• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Hum Immunol. Author manuscript; available in PMC Apr 12, 2007.
Published in final edited form as:
PMCID: PMC1851905


Robert J. Freishtat, MD, MPH
Robert J. Freishtat, Division of Emergency Medicine and Research Center for Genetic Medicine, Children’s National Medical Center, Department of Pediatrics, George Washington University School of Medicine and Health Sciences, Washington, DC;
Lindsay W. Mitchell, BS
Lindsay W. Mitchell, Research Center for Genetic Medicine, Children’s National Medical Center, Washington, DC;
Svetlana D. Ghimbovschi, MD, PhD
Svetlana D. Ghimbovschi, Research Center for Genetic Medicine, Children’s National Medical Center, Washington, DC;
Samuel B. Meyers
Samuel B. Meyers, College of Letters and Sciences, University of California, Davis, Davis, CA;


NKG2A is commonly expressed on cytotoxic cells but has been found on activated T-Helper (TH) cells. In identifying novel differentiating markers between TH1 and TH2 lymphocytes, we focused on NKG2A expression. TH1 and TH2 cells were negatively isolated from healthy volunteers for microarray analysis and RT-PCR. Flow cytometry of quiescent and activated TH1 and TH2 cells was performed. Isolates were >95% pure CD3+CD4+ cells (TH1=90.3% and TH2=84.1%). Microarrays showed differential expression of NKG2A and NKG2C isoforms between TH1 and TH2 cells. RT-PCR showed greater expression of NKG2A in TH2 cells (4-fold) and NKG2C in TH1 cells (3-fold). Flow studies showed tripling of TH2 NKG2A with activation to 10.76±4.01% (p=0.05), a 23-fold increase in CD56 to 35±14.54% (p=0.03), and an increase in NKG2A+CD56+ double-positive cells to 3.04±1.38% (p=0.04). TH1 lymphocytes did not differ with activation. We identified co-induction of NKG2A and CD56 upon activation of TH2 cells. These cells would likely bind more HLA-E and show increased effector inhibition. Given that certain viruses are known to decrease MHC class I and thus HLA-E production by antigen presenting cells, activated TH2 cells would bind less HLA-E in this scenario. This would likely result in less effector inhibition and a relatively robust TH2 response.

Keywords: TH1 Cells, TH2 Cells, Natural Killer Cells, CD56, Lymphocyte Activation
Abbreviations: NK — Natural Killer Cell, HLA — Human Leukocyte Antigen, TH — T-Helper Cell, IL — Interleukin, IFN — Interferon, TCR — T Cell Receptor, PCR — Polymerase Chain Reaction, MHC — Major Histocompatibility Complex, PBMC — Peripheral Blood Mononuclear Cell


The NKG2A receptor is a C-type lectin found primarily on cytotoxic cells such as natural killer (NK) cells and CD8+ T lymphocytes [1, 2, 3, 4]. The binding of NKG2A to its natural ligand, human non-classical class I leukocyte antigen (HLA)–E [5, 6, 7, 8, 9, 10], induces its immunoreceptor tyrosine-based inhibition motif (ITIM) and suppresses cytotoxic cell effector activity [11, 12, 13, 14]. The inhibitory capability of NKG2A on a bearing cell remains relatively stable over time. This is due to a constant supply of fresh NKG2A receptors from endosomal recycling between the plasma membrane and intracellular compartments [15]. However, NKG2A receptor turnover and therefore surface expression can be altered by extracellular mechanisms. For example, NK cell surface expression of NKG2A is increased in the presence of interleukin (IL) -15 [16].

In addition to its familiar cytotoxic cell expression, NKG2A has recently been discovered on human CD4+CD8-T-Helper (TH) lymphocytes by way of CD3-mediated activation with IL-10 and transforming growth factor (TGF)–β[17]. In addition, non-activated murine Th cells polarized in vitro to the Th1 phenotype were found to bind a single antibody for NKG2A and two other C-type lectin family members [18].

Following from these discoveries, multiple reports emphasize the emerging importance of NKG2A in the cytotoxic response to viruses [19, 20, 21, 22], cancer [23, 24], and HIV [25, 26]. However, the role of NKG2A on non-cytotoxic cells, like TH cells, and the potential for differential expression between TH1 and TH2 subsets remains unclear. Therefore we report the negative isolation of TH1 and TH2 lymphocytes with a novel antibody cocktail developed by the authors. We followed this with mRNA profiling, quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR), and protein expression studies using flow cytometry. We found moderate levels of NKG2A expression on quiescent TH1 and TH2 cells but only low levels of NKG2C on either subtype. Interestingly, after four hours of phorbol-mediated activation, we found significantly increased TH2 cell expression of NKG2A, CD56, and NKG2A-CD56 double-positivity. This represents the first description of MHC class I recognition capability by TH2 cells as well as raises the possibility of a role for NKG2A in the regulation of TH2 cell effector functions.

Materials and Methods


Eleven apparently healthy, non-atopic, non-asthmatic volunteers between the ages of 18 and 50 years had 30 mL of venous blood drawn directly into ethylenediaminetetraacetic acid (EDTA)-containing vials. The participants were representative of both genders and a diverse group of ethnicities and races. The investigation was approved by the Institutional Review Board and General Clinical Research Center (GCRC) Advisory Committee and performed in the Children’s National Medical Center GCRC.

