Logo of aacPermissionsJournals.ASM.orgJournalAAC ArticleJournal InfoAuthorsReviewers
Antimicrob Agents Chemother. 1981 Jan; 19(1): 139–143.
PMCID: PMC181372

Sensitive radioimmunoassay for vancomycin.


A radioimmunoassay for vancomycin has been developed which uses rabbit antiserum induced by vancomycin-bovine serum albumin conjugates and vancomycin labeled with 3H or 125I. Using either isotope, the method is simple and reproducible and has a sensitivity of 4 or 0.04 ng/ml, depending on the tracer used. This is 200- to 20,000-fold improvement in sensitivity compared with the most sensitive bioassay. Drug levels in serum or urine samples from patients receiving vancomycin can be determined by this assay procedure without processing. The data obtained with 3H and 125I labels were in good agreement. Patients' plasma vancomycin concentrations determined by radioimmunoassay correlated well with those determined by bioassay when the drug was administered intravenously. However, after oral administration the drug could be detected only by radioimmunoassay. The antiserum was evaluated for cross-reactivity with a wide variety of antibiotics and cancer chemotherapeutic agents, and no significant interference was found.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (809K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ANDERSON RC, WORTH HM, HARRIS PN, CHEN KK. Vancomycin, a new antibiotic. IV. Pharmacologic and toxicologic studies. Antibiot Annu. 1956:75–81. [PubMed]
  • Bodey GP, Rosenbaum B. Effect of prophylactic measures on the microbial flora of patients in protected environment units. Medicine (Baltimore) 1974 May;53(3):209–228. [PubMed]
  • Cook FV, Farrar WE., Jr Vancomycin revisited. Ann Intern Med. 1978 Jun;88(6):813–818. [PubMed]
  • Crossley KB, Rotschafer JC, Chern MM, Mead KE, Zaske DE. Comparison of a radioimmunoassay and a microbiological assay for measurement of serum vancomycin concentrations. Antimicrob Agents Chemother. 1980 Apr;17(4):654–657. [PMC free article] [PubMed]
  • GRIFFITH RS. Vancomycin; continued clinical studies. Antibiot Annu. 1956:118–122. [PubMed]
  • LEACH W. Ototoxicity of neomycin and other antibiotics. J Laryngol Otol. 1962 Oct;76:774–790. [PubMed]
  • LEE CC, ANDERSON RC, CHEN KK. Vancomycin, a new antibiotic. V. Distribution, excretion, and renal clearance. Antibiot Annu. 1956:82–89. [PubMed]
  • MARSHALL FJ. STRUCTURE STUDIES ON VANCOMYCIN. J Med Chem. 1965 Jan;8:18–22. [PubMed]
  • MCCORMICK MH, MCGUIRE JM, PITTENGER GE, PITTENGER RC, STARK WM. Vancomycin, a new antibiotic. I. Chemical and biologic properties. Antibiot Annu. 1955;3:606–611. [PubMed]
  • Sheldrick GM, Jones PG, Kennard O, Williams DH, Smith GA. Structure of vancomycin and its complex with acetyl-D-alanyl-D-alanine. Nature. 1978 Jan 19;271(5642):223–225. [PubMed]
  • Smith KA, Williams DH, Smith GA. Structural studies on the antibiotic vancomycin; the nature of the aromatic rings. J Chem Soc Perkin 1. 1974;(20):2369–2376. [PubMed]
  • Torres JR, Sanders CV, Lewis AC. Vancomycin concentration in human tissues--preliminary report. J Antimicrob Chemother. 1979 Jul;5(4):475–477. [PubMed]
  • Uhl JR, Anhalt JP. High performance liquid chromatographic assay of vancomycin in serum. Ther Drug Monit. 1979;1(1):75–83. [PubMed]
  • Walker CA, Kopp B. Sensitive bioassay for vancomycin. Antimicrob Agents Chemother. 1978 Jan;13(1):30–33. [PMC free article] [PubMed]
  • Weringa WD, Williams DH, Feeney J, Brown JP, King RW. The structure of an amino-sugar from the antibiotic vancomycin. J Chem Soc Perkin 1. 1972;3:443–446. [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...