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Appl Environ Microbiol. 1990 Jul; 56(7): 2251–2254.
PMCID: PMC184593

Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27.


The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid of Thermus cryptic plasmid pTT8, and beta-galactosidases were expressed constitutively.

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Selected References

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  • Casadaban MJ, Cohen SN. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. [PubMed]
  • Eberhard MD, Vásquez C, Valenzuela P, Vicuña R, Yudelevich A. Physical characterization of a plasmid (pTT1) isolated from Thermus thermophilus. Plasmid. 1981 Jul;6(1):1–6. [PubMed]
  • Erickson RP, Steers E., Jr Comparative study of isoenzyme formation of bacterial beta-galactosidase. J Bacteriol. 1970 Apr;102(1):79–84. [PMC free article] [PubMed]
  • Hishinuma F, Tanaka T, Sakaguchi K. Isolation of extrachromosomal deoxyribonucleic acids from extremely thermophilic bacteria. J Gen Microbiol. 1978 Feb;104(2):193–199. [PubMed]
  • Russell DR, Bennett GN. Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing. Gene. 1982 Dec;20(2):231–243. [PubMed]
  • Silhavy TJ, Beckwith JR. Uses of lac fusions for the study of biological problems. Microbiol Rev. 1985 Dec;49(4):398–418. [PMC free article] [PubMed]
  • Ulrich JT, McFeters GA, Temple KL. Induction and characterization of -galactosidase in an extreme thermophile. J Bacteriol. 1972 May;110(2):691–698. [PMC free article] [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]

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