Cell Isolation

Cell separation procedures were initiated within 30 minutes of blood collection. Whole blood was emptied into conical tubes and centrifuged at low speed. Platelet-rich plasma was removed and the remaining blood concentrate was diluted to 30 mL total volume with 2% fetal bovine serum (FBS) in 1X phosphate-buffered saline (PBS) then centrifuged over Ficoll Paque PLUS™ (Amersham Biosciences, Piscataway, NJ) density medium to isolate the peripheral blood mononuclear cell (PBMC) layer.

TH1 and TH2 Lymphocyte Isolation

After washing and resuspension of the PBMC layer in PBS+2%FBS, the sample was split into three equal aliquots by volume. Cells were counted by hemocytometer to assure a concentration less than 8 × 107 cells/mL. From each aliquot, either non-differentiated TH cells, TH1 or TH2 lymphocytes were negatively isolated using StemSep™ (StemCell Technologies, Vancouver, BC).

Isolations were conducted according to the manufacturer’s protocol except for the addition of novel combinations of monoclonal antibodies we developed and validated for the negative isolation of TH1 and TH2 lymphocytes. For the negative isolation of TH1 cells, approximately 5 × 106 cells were treated for 10 minutes at 4ºC with anti-CRTH2-biotin (Clone BM16; Miltenyi Biotec, Auburn, CA) to bind TH2 cells. After washing once, cells were incubated at room temperature for 15 minutes with anti-biotin tetramers (StemCell) and cocktail containing antibodies to CD8, CD14, CD16, CD19, CD56, CCR4, and glycophorin A. Samples were centrifuged, the supernatant removed, and the cell pellets containing antibody bound PBMCs and unbound TH1 cells were suspended in PBS+2%FBS. Following a final incubation for 15 minutes at room temperature with StemCell magnetic colloid, samples were passed through a magnetic column. Eluted TH1 cell isolates were immediately prepared for RNA isolation and/or flow cytometry.

The negative isolation of TH2 cells was similar, except samples were first treated with anti-CD29 (β1 integrin)-biotin (4B7R; Research Diagnostics Inc., Flanders, NJ) to bind TH1 cells at 4ºC for 10 minutes. Samples were washed and then incubated for 15 minutes at room temperature with an antibody cocktail containing anti-CD8, anti-CD14, anti-CD16, anti-CD19, anti-CD56, anti-CXCR3, and anti-glycophorin A. The remaining isolation procedures were identical to those for TH1 cells.

mRNA Expression Profiling

Three pairs of quiescent TH1 and TH2 samples were used for mRNA expression profiling using PicoPure™ (Arcturus Bioscience, Mountain View, CA) RNA extraction methods. Total RNA yield was determined by spectrophotometer UV absorbtion and RNA quality by LabChip® Bioanalyzer electrophoresis. RiboAmp® (Arcturus) amplification was performed for two-rounds and BioArray® (Enzo Life Sciences, Farmingdale, NY) biotin labeling was then performed according the manufacturer’s standard protocols.

Expression profiling data was obtained for each sample individually using the Affymetrix (Santa Clara, CA) U133A GeneChip® (22,283 probe sets querying ~18,000 genes), with standard operating procedures and quality controls as we have published [27, 28]. Expression values were calculated using both DNA-Chip Analyzer 1.3 (dChip MBE/mismatch model) [www.dchip.org] and Microarray Suite 5 (MAS5) (Affymetrix) probe set algorithms. Comparative analyses of dChip and MAS5 expression values were performed in GeneSpring® 7 (Agilent Technologies, Palo Alto, CA). Expression values were filtered for probe set present calls in at least two out of the three TH1 and TH2 cell samples. ANOVA (1-way) analyses with no multiple testing corrections and a p-value cutoff of 0.05 were used to further filter the expression values. Data interpretation was limited to filtered probe sets containing NKG* or KLR* (i.e. C-type lectins) in their primary or alternate names. Probe sets ultimately meeting all filter criteria were examined to determine candidates with consistent differential mRNA expression between TH1 and TH2 cells according to both dChip- and MAS5-derived results. We have previously shown such comparative analyses to be effective in reducing the number of false positives obtained from microarray studies [28].

Quantitative Real-Time Reverse-Transcriptase PCR

RNA was extracted by Trizol®/chloroform phase separation for RT-PCR. Total RNA quantities were verified by UV absorption at 260 nm and integrities by 18s and 28s ribosomal bands using LabChip® analysis on a 2100 Bioanalyzer (Agilent, Palo Alto, CA). SuperScript™ III First-Strand Synthesis System (Invitrogen, Carlsbad, CA) was used for cDNA synthesis.

T-bet:GATA-3 mRNA expression ratios were used to confirm the enrichment for TH1 and TH2 cell fractions as adapted from methods described by Chakir, et al. [29, 30]. Briefly, DNA primers were designed according to known gene sequences as follows: T-bet (Forward) 5′-CAA CGC CCG GCT GCA TAT CG[FAM]T G-3′; T-bet (Reverse) 5′-TGG TAG GCA GTC ACG GCA AT-3′; GATA-3 (Forward) 5′-CAG AGG ACC CTG TCT GCA ATG CCT G[FAM]G-3′; GATA-3 (Reverse) 5′-TCC TCC AGT GAG TCA TGC ACT TT-3′. T-bet and GATA-3 PCR samples were run in multiplex and results were calculated using the Ct method [31]. A formula for the purity of TH1 cells in a given sample was derived [(Pu=R/(R+1), where Pu=TH1 purity and R=T-bet:GATA-3 ratio] and used to calculate the enrichment for TH1 and TH2 cells in each sample.

