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Appl Environ Microbiol. Jul 1990; 56(7): 2268–2270.
PMCID: PMC184598

Chromium reduction in Pseudomonas putida.


Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.

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Selected References

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