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Appl Environ Microbiol. May 1991; 57(5): 1546–1553.
PMCID: PMC182983

Transfer and Expression of the Catabolic Plasmid pBRC60 in Wild Bacterial Recipients in a Freshwater Ecosystem


3-Chlorobenzoate (3Cba)-degrading bacteria were isolated from the waters and sediments of flowthrough mesocosms dosed with various concentrations of 3Cba and inoculated with a 3Cba-degrading Alcaligenes sp., strain BR60. Bacteria capable of 3Cba degradation which were distinct from BR60 were isolated. They carried pBRC60, a plasmid introduced with Alcaligenes sp. strain BR60 that carries a transposable element (Tn5271) encoding 3Cba degradation. The isolates expressed these genes in different ways. The majority of pBRC60 recipients were motile, yellow-pigmented, gram-negative rods related to the group III pseudomonads and to BR60 by substrate utilization pattern. They were capable of complete 3Cba degradation at both millimolar and micromolar concentrations. Two isolates, Pseudomonas fluorescens PR24B(pBRC60) and Pseudomonas sp. strain PR120(pBRC60), are more distantly related to BR60 and both produced chlorocatechol when exposed to 3Cba at millimolar concentrations in the presence of yeast extract. These species showed poor growth in liquid 3Cba minimal medium but could degrade 3Cba in continuous cultures dosed with micromolar levels of the chemical. Laboratory matings confirm that pBRC60 can transfer from BR60 to species in both the beta and gamma subgroups of the proteobacteria and that 3Cba gene expression is variable between species. Selection pressures acting on pBRC60 recipients are discussed.