Additional primers were designed for human NKG2A and NKG2C as well as a primer set designed to mimic the single Affymetrix Probe Set (PS) 206785_s_at recognizing both transcripts. Original DNA primers for this expression profile validation step were designed according to known gene sequences as follows: NKG2A (Forward) 5′-CTA CTC AGG GGC AGA TTC AGG TCT GAG [FAM]AG-3′; NKG2A (Reverse) 5′-TGG CCT CTC CAC TAA AGG ATG-3′; NKG2C (Forward) 5′-GAA TCA TCA TCA GGA CAA TGC AAA TGA [FAM]TC-3′; NKG2C (Reverse) 5′-GCC AAG GTT TAC TGC CAC CT-3′; Probe Set 206785_s_at (Forward) 5′- GAG CCA TCC TAG CAT TTG TGT CGG GC[FAM]C-3′; Probe Set 206785_s_at (Reverse) 5′-CCC TCA GAC ATT GGC AAC CA-3′. NKG2A, NKG2C, and Probe Set PCR samples were run using the Standard Curve Method [31].

All PCR reactions were completed on quiescent cells with cycles of 95°C, 55°C, and 72°C. JOE-labeled human TATA-binding protein (TBP) (Invitrogen)-containing wells served as positive controls and polymerase-free wells as negative controls. Reactions were run using an ABI PRISM® 7700 PCR instrument (Applied Biosystems, Foster City, CA) and relative gene expression levels were calculated using Sequence Detection System Software (ABI). Amplification curves reaching threshold after 40 cycles were excluded from analysis. Standard errors of means were calculated using the “Delta Method” [32]. Gel electrophoresis was used to confirm appropriate amplicon sizes of PCR products and absence of primer-dimer contamination.

Flow Cytometry

TH1 and TH2 cell isolates from eight subjects were halved. One half of each cell isolate was activated for 4 hours with 50 μg/μL of phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO) in the presence of 1 μg/μL of ionomycin (Sigma) and 1 μg/μL of brefeldin A (Sigma).

Non-activated and activated cells underwent a series of incubations with fluorescent-labeled monoclonal antibodies, as well as permeablization where appropriate, and then fixation with 1% paraformaldehyde. Four-color flow cytometry (FACSCalibur™ System, BD) was performed with varying combinations of anti-CD3-PerCP (BD), anti-CD4-FITC (BD), anti-CD4-APC (BD), anti-CD8-FITC (BD), anti-CD29-biotin (RDI), anti-CD56-APC (BD), anti-CXCR3-APC (BD), anti-CRTH2-PE (Miltenyi), anti-CCR4-PE (BD), anti-NKG2A-APC (R&D Systems, Minneapolis, MN), anti-NKG2C-PE (R&D Systems), anti-interferon (IFN) –γ (BD), anti-interleukin (IL) -4 (BD), and anti-Biotin-APC (BD) using appropriate isotype and negative (unlabeled cells) controls. Cells were gated for TH lymphocytes using forward- and side-scatter properties and CD3+CD4+ double positivity. Data were analyzed with FlowJo 5.7 (Tree Star, Inc., Ashland, OR) to confirm the TH1 and TH2 isolations as well as to determine the frequency of NKG2A marker positivity among cells in the isolated TH fractions. Statistical significance of percent positive cells and mean fluorescence intensity between cell types was tested with SPSS 13 (SPSS) using paired T-tests of log-transformed data.


Negative Isolation of TH1 and TH2 Lymphocytes and Fraction Purity

Validation of the negative isolation cocktails was performed both by flow cytometry and RT-PCR on a select group of samples. Flow cytometry showed that TH1 and TH2 cell isolates were depleted of cells positive for markers contained in the isolation cocktails. (Figure 2) Further, the TH1 and TH2 cell enrichments were characterized using RT-PCR as in Chakir, et al. [29]. This method utilizes the ratio of T-bet:GATA-3 mRNAs to determine an estimate of the TH1:TH2 ratio within each isolate The mean T-bet:GATA-3 ratio for TH1 lymphocytes was 9.3±6.28 (PuTH1=90.3%) and for TH2 cells was 0.19±0.06 (PuTH2=84.1%). The mean T-bet:GATA-3 ratio for TH2 cells was statistically significantly different from that for a group of non-fractionated CD4+ TH-enriched control cells (2.39±1.28, p=0.04) (Figure 1). The TH1 cells were not statistically different from the non-fractionated TH cells or the TH2 cells.

Figure 1
Comparison of ratios of mRNA for T-bet to GATA-3, and T-bet and GATA-3 to TBP, in TH1 and TH2 lymphocyte subsets and non-fractionated CD4+ T lymphocytes by quantitative RT-PCR. (*p=0.04)
Figure 2
Flow cytometry of a representative sample of lymphocytes before and after enrichment for TH1 and TH2 lymphocytes. All analyses were gated by forward- and side-scatter properties to select for lymphocytes. TH1 and TH2 cell analyses were also gated on CD3+CD4+ ...

