced (Fig 2A). The effect on mRNA was specific to SRα depletion, as no such effect was observed after SRP54 RNAi silencing. However, SRP54 depletion was efficient and its mRNA was completely eliminated (Fig 2A). Next, we examined whether the reduction of mRNA in SRα-depleted cells was due to changes in mRNA stability. Cells were treated with actinomycin D, before and after depletion, and the half-life of three mRNAs was determined. The results (supplementary Fig S1 online) show no effect on mRNA stability.

Figure 2
Signal-recognition particle receptor α silencing specifically inhibits spliced-leader RNA transcription and messenger RNA production. (A) The effect of SRα or SRP54 depletion on RNA level. RNA (20 μg) was prepared from uninduced ...

Inhibition of SL RNA synthesis, which is transcribed by pol II, should eliminate mRNA production by blocking trans-splicing. We examined next the level of SL RNA and compared it to other small RNAs transcribed by pol I and pol III, such as 5.8S ribosomal RNA, U6 snRNA and 7SL RNA. Significant RNA reduction was observed only for SL RNA (Fig 2A).

To examine trans-splicing during SRα depletion directly, the level of the Y structure intermediate was determined in steady-state mRNA. This resulted in two principal extension products: the Y structure and the mature capped SL RNA. The results (Fig 2B) indicate that the level of Y structure was reduced by 78%±5 compared with uninduced cells, and this corresponds to the decrease in the level of SL RNA. This suggests that during SRα depletion, the reduction in the Y structure is not the result of splicing defects, but is due to a reduction in the level of SL RNA.

Next we examined, using the permeable cell system, whether nascent SL RNA transcription is affected in the silenced cells (Tschudi & Ullu, 1990; Ullu & Tschudi, 1990). SL RNA is the major radiolabelled transcript in this system, but nascent tRNA, rRNA and mRNA can also be detected. The results (Fig 2C) indicated an 80%±4 reduction in the level of newly transcribed SL RNA. The decrease in the level of SL RNA was also reflected by a decrease in the level of the free intron and SL exon. This suggests that the mechanism/machinery of trans-splicing was not abrogated, and that the reduction reflects only a reduction in SL RNA production.

We wanted to explore further whether SRα depletion elicited a general shut-off of RNA transcription or whether transcription shut-off was specific to SL RNA. RNA extracted from permeable cells—before and after depletion—was analysed by slot-blot hybridization. We examined the level of rRNA, 7SL RNA and mRNA—tubulin and Hsp83—transcription, and compared it with that of the SL RNA (Fig 2D). The human gene Sirt6 was used as a control for nonspecific hybridization. Densitometric-based quantification of these data—based on long exposure—shows that mRNA levels were reduced by only 15–20% (as illustrated in Fig 2D), whereas SL RNA transcription—based on short exposure—was reduced by 82%±2 as a result of SRα depletion. Thus, there was a specific shut-off of SL RNA transcription as a consequence of the loss of SRα.

Changes in tSNAP complex during SRα depletion

To investigate how SL RNA transcription was specifically shut down, we examined some specific effects, such as amount and localization, on SL RNA transcription factors. Proteins present in nuclear extracts, made from cells before or after SRα knockdown, were studied by using western analysis. Figure 3A shows a significant reduction in the level of two of the tSNAPc subunits, tSNAP50 and tSNAP26 which most probably reflects mRNA elimination. Indeed, a significant reduction in protein synthesis examined by in vivo labelling was observed after SRα depletion (see supplementary Fig S2 online). By contrast, a significant increase in the third tSNAPc subunit, tSNAP42, was observed (Fig 3A). The localization of tSNAP42 showed a marked change in the subnuclear localization on silencing (Fig 3B). In uninduced cells, tSNAP42 localized to a distinct ‘dot', which marks the unique site of SL RNA transcription (Dossin & Schenkman, 2005). Following SRα silencing, tSNAP42 was not concentrated as this dot, but was spread throughout the nucleus. tSNAP42 might be the target to receive the signal to shut off SL RNA transcription, by losing its ability to bind to the SL RNA promoter. Indeed, a chromatin immunoprecipitation (ChIP) assay carried out on DNA from cells before and after silencing, indicated that the SL RNA transcription complex was not formed in SRα-depleted cells, as no binding of tSNAP42 to the SL RNA promoter was observed, as in uninduced cells. The specificity of binding to the SL RNA promoter was controlled by a lack of binding to the rRNA (Fig 3C). To examine whether the mRNA reduction observed in the SRα-silenced cells was entirely correlated with SL RNA shut-off, the tSNAP42 expression was silenced by RNAi. The results (supplementary Fig S3 online) show that in tSNAP42-silenced cells, SL RNA and mRNA levels were proportionally reduced.

