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Appl Environ Microbiol. Jun 1991; 57(6): 1683–1688.
PMCID: PMC183452

Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat.

Abstract

Ten strains of bacteriocin-producing lactic acid bacteria were isolated from retail cuts of meat. These 10 strains along with 11 other bacteriocin-producing lactic acid bacteria were tested for inhibitory activity against psychotrophic pathogens, including four strains of Listeria monocytogenes, two strains of Aeromonas hydrophila, and two strains of Staphylococcus aureus. Inhibition due to acid, hydrogen peroxide, and lytic bacteriophage were excluded. The proteinaceous nature of the inhibitory substance was confirmed by demonstration of its sensitivity to proteolytic enzymes. Eight of the meat isolates had inhibitory activity against all four L. monocytogenes strains. Bacteriocin activity against L. monocytogenes was found in all of the strains obtained from other sources. Activity against A. hydrophila and S. aureus was also common.

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Selected References

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  • Ahn C, Stiles ME. Antibacterial activity of lactic acid bacteria isolated from vacuum-packaged meats. J Appl Bacteriol. 1990 Sep;69(3):302–310. [PubMed]
  • Anderson DG, McKay LL. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. [PMC free article] [PubMed]
  • Barefoot SF, Klaenhammer TR. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol. 1983 Jun;45(6):1808–1815. [PMC free article] [PubMed]
  • Barefoot SF, Klaenhammer TR. Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob Agents Chemother. 1984 Sep;26(3):328–334. [PMC free article] [PubMed]
  • Bhunia AK, Johnson MC, Ray B. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol. 1988 Oct;65(4):261–268. [PubMed]
  • Fleming DW, Cochi SL, MacDonald KL, Brondum J, Hayes PS, Plikaytis BD, Holmes MB, Audurier A, Broome CV, Reingold AL. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. [PubMed]
  • Gilbert RJ, Miller KL, Roberts D. Listeria monocytogenes and chilled foods. Lancet. 1989 Feb 18;1(8634):383–384. [PubMed]
  • Golden DA, Eyles MJ, Beuchat LR. Influence of modified-atmosphere storage on the growth of uninjured and heat-injured Aeromonas hydrophila. Appl Environ Microbiol. 1989 Nov;55(11):3012–3015. [PMC free article] [PubMed]
  • Joerger MC, Klaenhammer TR. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol. 1986 Aug;167(2):439–446. [PMC free article] [PubMed]
  • Kékessy DA, Piguet JD. New method for detecting bacteriocin production. Appl Microbiol. 1970 Aug;20(2):282–283. [PMC free article] [PubMed]
  • Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie. 1988 Mar;70(3):337–349. [PubMed]
  • Liu W, Hansen JN. Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis. Appl Environ Microbiol. 1990 Aug;56(8):2551–2558. [PMC free article] [PubMed]
  • Macrina FL, Kopecko DJ, Jones KR, Ayers DJ, McCowen SM. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. [PubMed]
  • Muriana PM, Klaenhammer TR. Conjugal Transfer of Plasmid-Encoded Determinants for Bacteriocin Production and Immunity in Lactobacillus acidophilus 88. Appl Environ Microbiol. 1987 Mar;53(3):553–560. [PMC free article] [PubMed]
  • Muriana PM, Klaenhammer TR. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl Environ Microbiol. 1991 Jan;57(1):114–121. [PMC free article] [PubMed]
  • Pátek M, Hochmannová J, Nesvera J, Stránský J. Glutamicin CBII, a bacteriocin-like substance produced by Corynebacterium glutamicum. Antonie Van Leeuwenhoek. 1986;52(2):129–140. [PubMed]
  • Schillinger U, Lücke FK. Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol. 1989 Aug;55(8):1901–1906. [PMC free article] [PubMed]
  • Tagg JR, Dajani AS, Wannamaker LW. Bacteriocins of gram-positive bacteria. Bacteriol Rev. 1976 Sep;40(3):722–756. [PMC free article] [PubMed]
  • van Belkum MJ, Hayema BJ, Geis A, Kok J, Venema G. Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid. Appl Environ Microbiol. 1989 May;55(5):1187–1191. [PMC free article] [PubMed]

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