TH1 and TH2 mRNA Expression Profiles

mRNA expression profiles were completed on each sample of TH1 and TH2 cells from three of the eleven subjects using Affymetrix U133A microarrays in order to determine differential gene expression between the cell types. Comparative analyses using both dChip- and MAS5-calculated expression values were performed in GeneSpring®, generating expression values that were filtered for present calls and a p-value of 0.05.

A list of 46 TH1:TH2 cell differentially expressed transcripts was generated by these analyses (Table 1). Of these, two contained NKG* or KLR* in their names: 1) Combined NKG2A and NKG2C [TH1:TH2 Relative expression ratio (Range) =7.69 (6.16 to 20.99), p=0.02 by dChip] and 2) KLRK1 [1.46 (1.44 to 1.53), p=0.04 by dChip] (Table 2). NKG2A, NKG2C, and KLRK1 have proximate chromosomal location (chromosome 12p13.2–13.1) and all similarly bind HLA-E. However, NKG2A and NKG2C were chosen as targets for RT-PCR confirmation because the single probe set representing both NKG2A and NKG2C showed the greatest difference in relative expression between the cell types and these markers were found previously on CD4+ TH cells [17].

Table 1
Relative expression of differentially-expressed transcripts and T-bet and GATA-3 as determined by microarray analysis for quiescent TH1 and TH2 cells
Table 2
Relative Expression of C-type Lectin Genes in TH1 with Respect to TH2 Lymphocytes by Microarray and Quantitative RT-PCR

NKG2 Isoform mRNA Expression

We extended the microarray data by quantitative RT-PCR using primers specific for NKG2A and NKG2C, as well as a primer set recognizing both isoforms. This latter primer set corresponds to the region of the transcript also queried by Affymetrix Probe Set (PS) 206785_s_at. A paired analysis of TH1 and TH2 cells from the same three microarray-studied subjects was performed. Wells showing detectable primer-dimers by gel electrophoresis were excluded. Standard curves allowed the calculation of relative expression levels (TH1:TH2) as follows: NKG2A = 0.24±0.18 (~4-fold enrichment in TH2) and NKG2C = 2.83±1.31 (~3-fold enrichment in TH1). The primer set corresponding to Affymetrix Probe Set (PS) 206785_s_at showed a ratio of 0.60±0.35 (~2-fold enrichment in TH2). (Table 2)

Differential NKG2 Protein Expression after Activation of TH2 Cells

To validate the RT-PCR finding of NKG2A predominance among TH2 cells, flow cytometry was carried out for both TH1 and TH2 cells before and after cell activation in samples from the remaining eight subjects. Samples were gated on CD3+CD4+ cells during analysis. However, no cell isolates showed evidence of CD8+ cell contamination. (Figure 2)

TH2-enriched lymphocytes showed Mean±SEM NKG2A+ surface expression of 2.89±0.78%, CD56+ of 1.43±0.91%, NKG2C+ of 0.56±0.14%, and NKG2A+CD56+ cells of 0.16±0.09%. Once activated, the TH2 lymphocytes showed a significant increase in the number of cells positive for NKG2A, CD56, and for both. TH2 cells expressing NKG2A tripled to 10.76±4.01% (p=0.05); CD56 expression showed a 23-fold increase to 35±14.54% (p=0.03); and NKG2A+CD56+ double-positive cells increased significantly to 3.04±1.38% (p=0.04). NKG2C expression did not change significantly: 0.72±0.39% (p=NS). (Table 3 and Figure 3)

Figure 3
Flow cytometry comparison for a representative pair of TH1 and TH2 lymphocytes before and after activation examining NKG2A, intracellular IFN-γ, and intracellular IL-4 positivity. These analyses were gated to select for CD3+CD4+ cells only.
Table 3
Surface Antigen and Intracellular Cytokine Expression by Cell Type and Activation State

Before activation, the TH1-enriched lymphocytes showed NKG2A+ of 2.56±0.52%, CD56+ of 3.15±1.69%, NKG2C+ of 1.12±0.42%, and NKG2A+CD56+ cells of 0.86±0.5%. Activated TH1 lymphocytes showed no statistically significant change in mean surface expression of NKG2A+ (4.66±1.59%; p=0.08), CD56+ (7.27±4.77%; p=NS), NKG2C+ (1.59±0.52%; p=NS), and NKG2A+CD56+ (1.38±1.08%; p=NS) (Table 3 and Figure 2).

Although there were increases in IFN-γ expression in both activated TH1 and TH2 cell samples, these changes from the non-activated state were not significant. However, there were significant increases in intracellular IL-4 in both activated samples, especially TH2 cells. (Table 3 and Figure 3).


Studies that examine TH1 and TH2 lymphocytes are fundamental to answering questions about the balance of these cells and their cytokines in diseases such as asthma and many autoimmune states. However, TH cells polarized in culture are likely not ideal for these studies because they may not display the same in vitro phenotype as do in vivo TH1 and TH2 cells. Therefore, we have reported the first ex vivo analyses of negatively isolated TH1 and TH2 cells by microarray, RT-PCR, and flow cytometry. We focused data analysis and validation on NKG2A to determine if it has differential expression between TH1 and TH2 cells.

