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Appl Environ Microbiol. 1992 Sep; 58(9): 2886–2893.
PMCID: PMC183023

Metal regulation of siderophore synthesis in Pseudomonas aeruginosa and functional effects of siderophore-metal complexes.


Pseudomonas aeruginosa synthesizes two siderophores, pyochelin and pyoverdin, characterized by widely different structures, physicochemical properties, and affinities for Fe(III). Titration experiments showed that pyochelin, which is endowed with a relatively low affinity for Fe(III), binds other transition metals, such as Cu(II), Co(II), Mo(VI), and Ni(II), with appreciable affinity. In line with these observations, Fe(III) and Co(II) at 10 microM or Mo(VI), Ni(II), and Cu(II) at 100 microM repressed pyochelin synthesis and reduced expression of iron-regulated outer membrane proteins of 75, 68, and 14 kDa. In contrast, pyoverdin synthesis and expression of the 80-kDa receptor protein were affected only by Fe(III). All of the metals tested, except Mo(VI), significantly promoted P. aeruginosa growth in metal-poor medium; Mo(VI), Ni(II), and Co(II) were more efficient as pyochelin complexes than the free metal ions and the siderophore. The observed correlation between the affinity of pyochelin for Fe(III), Co(II), and Mo(VI) and the functional effects of these metals indicates that pyochelin may play a role in their delivery to P. aeruginosa.

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Selected References

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  • Ankenbauer RG, Cox CD. Isolation and characterization of Pseudomonas aeruginosa mutants requiring salicylic acid for pyochelin biosynthesis. J Bacteriol. 1988 Nov;170(11):5364–5367. [PMC free article] [PubMed]
  • Ankenbauer RG, Staley AL, Rinehart KL, Cox CD. Mutasynthesis of siderophore analogues by Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1878–1882. [PMC free article] [PubMed]
  • Briskot G, Taraz K, Budzikiewicz H. Siderophore vom Pyoverdin-Typ aus Pseudomonas aeruginosa. Z Naturforsch C. 1986 May-Jun;41(5-6):497–506. [PubMed]
  • Cox CD, Adams P. Siderophore activity of pyoverdin for Pseudomonas aeruginosa. Infect Immun. 1985 Apr;48(1):130–138. [PMC free article] [PubMed]
  • Cox CD, Graham R. Isolation of an iron-binding compound from Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):357–364. [PMC free article] [PubMed]
  • Galan P, Hercberg S, Touitou Y. The activity of tissue enzymes in iron-deficient rat and man: an overview. Comp Biochem Physiol B. 1984;77(4):647–653. [PubMed]
  • Guterman SK. Colicin B: mode of action and inhibition by enterochelin. J Bacteriol. 1973 Jun;114(3):1217–1224. [PMC free article] [PubMed]
  • Heinrichs DE, Young L, Poole K. Pyochelin-mediated iron transport in Pseudomonas aeruginosa: involvement of a high-molecular-mass outer membrane protein. Infect Immun. 1991 Oct;59(10):3680–3684. [PMC free article] [PubMed]
  • Kustin K, Liu ST. Kinetics and complex formation of molybdate with catechol. J Am Chem Soc. 1973 Apr 18;95(8):2487–2491. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Meyer JM, Hohnadel D, Khan A, Cornelis P. Pyoverdin-facilitated iron uptake in Pseudomonas aeruginosa: immunological characterization of the ferripyoverdin receptor. Mol Microbiol. 1990 Aug;4(8):1401–1405. [PubMed]
  • Neilands JB. Microbial envelope proteins related to iron. Annu Rev Microbiol. 1982;36:285–309. [PubMed]
  • Poole K, Neshat S, Heinrichs D. Pyoverdine-mediated iron transport in Pseudomonas aeruginosa: involvement of a high-molecular-mass outer membrane protein. FEMS Microbiol Lett. 1991 Feb;62(1):1–5. [PubMed]
  • Sokol PA, Woods DE. Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa. Infect Immun. 1983 May;40(2):665–669. [PMC free article] [PubMed]
  • Weinberg ED. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. [PMC free article] [PubMed]
  • Williams PH. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. [PMC free article] [PubMed]

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