Figure 7.
Expression of podoplanin and endothelial markers (CD31, CD34 and factor VIII related antigen) exemplified in a dermal high grade (G3) epitheloid angiosarcoma (Patient A. K., Table 2 ![[triangle]](/corehtml/pmc/pmcents/rtrif.gif)
). Consecutive paraffin sections were stained with hematoxylin and eosin (A) and immunolabeled for localization of podoplanin (B and C), CD34 (D), factor VIII-related antigen (E), and CD31 (F). In approximately 30% of tumor cells podoplanin was detected. Most podoplanin-expressing cells formed small groups and clusters that were frequently associated with vascular clefts (arrowheads). Individual tumor cells were also found outside these groups (B, inset). Occasionally vasoformative structures contain both podoplanin-positive and -negative lining cells (C, asterisk). Small vascular clefts without podoplanin-containing cells are prominent in some areas of the tumor (C, arrows). Although the distribution of CD34 was similar to podoplanin (D), factor VIII-related antigen (E) and CD31 (F) are more widely distributed in this tumor. Magnification, ×450.
). Consecutive paraffin sections were stained with hematoxylin and eosin (A) and immunolabeled for localization of podoplanin (B and C), CD34 (D), factor VIII-related antigen (E), and CD31 (F). In approximately 30% of tumor cells podoplanin was detected. Most podoplanin-expressing cells formed small groups and clusters that were frequently associated with vascular clefts (arrowheads). Individual tumor cells were also found outside these groups (B, inset). Occasionally vasoformative structures contain both podoplanin-positive and -negative lining cells (C, asterisk). Small vascular clefts without podoplanin-containing cells are prominent in some areas of the tumor (C, arrows). Although the distribution of CD34 was similar to podoplanin (D), factor VIII-related antigen (E) and CD31 (F) are more widely distributed in this tumor. Magnification, ×450.