Using a novel antibody cocktail we developed for the negative isolation of human TH1 and TH2 cells, we obtained 90.3% enrichment for TH1 cells and 84.1% enrichment for TH2 cells. These results were validated with the methods of Chakir, et al. [29] using the T-bet:GATA-3 ratios of each sample by RT-PCR. By flow cytometry, we found a three-fold increase in NKG2A surface protein expression following activation on TH2 cells, though not on TH1 cells. The NKG2C receptor surface expression on TH2 cells did not change noticeably under the same conditions.

The antibody combinations used for the negative isolations were chosen based on differential prevalence of surface markers on each cell type reported by Matsui, et al. and in other published studies [33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46]. We recognized a priori this would not achieve 100% purity, but would allow for sufficient purity and yields to conduct these experiments. Additionally, some cells would not be expected to express any of the markers in the isolation cocktails and therefore would appear in both cell type enrichments. These cells could have comprised up to 15% of the total CD3+CD4+ cell counts [33] and therefore could impact the results, but their presence in both samples would bias results towards the null.

Additionally, a small percentage (1–5%) of the isolated cells was CD3+CD4−. These were not evaluated more closely but could represent NKT cells as well as other lymphocyte subsets. These cells could have skewed the microarray and PCR results to some degree as NKT cells, in particular, highly express NKG2A and NKG2C. However, in the flow cytometry studies we conducted, we gated on CD3+CD4+ cells only, so it is not possible for NKT cells to have affected those results. This could explain the discrepancy between the RT-PCR and flow cytometry results for quiescent cells.

As well, there were some CD56+ cells among the non-activated cells of both types which could represent actual contamination or mild ex vivo activation during cell processing. In either event, the longitudinal nature of the study examined intra-individual differences and this small percentage of CD56+ cells would not have had an effect on the results. Despite each of these limitations, the use of human ex vivo cells made this an attractive approach.

In light of the earlier finding that activated non-fractionated CD4+ TH lymphocytes express NKG2A [17], our work indicates that the TH2 fraction is responsible for this. Given a previous study that found NKG2A, NKG2C, or NKG2E on murine Th1 cells [18], one might have expected the human TH1 cells to preferentially express this receptor. However, as we did find low levels of NKG2A and NKG2C expression on TH1 cells, our results are not incongruous with that prior study.

Of note, we also found marked inter-individual variability in the level of expression of the markers of interest before and after activation. This phenomenon has been described for NKG2 receptors in rhesus monkeys previously [47] and could reflect the dynamic nature of these receptors as their surface expression switches from one type to another.

It is clear from our data that NKG2A receptors, which are MHC class I-responsive via HLA-E, undergo increased surface expression on TH2 lymphocytes following activation. However, this is a departure from standard as the TH2 phenotype customarily promotes a humoral immune response to foreign antigen recognition by the MHC class II complex. TH1 cells are usually responsible for instigating a cellular immune response to foreign antigen presentation by MHC class I to the T-cell receptor (TCR) complex. As such, this is the first report of TH2 cell capability for MHC class I recognition.

There are published data that provide context for our findings. Marusina, et al. showed that GATA-3 is a key transcription factor in the regulation of NKG2A expression [48]. In fact, GATA-3 up-regulation also initiates TH2-specific cell polarization via IL-4 induction of STAT6 [49]. GATA-3 acts at the CNS-I region of the Th2 cytokine coding locus to activate transcription of IL-4 and IL-13 [50, 51, 52, 53, 54, 55, 56]. At a certain point, GATA-3 auto-activates its own transcription leading to further transcription of Th2 cytokines and the commitment of naïve T Helper cells to the TH2 phenotype [57, 58]. Further, Romero, et al. showed that that the potent TH2 cytokine, IL-10, is an important positive contributor to NKG2A expression on activated CD4+ TH cells [17].

The discovery of NKG2A, an inhibitory receptor for MHC class I peptides via HLA-E, on TH2 cells raises questions as to its function. It seems plausible that the NKG2A receptor could act as an inhibitory mechanism for modulation of TH2 cell function. If NKG2A behavior on TH2 cells is analogous to that on CD8+ T lymphocytes, one would expect concomitant binding of NKG2A to HLA-E along with the CD4+-TCR to MHC class II. This parallel receptor-ligand binding would hypothetically result in transmission of an inhibitory signal from NKG2A along with the activating signal from the TCR. In this way, NKG2A would serve to inhibit typical TH2 effector functions by dampening the activating signal from the TCR. For example, the co-regulation of CD56 with NKG2A identified in our TH2 samples indicates that NKG2A receptor surface expression correlates with degree of cell activation. This suggests that in the situation of viral infections where lymphocytes are activated but MHC class I production is often decreased [59, 60], NKG2A inhibition of TH2 effector function would be diminished despite an increased level of surface expression. This hypothesis would lead one to expect a relatively robust TH2 response compared to that of activated TH1 cells, in light of their lack of NKG2A expression.

In fact, a successful attempt to regulate inflammation via the NKG2A receptor was recently reported by Kawamura, et al. In their study, anti-NKG2A monoclonal antibodies were shown to restrict donor T cell expansion and improve signs of murine acute graft-versus-host disease [61]. However, it has not been shown whether these results can be extrapolated to humans and other diseases where TH1/TH2 cell and cytokine balance is important.

Our identification of NKG2A receptors on activated human TH2 lymphocytes is the next step in this extrapolation. Given the context provided by recent reports of NKG2A on CD4+ TH cells, our findings raise important functional questions about its role in TH1/TH2 cell and cytokine balance. The future study of TH1 and TH2 cells in autoimmune states and acute and chronic TH-related diseases will benefit from clarifying the functional significance of activated TH2 cell NKG2A receptors in humans.


