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Journal List > Appl Environ Microbiol > v.56(4); Apr 1990

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Appl Environ Microbiol. 1990 April; 56(4): 1188–1190.
PMCID: PMC184370
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Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Pseudomonas fluorescens Proteases in Ultrahigh-Temperature-Treated Milk
Richard S. Clements,1†* David M. Wyatt,1‡ Michael H. Symons,2 and Kenneth N. Ewings2
1Immunology Department, Queensland University of Technology, Brisbane, 4000, and Department of Primary Industries, Queensland Food Research Laboratories, Hamilton, 4007,2 Queensland, Australia
* Corresponding author.
† Present address: Bunge Scientific, 89 Flemington Rd., North Melbourne, 3051, Victoria, Australia.
‡ Present address: Queensland Medical Laboratories, West End, 4101, Queensland, Australia.
Abstract
An inhibition enzyme-linked immunosorbent assay was developed to detect low levels of the proteases extracted from four strains of Pseudomonas fluorescens. The assay detected between 0.24 and 7.8 ng of protease per ml of ultrahigh-temperature-treated milk and could be completed within 6 h. It could be used as a framework for a test system for quantifying spoilage proteases in dairy products.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Adams DM, Barach JT, Speck ML. Heat resistant proteases produced in milk by psychrotrophic bacteria of dairy origin. J Dairy Sci. 1975 Jun;58(6):828–834. [PubMed]
  • Barach JT, Adams DM, Speck ML. Stabilization of a psychrotrophic Pseudomonas protease by calcium against thermal inactivation in milk at ultrahigh temperature. Appl Environ Microbiol. 1976 Jun;31(6):875–879. [PubMed]
  • Birkeland SE, Stepaniak L, Sørhaug T. Quantitative studies of heat-stable proteinase from Pseudomonas fluorescens P1 by the enzyme-linked immunosorbent assay. Appl Environ Microbiol. 1985 Feb;49(2):382–387. [PubMed]
  • Law MP. Prospects for hyperthermia in cancer therapy. Radiography. 1982 Oct;48(574):209–219. [PubMed]
  • Jackman DM, Bartlett FM, Patel TR. Heat-stable proteases from psychrotrophic pseudomonads: comparison of immunological properties. Appl Environ Microbiol. 1983 Jul;46(1):6–12. [PubMed]
  • Mayerhofer HJ, Marshall RT, White CH, Lu M. Characterization of a heat-stable protease of Pseudomonas fluorescens P26. Appl Microbiol. 1973 Jan;25(1):44–48. [PubMed]
  • O'Connor RE, Ewings KN, Hayward AC, O'Rourke PK. Numerical taxonomy of proteolytic psychrotrophs from Queensland raw milks. J Appl Bacteriol. 1986 Jul;61(1):25–38. [PubMed]
  • Rylatt DB, Parish CR. Protein determination on an automatic spectrophotometer. Anal Biochem. 1982 Mar 15;121(1):213–214. [PubMed]
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