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Appl Environ Microbiol. Nov 1993; 59(11): 3666–3668.
PMCID: PMC182514

Production of Rhizobium Inoculants for Lupinus nootkatensis on Nutrient-Supplemented Pumice


The use of the legume Lupinus nootkatensis as a pioneer plant to fight soil erosion and to reclaim eroded soils in Iceland has been under development for a few years. Production of a robust, low-cost bacterial inoculant was therefore a prerequisite for the extended use of this plant. Volcanic pumice is a naturally expanded mineral which is available in vast amounts in Iceland. It was tested as a carrier for solid fermentation of Rhizobium lupini. Nutrient-supplemented pumice containing a small percentage of peat and diatomaceous earth and kept in sterile plastic bags promoted good growth of the bacteria. Viable-colony counts remained stable at 108 to 109/g for at least 35 weeks when the carrier was stored at 22°C. The pumice-based inoculant had good storage and handling properties and could be mixed directly with the seeds during the sowing process. When seeds of L. nootkatensis were sown manually into nutrient-poor eroded sandy soils, about 56% of the first-year plants were successfully nodulated.

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Selected References

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  • Graham-Weiss L, Bennett ML, Paau AS. Production of bacterial inoculants by direct fermentation on nutrient-supplemented vermiculite. Appl Environ Microbiol. 1987 Sep;53(9):2138–2141. [PMC free article] [PubMed]
  • Strijdom BW, van Rensburg HJ. Effect of steam sterilization and gamma irradiation of peat on quality of Rhizobium inoculants. Appl Environ Microbiol. 1981 Jun;41(6):1344–1347. [PMC free article] [PubMed]

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