The author’s would like to thank Kanneboyina Nagaraju, PhD for his thoughtful advice during the preparation of this manuscript. This study was supported by funding from the National Institutes of Health (Grants K12-HD-01399 and M01-RR-020359), the Frank and Nancy Parsons Foundation, and a Gordon C. Avery Award from Children’s National Medical Center, Washington, DC.


1. Houchins JP, Yabe T, McSherry C, Bach FH. DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 1991;173(4):1017. [PMC free article] [PubMed]
2. Hofer E, Duchler M, Fuad SA, Houchins JP, Yabe T, Bach FH. Candidate natural killer cell receptors. Immunol Today. 1992;13(11):429. [PubMed]
3. Yabe T, McSherry C, Bach FH, Fisch P, Schall RP, Sondel PM, Houchins JP. A multigene family on human chromosome 12 encodes natural killer-cell lectins. Immunogenetics. 1993;37(6):455. [PubMed]
4. Lazetic S, Chang C, Houchins JP, Lanier LL, Phillips JH. Human natural killer cell receptors involved in MHC class I recognition are disulfide-linked heterodimers of CD94 and NKG2 subunits. J Immunol. 1996;157(11):4741. [PubMed]
5. Posch PE, Borrego F, Brooks AG, Coligan JE. HLA-E is the ligand for the natural killer cell CD94/NKG2 receptors. J Biomed Sci. 1998;5(5):321. [PubMed]
6. Braud VM, Allan DS, O’Callaghan CA, Soderstrom K, D’Andrea A, Ogg GS, Lazetic S, Young NT, Bell JI, Phillips JH, Lanier LL, McMichael AJ. HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C. Nature. 1998;391(6669):795. [PubMed]
7. Brooks AG, Borrego F, Posch PE, Patamawenu A, Scorzelli CJ, Ulbrecht M, Weiss EH, Coligan JE. Specific recognition of HLA-E, but not classical, HLA class I molecules by soluble CD94/NKG2A and NK cells. J Immunol. 1999;162(1):305. [PubMed]
8. Lee N, Llano M, Carretero M, Ishitani A, Navarro F, Lopez-Botet M, Geraghty DE. HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. 1998;95(9):5199. [PMC free article] [PubMed]
9. Maier S, Grzeschik M, Weiss EH, Ulbrecht M. Implications of HLA-E allele expression and different HLA-E ligand diversity for the regulation of NK cells. Hum Immunol. 2000;61(11):1059. [PubMed]
10. Miller JD, Weber DA, Ibegbu C, Pohl J, Altman JD, Jensen PE. Analysis of HLA-E peptide-binding specificity and contact residues in bound peptide required for recognition by CD94/NKG2. J Immunol. 2003;171(3):1369. [PubMed]
11. Houchins JP, Lanier LL, Niemi EC, Phillips JH, Ryan JC. Natural killer cell cytolytic activity is inhibited by NKG2-A and activated by NKG2-C. J Immunol. 1997;158(8):3603. [PubMed]
12. Borrego F, Kabat J, Sanni TB, Coligan JE. NK cell CD94/NKG2A inhibitory receptors are internalized and recycle independently of inhibitory signaling processes. J Immunol. 2002;169(11):6102. [PubMed]
13. Braud VM, Aldemir H, Breart B, Ferlin WG. Expression of CD94-NKG2A inhibitory receptor is restricted to a subset of CD8+ T cells. Trends Immunol. 2003;24(4):162. [PubMed]
14. Kabat J, Borrego F, Brooks A, Coligan JE. Role that each NKG2A immunoreceptor tyrosine-based inhibitory motif plays in mediating the human CD94/NKG2A inhibitory signal. J Immunol. 2002;169(4):1948. [PubMed]
15. Borrego F, Masilamani M, Kabat J, Sanni TB, Coligan JE. The cell biology of the human natural killer cell CD94/NKG2A inhibitory receptor. Mol Immunol. 2005;42(4):485. [PubMed]
16. Mingari MC, Vitale C, Cantoni C, Bellomo R, Ponte M, Schiavetti F, Bertone S, Moretta A, Moretta L. Interleukin-15-induced maturation of human natural killer cells from early thymic precursors: selective expression of CD94/NKG2-A as the only HLA class I-specific inhibitory receptor. Eur J Immunol. 1997;27(6):1374. [PubMed]
17. Romero P, Ortega C, Palma A, Molina IJ, Pena J, Santamaria M. Expression of CD94 and NKG2 molecules on human CD4(+) T cells in response to CD3-mediated stimulation. J Leukoc Biol. 2001;70(2):219. [PubMed]
18. Meyers JH, Ryu A, Monney L, Nguyen K, Greenfield EA, Freeman GJ, Kuchroo VK. Cutting edge: CD94/NKG2 is expressed on Th1 but not Th2 cells and costimulates Th1 effector functions. Journal of Immunology. 2002 Nov. 15;:5382. [PubMed]
19. Jinushi M, Takehara T, Tatsumi T, Kanto T, Miyagi T, Suzuki T, Kanazawa Y, Hiramatsu N, Hayashi N. Negative regulation of NK cell activities by inhibitory receptor CD94/NKG2A leads to altered NK cell-induced modulation of dendritic cell functions in chronic hepatitis C virus infection. J Immunol. 2004;173(10):6072. [PubMed]
