• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Nov 1990; 56(11): 3389–3394.
PMCID: PMC184958

Effect of Oxygenation on Xylose Fermentation by Pichia stipitis

Abstract

The effect of oxygen limitation on xylose fermentation by Pichia stipitis (CBS 6054) was investigated in continuous culture. The maximum specific ethanol productivity (0.20 g of ethanol g dry weight−1 h−1) and ethanol yield (0.48 g/g) was reached at an oxygen transfer rate below 1 mmol/liter per h. In the studied range of oxygenation, the xylose reductase (EC 1.1.1.21) and xylitol dehydrogenase (EC 1.1.1.9) activities were constant as well as the ratio between the NADPH and NADH activities of xylose reductase. No xylitol production was found. The pyruvate decarboxylase (EC 4.1.1.1) activity increased and the malate dehydrogenase (EC 1.1.1.37) activity decreased with decreasing oxygenation. With decreasing oxygenation, the intracellular intermediary metabolites sedoheptulose 7-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate, and malate accumulated slightly while pyruvate decreased. The ratio of the xylose uptake rate under aerobic conditions, in contrast to that under anaerobic assay conditions, increased with increasing oxygenation in the culture. The results are discussed in relation to the energy level in the cell, the redox balance, and the mitochondrial function.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (997K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Does AL, Bisson LF. Characterization of Xylose Uptake in the Yeasts Pichia heedii and Pichia stipitis. Appl Environ Microbiol. 1989 Jan;55(1):159–164. [PMC free article] [PubMed]
  • Lohmeier-Vogel E, Skoog K, Vogel H, Hahn-Hägerdal B. 31P nuclear magnetic resonance study of the effect of azide on xylose fermentation by Candida tropicalis. Appl Environ Microbiol. 1989 Aug;55(8):1974–1980. [PMC free article] [PubMed]
  • Toivola A, Yarrow D, van den Bosch E, van Dijken JP, Scheffers WA. Alcoholic Fermentation of d-Xylose by Yeasts. Appl Environ Microbiol. 1984 Jun;47(6):1221–1223. [PMC free article] [PubMed]
  • van Urk H, Schipper D, Breedveld GJ, Mak PR, Scheffers WA, van Dijken JP. Localization and kinetics of pyruvate-metabolizing enzymes in relation to aerobic alcoholic fermentation in Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621. Biochim Biophys Acta. 1989 Jul 21;992(1):78–86. [PubMed]
  • Verduyn C, Van Kleef R, Frank J, Schreuder H, Van Dijken JP, Scheffers WA. Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis. Biochem J. 1985 Mar 15;226(3):669–677. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...