Figure 3
Inhibition of spliced-leader RNA synthesis is linked to changes in the tSNAP complex. (A) The level of tSNAP proteins during SRP receptor α silencing. A 50 μg portion of proteins from induced (+Tet) and uninduced (−Tet) ...

To control for off-target silencing of SRα, we constructed a second stem–loop construct to a different domain of the gene. The results (supplementary Fig S4 online) show that this silencing has the same phenotype such as SL RNA reduction, tSNAP42 accumulation and aberrant localization. As the regulation observed in this study affected mostly SL RNA transcription, we termed this novel process spliced-leader RNA silencing (SLS).

Inducers of SLS

To investigate the source of the signal that elicited SLS, we examined the status of SRP on ribosomes. Release of the SRP–RNC complex requires GTPase activity, which in turn requires the interaction of SRP54–SRα; therefore, it is possible that the absence of the SRα receptor might cause the SRP–RNC complex to become immobilized. Accordingly, SRP might become fixed onto ribosomes. Extracts were prepared to separate free SRP present in the post-ribosomal supernatant (PRS) from ribosomal-bound SRP. Figure 4A shows that during silencing, the level of ribosomal-bound SRP, as detected by northern analysis of 7SL RNA, increased from 22%±4 in the uninduced cells to 65%±3 in the silenced cells. The quantity and quality of ribosomes in each fraction was determined using the 5.8S rRNA probe. These results indicate that, in the absence of the SRα receptor, SRP bound to ribosomes is not transient and that the interaction of SRP–RNC is stable and can withstand high-salt extraction. We examined next the localization of these SRP–RNC complexes in the cell by in situ hybridization with 7SL RNA. The results indicate that during silencing, all the SRP that are normally spread in the cytoplasm became concentrated in a defined area (Fig 4B). These data indicate that in SLS the signal is transmitted from the SRP stalled on the ribosomes to the nucleus to affect SL RNA transcription.

Figure 4
Changes in signal-recognition particle localization and ribosome association during signal-recognition parcticle receptor α depletion. (A) Binding of SRP to the RNC. (a) Extracts, PRS and ribosome fractions were prepared as described in the Methods ...

To investigate whether SLS can be induced under physiological conditions, parasites were exposed to low pH and the level of SL RNA was examined. The results show a significant reduction in SL RNA (Fig 5Aa) and mRNAs (Fig 5Ab). The reduction is attributed to SLS, as elevation in tSNAP42 was observed (Fig 5B), indicating that SLS is induced by the parasite in response to a relevant physiological stress.

Figure 5
Spliced-leader RNA silencing is activated under pH stress. (A) SL RNA and mRNA reduction under low pH. (a) RNA was prepared from parental strain cells grown for 2–3 days in pH 7.0 or 5.5 and subjected to northern analysis with SL RNA probe and ...

Discussion

In this study, we describe a novel regulatory circuit that responds to stress by the elimination of mRNA production, elicited by shut-off of SL RNA transcription. This is an immediate and extreme response, which leads to parasite death.

