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Appl Environ Microbiol. 1993 November; 59(11): 3666–3668.
PMCID: PMC182514
Production of Rhizobium Inoculants for Lupinus nootkatensis on Nutrient-Supplemented Pumice
Sigurbjorn Einarsson,1* Jon Gudmundsson,2 Halldor Sverrisson,2 Jakob K. Kristjansson,1,3 and Sveinn Runolfsson4
1Department of Biotechnology, Technological Institute of Iceland, and Agricultural Research Station, 2 Keldnaholt, IS-112 Reykjavík, Institute of Biology, University of Iceland, IS-108 Reykjavík, 3 and Soil Conservation Service of Iceland, Gunnarsholt, IS-850 Hella, 4 Iceland
* Corresponding author.
Abstract
The use of the legume Lupinus nootkatensis as a pioneer plant to fight soil erosion and to reclaim eroded soils in Iceland has been under development for a few years. Production of a robust, low-cost bacterial inoculant was therefore a prerequisite for the extended use of this plant. Volcanic pumice is a naturally expanded mineral which is available in vast amounts in Iceland. It was tested as a carrier for solid fermentation of Rhizobium lupini. Nutrient-supplemented pumice containing a small percentage of peat and diatomaceous earth and kept in sterile plastic bags promoted good growth of the bacteria. Viable-colony counts remained stable at 108 to 109/g for at least 35 weeks when the carrier was stored at 22°C. The pumice-based inoculant had good storage and handling properties and could be mixed directly with the seeds during the sowing process. When seeds of L. nootkatensis were sown manually into nutrient-poor eroded sandy soils, about 56% of the first-year plants were successfully nodulated.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Graham-Weiss Lori, Bennett Mari Lynn, Paau Alan S. Production of Bacterial Inoculants by Direct Fermentation on Nutrient-Supplemented Vermiculite. Appl Environ Microbiol. 1987 Sep;53(9):2138–2141. [PubMed]
  • Strijdom Barend W, van Rensburg Henri Jansen. Effect of Steam Sterilization and Gamma Irradiation of Peat on Quality of Rhizobium Inoculants. Appl Environ Microbiol. 1981 Jun;41(6):1344–1347. [PubMed]