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Appl Environ Microbiol. Oct 1991; 57(10): 3046–3048.
PMCID: PMC183920

Development of an improved chemically defined minimal medium for Listeria monocytogenes.

Abstract

A chemically defined minimal medium for Listeria monocytogenes has been developed by modification of Welshimer's medium. The growth factors required by L. monocytogenes Scott A are leucine, isoleucine, arginine, methionine, valine, cysteine (each at 100 mg/liter), riboflavin and biotin (each at 0.5 micrograms/ml), thiamine (1.0 micrograms/ml), and thioctic acid (0.005 micrograms/ml). Growth was stimulated by 20 micrograms of Fe3+ per ml as ferric citrate. Glucose (1%) and glutamine (600 mg/liter) are required as primary sources of carbon and nitrogen. Glucose could not be replaced by various organic acids or amino acids. Of several sugars tested, fructose, mannose, cellobiose, trehalose, maltose (weak), glycerol (weak), and the amino sugars glucosamine, N-acetylglucosamine, and N-acetylmuramic acid supported growth in the absence of glucose. Evidence was found that chitin and cell walls of starter bacteria (Lactococcus lactis) supported survival of L. monocytogenes, which suggests that the pathogen may obtain carbon and energy sources during colonization of some foods, such as cheeses, by assimilating bacteria or molds that are present.

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Selected References

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  • Berenguer J, Faraldo ML, de Pedro MA. Ca2+-stabilized oligomeric protein complexes are major components of the cell envelope of "Thermus thermophilus" HB8. J Bacteriol. 1988 Jun;170(6):2441–2447. [PMC free article] [PubMed]
  • Cowart RE, Foster BG. Differential effects of iron on the growth of Listeria monocytogenes: minimum requirements and mechanism of acquisition. J Infect Dis. 1985 Apr;151(4):721–730. [PubMed]
  • FRIEDMAN ME, ROESSLER WG. Growth of Listeria monocytogenes in defined media. J Bacteriol. 1961 Oct;82:528–533. [PMC free article] [PubMed]
  • Gellin BG, Broome CV. Listeriosis. JAMA. 1989 Mar 3;261(9):1313–1320. [PubMed]
  • Pearson LJ, Marth EH. Listeria monocytogenes--threat to a safe food supply: a review. J Dairy Sci. 1990 Apr;73(4):912–928. [PubMed]
  • Pine L, Malcolm GB, Brooks JB, Daneshvar MI. Physiological studies on the growth and utilization of sugars by Listeria species. Can J Microbiol. 1989 Feb;35(2):245–254. [PubMed]
  • Schwartz B, Ciesielski CA, Broome CV, Gaventa S, Brown GR, Gellin BG, Hightower AW, Mascola L. Association of sporadic listeriosis with consumption of uncooked hot dogs and undercooked chicken. Lancet. 1988 Oct 1;2(8614):779–782. [PubMed]
  • Siddiqi R, Khan MA. Vitamin and nitrogen base requirements for Listeria monocytogenes and haemolysin production. Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Nov;253(2):225–235. [PubMed]
  • Siddiqi R, Khan MA. Amino acid requirement of six strains of Listeria monocytogenes. Zentralbl Bakteriol. 1989 Jul;271(2):146–152. [PubMed]
  • Sword CP. Mechanisms of pathogenesis in Listeria monocytogenes infection. I. Influence of iron. J Bacteriol. 1966 Sep;92(3):536–542. [PMC free article] [PubMed]
  • Trivett TL, Meyer EA. Citrate cycle and related metabolism of Listeria monocytogenes. J Bacteriol. 1971 Sep;107(3):770–779. [PMC free article] [PubMed]
  • WELSHIMER HJ. VITAMIN REQUIREMENTS OF LISTERIA MONOCYTOGENES. J Bacteriol. 1963 May;85:1156–1159. [PMC free article] [PubMed]

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