20. Nattermann J, Nischalke HD, Hofmeister V, Ahlenstiel G, Zimmermann H, Leifeld L, Weiss EH, Sauerbruch T, Spengler U. The HLA-A2 restricted T cell epitope HCV core 35–44 stabilizes HLA-E expression and inhibits cytolysis mediated by natural killer cells. Am J Pathol. 2005;166(2):443. [PMC free article] [PubMed]
21. Gold MC, Munks MW, Wagner M, McMahon CW, Kelly A, Kavanagh DG, Slifka MK, Koszinowski UH, Raulet DH, Hill AB. Murine cytomegalovirus interference with antigen presentation has little effect on the size or the effector memory phenotype of the CD8 T cell response. J Immunol. 2004;172(11):6944. [PubMed]
22. Lopez-Botet M, Angulo A, Guma M. Natural killer cell receptors for major histocompatibility complex class I and related molecules in cytomegalovirus infection. Tissue Antigens. 2004;63(3):195. [PubMed]
23. Sheu BC, Chiou SH, Lin HH, Chow SN, Huang SC, Ho HN, Hsu SM. Up-regulation of inhibitory natural killer receptors CD94/NKG2A with suppressed intracellular perforin expression of tumor-infiltrating CD8+ T lymphocytes in human cervical carcinoma. Cancer Res. 2005;65(7):2921. [PubMed]
24. Vitale C, Chiossone L, Morreale G, Lanino E, Cottalasso F, Moretti S, Dini G, Moretta L, Mingari MC. Human natural killer cells undergoing in vivo differentiation after allogeneic bone marrow transplantation: analysis of the surface expression and function of activating NK receptors. Mol Immunol. 2005;42(4):405. [PubMed]
25. Wesch D, Kabelitz D. Differential expression of natural killer receptors on Vdelta1 gammadelta T cells in HIV-1-infected individuals. J Acquir Immune Defic Syndr. 2003;33(4):420. [PubMed]
26. Costa P, Rusconi S, Fogli M, Mavilio D, Murdaca G, Puppo F, Mingari MC, Galli M, Moretta L, De Maria A. Low expression of inhibitory natural killer receptors in CD8 cytotoxic T lymphocytes in long-term non-progressor HIV-1-infected patients. Aids. 2003;17(2):257. [PubMed]
27. Chen YW, Zhao P, Borup R, Hoffman EP. Expression profiling in the muscular dystrophies: identification of novel aspects of molecular pathophysiology. J Cell Biol. 2000;151(6):1321. [PMC free article] [PubMed]
28. Tumor Analysis Best Practices Working Group. Expression profiling - best practices for data generation and interpretation in clinical trials. Nat Rev Genet. 2004;5:229. [PubMed]
29. Chakir H, Wang H, Lefebvre DE, Webb J, Scott FW. T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3. J Immunol Methods. 2003;278(1–2):157. [PubMed]
30. Freishtat RJ, Diette GB, Moses L, Hoffman EP. Negative Isolation of Peripheral Blood TH2 Cells for Lung Injury Research. Pediatric Research. 2004;55:60A.
31. ABI Prism Sequence Detection System. User Bulletin #2. vol 2005. 1997.
32. van Kempen GM, van Vliet LJ. Mean and variance of ratio estimators used in fluorescence ratio imaging. Cytometry. 2000;39(4):300. [PubMed]
33. Matsui M, Araya S, Wang HY, Onai N, Matsushima K, Saida T. Circulating lymphocyte subsets linked to intracellular cytokine profiles in normal humans. Clin Exp Immunol. 2003;134(2):225. [PMC free article] [PubMed]
34. Wilczynski JR, Tchorzewski H, Banasik M, Glowacka E, Wieczorek A, Lewkowicz P, Malinowski A, Szpakowski M, Wilczynski J. Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia. Eur J Obstet Gynecol Reprod Biol. 2003;109(1):8. [PubMed]
35. Sasaki K, Tsuji T, Jinushi T, Matsuzaki J, Sato T, Chamoto K, Togashi Y, Koda T, Nishimura T. Differential regulation of VLA-2 expression on Th1 and Th2 cells: a novel marker for the classification of Th subsets. Int Immunol. 2003;15(6):701. [PubMed]
36. Michimata T, Tsuda H, Sakai M, Fujimura M, Nagata K, Nakamura M, Saito S. Accumulation of CRTH2-positive T-helper 2 and T-cytotoxic 2 cells at implantation sites of human decidua in a prostaglandin D(2)-mediated manner. Molecular Human Reproduction. 2002;8(2):181. [PubMed]
37. Croker BA, Handman E, Hayball JD, Baldwin TM, Voigt V, Cluse LA, Yang FC, Williams DA, Roberts AW. Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in T(H)1 responses. Immunol Cell Biol. 2002;80(3):231. [PubMed]
38. Colantonio L, Recalde H, Sinigaglia F, D’Ambrosio D. Modulation of chemokine receptor expression and chemotactic responsiveness during differentiation of human naive T cells into Th1 or Th2 cells. European Journal of Immunology. 2002;32(5):1264. [PubMed]
39. Campbell JJ, Brightling CE, Symon FA, Qin S, Murphy KE, Hodge M, Andrew DP, Wu L, Butcher EC, Wardlaw AJ. Expression of chemokine receptors by lung T cells from normal and asthmatic subjects. Journal of Immunology. 2001;166(4):2842. [PubMed]