SLS was induced by a major stress to the protein translocation machinery; this is unlikely to occur in nature but might mimic other stresses that the parasite encounters. This indicates that the signalling pathway leading to SLS might be induced by different cues. At present, the signalling pathway involved in SLS is unknown. It is also unknown whether the inability of tSNAP42 to bind to its promoter is because it is modified directly by SLS or if SLS affects yet another factor that does not allow tSNAP42 binding.

SLS is activated under low pH, and at present we are investigating the effect of various stresses on SLS induction. The ability to transmit defects in sorting and folding of proteins to shut off a cellular process, such as transcription observed in this study, resembles UPR found in other eukaryotic cells. The UPR elicited by various stresses induces upregulation of the folding capacity of the endoplasmic reticulum and downregulation of the biosynthetic load, by shutting off protein synthesis at the transcriptional and translational levels (Schroder & Kaufman, 2005). Trypanosomes might carry two lines of defence against stress: a UPR-like mechanism and SLS. These pathways might be activated, depending on the severity of stress. Severe stress might activate SLS to quickly eliminate these ‘sick' parasites from the population.

It is interesting to examine whether the phenomenon described here is specific to trypanosomes, or whether similar transcription shut-off can be induced in metazoa on depletion of the SRP receptor. This is the first demonstration that SL RNA expression by SLS is a master regulator in the trypanosome cell. This discovery opens a new path towards a therapy based on SLS pathway activation. This could lead to stymied parasitic growth, and to relief from the devastating diseases caused by these parasites.

Methods

Cell growth, transfection and extract preparation. Procyclic T. brucei strain 29-13 (Wirtz et al, 1999) were grown in SDM-79 medium and transfected as described by Liu et al (2002). Extracts and preparation of ribosome and post-ribosomal supernatant have been described by Ben Shlomo et al (1999). Nuclear extracts were prepared as described previously (Das et al, 2005).

RNA analysis. Northern analysis for mRNA has been described by Liu et al (2002). Small RNAs were fractionated on a 10% (w/v) polyacrylamide gel containing 7 M urea. The RNA blots were hybridized to oligonucleotides or random primer DNA probes as specified in the supplementary Table I online. Primer extension was as described by Liu et al (2003).

Microscopy. Immunofluorescence was as described by Liu et al (2002). Cell nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI) for 5 min. For in situ hybridization, digoxigenin-labelled DNA probes were used as described previously (Lustig et al, 2005). Cells were visualized using a Zeiss LSM 510 META inverted microscope (Carl Zeiss AG 73446, Oberkochen, Germany). SEM was carried out as described by Rothmann et al (2000).

Cell permeabilization. The procedure was similar to that described by Tschudi & Ullu (1990). The only deviation from the published protocol is that transcription buffer TB × 1 was used (150 mM sucrose, 20 mM potassium, L-glutamate (Sigma, St Louis, MO, USA), 10 mM HEPES-KOH (pH 7.9), 2.5 mM MgCl2, 1 mM dithiotheritol, 10 μg/ml leupeptin).

For slot-blot analysis of the RNA synthesized in permeable cells, plasmid DNA or PCR products were used. Hybridization was carried out at 55–60°C in 60% (v/v) formamide, 2 × SSC (0.3 M sodium chloride, 0.03 M sodium citrate), 100 μg/ml salmon sperm DNA, 0.1% (w/v) Sarcosyl, with the entire RNA fraction extracted from permeable cells. After hybridization, filters were washed twice in 2 × SSC and 0.1% (w/v) SDS at 65°C for 30 min.

Chromatin immunoprecipitation. ChIP was carried out as described by Lowell & Cross (2004). Immunoprecipitated material after cross link reversal was deproteinized and subjected to PCR analysis using primers (see the supplementary information online). Supplementary information is available at EMBO reports online (http://www.emboreports.org).

Supplementary Material

Supplementary Information

Acknowledgments

We thank M. Mandelboim for her help in the permeable cell expression. This study was supported by an International Research Scholar grant from the Howard Hughes Research Institute to S.M.

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