• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Oct 1993; 59(10): 3360–3366.
PMCID: PMC182459

Variations in the uptake and metabolism of peptides and amino acids by mixed ruminal bacteria in vitro.

Abstract

Mixed ruminal bacteria, isolated from sheep (Q and W) fed a concentrate and hay diet, were anaerobically incubated with either 14C-peptides or 14C-amino acids. Experiment 1 showed that uptake of both 14C-labeled substrates was rapid, but the rate for amino acids was twofold greater than for peptides (molecular weight, 1,000 to 200) initially but was similar after 10 min. Experiment 2 demonstrated that metabolism was also rapid; at least 90% of either 14C-labeled substrate was metabolized by 3 min. Of the radioactivity remaining in bacteria, approximately 30% was in the form of 14C-amino acids, but only in leucine, tyrosine, and phenylalanine. Supernatant radioactivity was contained only in tyrosine, phenylalanine, and mostly proline for incubations with 14C-amino acids but in up to 10 amino acids when 14C-peptides were the substrates. Short-term incubations (< 5 min; experiment 3) confirmed previous uptake patterns and showed that the experimental system was responsive to substrate competition. Experiment 4 demonstrated that bacteria from sheep Q possessed initial and maximum rates of 14C-amino acid uptake approximately fourfold greater (P < 0.01) than those of 14C-peptides, but with no significant differences (P > 0.1) between four 14C-peptide substrate groups with molecular weights of 2,000 to < 200. By contrast, bacteria from sheep W showed no such distinctions (P > 0.1) between rates for 14C-peptides and 14C-amino acids. Calculations suggested that peptides could supply from 11 to 35% and amino acids could supply from 36 to 68% of the N requirements of mixed ruminal bacteria.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Argyle JL, Baldwin RL. Effects of amino acids and peptides on rumen microbial growth yields. J Dairy Sci. 1989 Aug;72(8):2017–2027. [PubMed]
  • Armstead IP, Ling JR. Chromatographic separation of mixed peptides from amino acids in biological digests with volatile buffers. J Chromatogr. 1991 Nov 22;586(2):259–263. [PubMed]
  • Bladen HA, Bryant MP, Doetsch RN. A Study of Bacterial Species from the Rumen Which Produce Ammonia from Protein Hydrolyzate. Appl Microbiol. 1961 Mar;9(2):175–180. [PMC free article] [PubMed]
  • Chalupa W. Degradation of amino acids by the mixed rumen microbial population. J Anim Sci. 1976 Oct;43(4):828–834. [PubMed]
  • Chen GJ, Russell JB. Fermentation of peptides and amino acids by a monensin-sensitive ruminal Peptostreptococcus. Appl Environ Microbiol. 1988 Nov;54(11):2742–2749. [PMC free article] [PubMed]
  • Chen G, Russell JB. More monensin-sensitive, ammonia-producing bacteria from the rumen. Appl Environ Microbiol. 1989 May;55(5):1052–1057. [PMC free article] [PubMed]
  • Chen G, Russell JB, Sniffen CJ. A procedure for measuring peptides in rumen fluid and evidence that peptide uptake can be a rate-limiting step in ruminal protein degradation. J Dairy Sci. 1987 Jun;70(6):1211–1219. [PubMed]
  • Chen G, Sniffen CJ, Russell JB. Concentration and estimated flow of peptides from the rumen of dairy cattle: effects of protein quantity, protein solubility, and feeding frequency. J Dairy Sci. 1987 May;70(5):983–992. [PubMed]
  • Chen G, Strobel HJ, Russell JB, Sniffen CJ. Effect of hydrophobicity of utilization of peptides by ruminal bacteria in vitro. Appl Environ Microbiol. 1987 Sep;53(9):2021–2025. [PMC free article] [PubMed]
  • Ling JR, Buttery PJ. The simultaneous use of ribonucleic acid, 35S, 2,6-diaminopimelic acid and 2-aminoethylphosphonic acid as markers of microbial nitrogen entering the duodenum of sheep. Br J Nutr. 1978 Jan;39(1):165–179. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Nolan JV, Norton BW, Leng RA. Further studies of the dynamics of nitrogen metabolism in sheep. Br J Nutr. 1976 Jan;35(1):127–147. [PubMed]
  • Nugent JH, Mangan JL. Characteristics of the rumen proteolysis of fraction I (18S) leaf protein from lucerne (Medicago sativa L). Br J Nutr. 1981 Jul;46(1):39–58. [PubMed]
  • PITTMAN KA, BRYANT MP. PEPTIDES AND OTHER NITROGEN SOURCES FOR GROWTH OF BACTEROIDES RUMINICOLA. J Bacteriol. 1964 Aug;88:401–410. [PMC free article] [PubMed]
  • Pittman KA, Lakshmanan S, Bryant MP. Oligopeptide uptake by Bacteroides ruminicola. J Bacteriol. 1967 May;93(5):1499–1508. [PMC free article] [PubMed]
  • Prins RA, van Hal-Van Gestel JC, Counotte GH. Degradation of amino acids and peptides by mixed rumen micro-organisms. Z Tierphysiol Tierernahr Futtermittelkd. 1979 Dec;42(6):333–339. [PubMed]
  • Russell JB, O'Connor JD, Fox DG, Van Soest PJ, Sniffen CJ. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. J Anim Sci. 1992 Nov;70(11):3551–3561. [PubMed]
  • Scheifinger C, Russell N, Chalupa W. Degradation of amino acids by pure cultures of rumen bacteria. J Anim Sci. 1976 Oct;43(4):821–827. [PubMed]
  • Stevenson RM. Amino acid uptake systems in Bacteroides ruminicola. Can J Microbiol. 1979 Oct;25(10):1161–1168. [PubMed]
  • Wallace RJ. Acetylation of peptides inhibits their degradation by rumen micro-organisms. Br J Nutr. 1992 Sep;68(2):365–372. [PubMed]
  • Wallace RJ, McKain N. Analysis of peptide metabolism by ruminal microorganisms. Appl Environ Microbiol. 1989 Sep;55(9):2372–2376. [PMC free article] [PubMed]
  • Wallace RJ, McKain N. A survey of peptidase activity in rumen bacteria. J Gen Microbiol. 1991 Sep;137(9):2259–2264. [PubMed]
  • Webb KE, Jr, Dirienzo DB, Matthews JC. Recent developments in gastrointestinal absorption and tissue utilization of peptides: a review. J Dairy Sci. 1993 Jan;76(1):351–361. [PubMed]
  • Westlake K, Mackie RI. Peptide and amino acid transport in Streptococcus bovis. Appl Microbiol Biotechnol. 1990 Oct;34(1):97–102. [PubMed]
  • Wright DE. Metabolism of peptides by rumen microorganisms. Appl Microbiol. 1967 May;15(3):547–550. [PMC free article] [PubMed]
  • Yang CM, Russell JB. Resistance of proline-containing peptides to ruminal degradation in vitro. Appl Environ Microbiol. 1992 Dec;58(12):3954–3958. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...