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  • Bale MJ, Fry JC, Day MJ. Plasmid transfer between strains of Pseudomonas aeruginosa on membrane filters attached to river stones. J Gen Microbiol. 1987 Nov;133(11):3099–3107. [PubMed]
  • Caldwell BA, Ye C, Griffiths RP, Moyer CL, Morita RY. Plasmid expression and maintenance during long-term starvation-survival of bacteria in well water. Appl Environ Microbiol. 1989 Aug;55(8):1860–1864. [PMC free article] [PubMed]
  • Carhart G, Hegeman G. Improved method of selection for mutants of Pseudomonas putida. Appl Microbiol. 1975 Dec;30(6):1046–1047. [PMC free article] [PubMed]
  • Caulcott CA, Dunn A, Robertson HA, Cooper NS, Brown ME, Rhodes PM. Investigation of the effect of growth environment on the stability of low-copy-number plasmids in Escherichia coli. J Gen Microbiol. 1987 Jul;133(7):1881–1889. [PubMed]
  • Chatfield LK, Williams PA. Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes. J Bacteriol. 1986 Nov;168(2):878–885. [PMC free article] [PubMed]
  • Chatterjee DK, Chakrabarty AM. Genetic homology between independently isolated chlorobenzoate-degradative plasmids. J Bacteriol. 1983 Jan;153(1):532–534. [PMC free article] [PubMed]
  • Cruz-Cruz NE, Toranzos GA, Ahearn DG, Hazen TC. In situ survival of plasmid-bearing and plasmidless Pseudomonas aeruginosa in pristine tropical waters. Appl Environ Microbiol. 1988 Oct;54(10):2574–2577. [PMC free article] [PubMed]
  • de Taxis du Poët P, Arcand Y, Bernier R, Jr, Barbotin JN, Thomas D. Plasmid stability in immobilized and free recombinant Escherichia coli JM105(pKK223-200): importance of oxygen diffusion, growth rate, and plasmid copy number. Appl Environ Microbiol. 1987 Jul;53(7):1548–1555. [PMC free article] [PubMed]
  • Fulthorpe RR, Wyndham RC. Survival and activity of a 3-chlorobenzoate-catabolic genotype in a natural system. Appl Environ Microbiol. 1989 Jun;55(6):1584–1590. [PMC free article] [PubMed]
  • Gealt MA, Chai MD, Alpert KB, Boyer JC. Transfer of plasmids pBR322 and pBR325 in wastewater from laboratory strains of Escherichia coli to bacteria indigenous to the waste disposal system. Appl Environ Microbiol. 1985 Apr;49(4):836–841. [PMC free article] [PubMed]
  • Genthner FJ, Chatterjee P, Barkay T, Bourquin AW. Capacity of aquatic bacteria to act as recipients of plasmid DNA. Appl Environ Microbiol. 1988 Jan;54(1):115–117. [PMC free article] [PubMed]
  • Ghosal D, You IS, Chatterjee DK, Chakrabarty AM. Genes specifying degradation of 3-chlorobenzoic acid in plasmids pAC27 and pJP4. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1638–1642. [PMC free article] [PubMed]
  • Henis Y, Alexander M. Effect of organic amendments on bacterial multiplication in lake water. Antonie Van Leeuwenhoek. 1990 Jan;57(1):9–20. [PubMed]
  • KING EO, WARD MK, RANEY DE. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed]
  • Klein TM, Alexander M. Bacterial inhibitors in lake water. Appl Environ Microbiol. 1986 Jul;52(1):114–118. [PMC free article] [PubMed]
  • O'Morchoe SB, Ogunseitan O, Sayler GS, Miller RV. Conjugal transfer of R68.45 and FP5 between Pseudomonas aeruginosa strains in a freshwater environment. Appl Environ Microbiol. 1988 Aug;54(8):1923–1929. [PMC free article] [PubMed]
  • Pertsova RN, Kunc F, Golovleva LA. Degradation of 3-chlorobenzoate in soil by pseudomonads carrying biodegradative plasmids. Folia Microbiol (Praha) 1984;29(3):242–247. [PubMed]
  • Pickett MJ, Pedersen MM. Nonfermentative bacilli associated with man. II. Detection and identification. Am J Clin Pathol. 1970 Aug;54(2):164–177. [PubMed]
  • Roszak DB, Colwell RR. Survival strategies of bacteria in the natural environment. Microbiol Rev. 1987 Sep;51(3):365–379. [PMC free article] [PubMed]
  • Scheuerman PR, Schmidt JP, Alexander M. Factors affecting the survival and growth of bacteria introduced into lake water. Arch Microbiol. 1988;150(4):320–325. [PubMed]
  • Selvaraj G, Iyer VN. Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria. J Bacteriol. 1983 Dec;156(3):1292–1300. [PMC free article] [PubMed]
  • Stotzky G, Babich H. Survival of, and genetic transfer by, genetically engineered bacteria in natural environments. Adv Appl Microbiol. 1986;31:93–138. [PubMed]
  • Warnes A, Stephenson JR. The insertion of large pieces of foreign genetic material reduces the stability of bacterial plasmids. Plasmid. 1986 Sep;16(2):116–123. [PubMed]
  • Lee SW, Edlin G. Expression of tetracycline resistance in pBR322 derivatives reduces the reproductive fitness of plasmid-containing Escherichia coli. Gene. 1985;39(2-3):173–180. [PubMed]
  • Wyndham RC. Evolved aniline catabolism in Acinetobacter calcoaceticus during continuous culture of river water. Appl Environ Microbiol. 1986 Apr;51(4):781–789. [PMC free article] [PubMed]
  • Wyndham RC, Singh RK, Straus NA. Catabolic instability, plasmid gene deletion and recombination in Alcaligenes sp. BR60. Arch Microbiol. 1988;150(3):237–243. [PubMed]
  • Wyndham RC, Straus NA. Chlorobenzoate catabolism and interactions between Alcaligenes and Pseudomonas species from Bloody Run Creek. Arch Microbiol. 1988;150(3):230–236. [PubMed]
  • Zeyer J, Wasserfallen A, Timmis KN. Microbial mineralization of ring-substituted anilines through an ortho-cleavage pathway. Appl Environ Microbiol. 1985 Aug;50(2):447–453. [PMC free article] [PubMed]

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