40. Cosmi L, Annunziato F, Galli MIG, Maggi RME, Nagata K, Romagnani S. CRTH2 is the most reliable marker for the detection of circulating human type 2 Th and type 2 T cytotoxic cells in health and disease. European Journal of Immunology. 2000;30(10):2972. [PubMed]
41. Clissi B, D’Ambrosio D, Geginat J, Colantonio L, Morrot A, Freshney NW, Downward J, Sinigaglia F, Pardi R. Chemokines fail to up-regulate beta 1 integrin-dependent adhesion in human Th2 T lymphocytes. Journal of Immunology. 2000;164(6):3292. [PubMed]
42. Nagata K, Hirai H, Tanaka K, Ogawa K, Aso T, Sugamura K, Nakamura M, Takano S. CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s) FEBS Letters. 1999;459(2):195. [PubMed]
43. Jinquan T, Quan S, Feili G, Larsen CG, Thestrup-Pedersen K. Eotaxin activates T cells to chemotaxis and adhesion only if induced to express CCR3 by IL-2 together with IL-4. Journal of Immunology. 1999;162(7):4285. [PubMed]
44. Imai T, Nagira M, Takagi S, Kakizaki M, Nishimura M, Wang J, Gray PW, Matsushima K, Yoshie O. Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. Int Immunol. 1999;11(1):81. [PubMed]
45. Qin S, Rottman J, Myers P, Kassam N, Weinblatt M, Loetscher M, Koch A, Moser B, Mackay C. The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. Journal of Clinical Investigation. 1998;101(4):746. [PMC free article] [PubMed]
46. Bonecchi R, Bianchi G, Bordignon PP, D’Ambrosio D, Lang R, Borsatti A, Sozzani S, Allavena P, Gray PA, Mantovani A, Sinigaglia F. Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s. J Exp Med. 1998;187(1):129. [PMC free article] [PubMed]
47. Labonte ML, Letvin NL. Variable NKG2 expression in the peripheral blood lymphocytes of rhesus monkeys. Clin Exp Immunol. 2004;138(2):205. [PMC free article] [PubMed]
48. Marusina AI, Kim DK, Lieto LD, Borrego F, Coligan JE. GATA-3 is an important transcription factor for regulating human NKG2A gene expression. J Immunol. 2005;174(4):2152. [PubMed]
49. Zheng W, Flavell RA. The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells. Cell. 1997;89:587–596. [PubMed]
50. Takemoto N, Koyano-Nakagawa N, Yokota T, Arai N, Miyatake S, Arai K. Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region. Int Immunol. 1998;10(12):1981. [PubMed]
51. Agarwal S, Rao A. Modulation of chromatin structure regulates cytokine gene expression during T cell differentiation. Immunity. 1998;9:765–775. [PubMed]
52. Lee GR, Fields PE, Flavell RA. Regulation of IL-4 gene expression by distal regulatory elements and GATA-3 at the chromatin level. Immunity. 2001;14:447–459. [PubMed]
53. Loots GG, Locksley RM, Blankespoor CM, Wang ZE, Miller W, Rubin EM, Frazer KA. Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons. Science. 2000;288(5463):136. [PubMed]
54. Ranganath S, Ouyang W, Bhattarcharya D, Sha WC, Grupe A, Peltz G, Murphy KM. GATA-3-dependent enhancer activity in IL-4 gene regulation. J Immunol. 1998;161(8):3822. [PubMed]
55. Takemoto N, Kamogawa Y, Jun Lee H, Kurata H, Arai KI, O’Garra A, Arai N, Miyatake S. Cutting edge: chromatin remodeling at the IL-4/IL-13 intergenic regulatory region for Th2-specific cytokine gene cluster. J Immunol. 2000;165(12):6687. [PubMed]
56. Mohrs M, Blankespoor CM, Wang ZE, Loots GG, Afzal V, Hadeiba H, Shinkai K, Rubin EM, Locksley RM. Deletion of a coordinate regulator of type 2 cytokine expression in mice. Nat Immunol. 2001;2(9):842. [PubMed]
57. Ouyang W, et al. Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment. Immunity. 2000;12:27–37. [PubMed]
58. Ranganath S, Murphy KM. Structure and specificity of GATA proteins in Th2 development. Molecular Cellular Biology. 2001;21:2716–2725. [PMC free article] [PubMed]
59. Lopez-Botet M, Llano M, Ortega M. Human cytomegalovirus and natural killer-mediated surveillance of HLA class I expression: a paradigm of host-pathogen adaptation. Immunol Rev. 2001;181:193. [PubMed]
60. Wu J, Chalupny NJ, Manley TJ, Riddell SR, Cosman D, Spies T. Intracellular retention of the MHC class I-related chain B ligand of NKG2D by the human cytomegalovirus UL16 glycoprotein. J Immunol. 2003;170(8):4196. [PubMed]
61. Kawamura H, Yagita H, Nisizawa T, Izumi N, Miyaji C, Vance RE, Raulet DH, Okumura K, Abo T. Amelioration of acute graft-versus-host disease by NKG2A engagement on donor T cells. Eur J Immunol. 2005;35(8):2358. [PubMed]
PubReader format: click here to try


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...