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Appl Environ Microbiol. Jun 1993; 59(6): 1893–1897.
PMCID: PMC182177

Effect of Initial Oxygen Concentration on Diacetyl and Acetoin Production by Lactococcus lactis subsp. lactis biovar diacetylactis

Abstract

The production of aroma compounds (acetoin and diacetyl) in fresh unripened cheese by Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 was studied at 30°C at different initial oxygen concentrations (0, 21, 50, and 100% of the medium saturation by oxygen). Regardless of the initial O2 concentration, maximal production of these compounds was reached only after all the citrate was consumed. Diacetyl and acetoin production was 0.01 and 2.4 mM, respectively, at 0% oxygen. Maximum acetoin concentration reached 5.4 mM at 100% oxygen. Diacetyl production was increased by factors of 2, 6, and 18 at initial oxygen concentrations of 21, 50, and 100%, respectively. The diacetyl/acetoin concentration ratio increased linearly with initial oxygen concentration: it was eight times higher at 100% (3.3%) than at 0% oxygen (0.4%). The effect of oxygen on diacetyl and acetoin production was also shown with other lactococci. At 0% oxygen, specific activity of α-acetolactate synthetase (0.15 U/mg) and NADH oxidase (0.04 U/mg) was 3.6 and 5.4 times lower, respectively, than at 100% oxygen. The increasing α-acetolactate synthetase activity in the presence of oxygen would explain the higher production of diacetyl and acetoin. The NADH oxidase activity would replace the role of the lactate dehydrogenase, diacetyl reductase, and acetoin reductase in the reoxidation of NADH, allowing accumulation of these two aroma compounds.

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Selected References

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  • Anders RF, Hogg DM, Jago GR. Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism. Appl Microbiol. 1970 Apr;19(4):608–612. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Bruhn JC, Collins EB. Reduced nicotinamide adenine dinucleotide oxidase of Streptococcus diacetilactis. J Dairy Sci. 1970 Jul;53(7):857–860. [PubMed]
  • Drinan DF, Robin S, Cogan TM. Citric acid metabolism in hetero- and homofermentative lactic acid bacteria. Appl Environ Microbiol. 1976 Apr;31(4):481–486. [PMC free article] [PubMed]
  • HARVEY RJ, COLLINS EB. Citrate transport system of Streptococcus diacetilactis. J Bacteriol. 1962 May;83:1005–1009. [PMC free article] [PubMed]
  • HARVEY RJ, COLLINS EB. ROLES OF CITRATE AND ACETOIN IN THE METABOLISM OF STREPTOCOCCUS DIACETILACTIS. J Bacteriol. 1963 Dec;86:1301–1307. [PMC free article] [PubMed]
  • Kaneko T, Takahashi M, Suzuki H. Acetoin Fermentation by Citrate-Positive Lactococcus lactis subsp. lactis 3022 Grown Aerobically in the Presence of Hemin or Cu. Appl Environ Microbiol. 1990 Sep;56(9):2644–2649. [PMC free article] [PubMed]
  • Kempler GM, McKay LL. Improved Medium for Detection of Citrate-Fermenting Streptococcus lactis subsp. diacetylactis. Appl Environ Microbiol. 1980 Apr;39(4):926–927. [PMC free article] [PubMed]
  • Kümmel A, Behrens G, Gottschalk G. Citrate lyase from Streptococcus diacetilactis. Association with its acetylating enzyme. Arch Microbiol. 1975;102(2):111–116. [PubMed]
  • Thomas TD, Turner KW, Crow VL. Galactose fermentation by Streptococcus lactis and Streptococcus cremoris: pathways, products, and regulation. J Bacteriol. 1980 Nov;144(2):672–682. [PMC free article] [PubMed]
  • Verhue WM, Tjan FS. Study of the Citrate Metabolism of Lactococcus lactis subsp. lactis Biovar Diacetylactis by Means of C Nuclear Magnetic Resonance. Appl Environ Microbiol. 1991 Nov;57(11):3371–3377. [PMC free article] [PubMed]

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