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Appl Environ Microbiol. Oct 1990; 56(10): 2994–2998.
PMCID: PMC184889

Extracellular proteases from Xanthomonas campestris pv. campestris, the black rot pathogen.

Abstract

Two proteases (PRT1 and PRT2) were fractionated from culture supernatants of wild-type Xanthomonas campestris pv. campestris by cation-exchange chromatography on SP-5PW. Inhibitor experiments showed that PRT 1 was a serine protease which required calcium ions for activity or stability or both and that PRT 2 was a zinc-requiring metalloprotease. PRT 1 and PRT 2 showed different patterns of degradation of beta-casein. The two proteases comprised almost all of the extracellular proteolytic activity of the wild type. A protease-deficient mutant which lacked both PRT 1 and PRT 2 showed considerable loss of virulence in pathogenicity tests when bacteria were introduced into mature turnip leaves through cut vein endings. This suggests that PRT 1 and PRT 2 have a role in black rot pathogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Daniels MJ, Barber CE, Turner PC, Sawczyc MK, Byrde RJ, Fielding AH. Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1. EMBO J. 1984 Dec 20;3(13):3323–3328. [PMC free article] [PubMed]
  • Delepelaire P, Wandersman C. Protease secretion by Erwinia chrysanthemi. Proteases B and C are synthesized and secreted as zymogens without a signal peptide. J Biol Chem. 1989 May 25;264(15):9083–9089. [PubMed]
  • Frömmel C, Sander C. Thermitase, a thermostable subtilisin: comparison of predicted and experimental structures and the molecular cause of thermostability. Proteins. 1989;5(1):22–37. [PubMed]
  • Hahn MG, Darvill AG, Albersheim P. Host-Pathogen Interactions : XIX. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS. Plant Physiol. 1981 Nov;68(5):1161–1169. [PMC free article] [PubMed]
  • Liu YN, Tang JL, Clarke BR, Dow JM, Daniels MJ. A multipurpose broad host range cloning vector and its use to characterise an extracellular protease gene of Xanthomonas campestris pathovar campestris. Mol Gen Genet. 1990 Feb;220(3):433–440. [PubMed]
  • Spungin A, Blumberg S. Streptomyces griseus aminopeptidase is a calcium-activated zinc metalloprotein. Purification and properties of the enzyme. Eur J Biochem. 1989 Aug 1;183(2):471–477. [PubMed]
  • Wilkes SH, Prescott JM. Aeromonas neutral protease. Methods Enzymol. 1976;45:404–415. [PubMed]

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