• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Sep 1997; 179(18): 5878–5883.
PMCID: PMC179480

A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary-phase survival, stress adaptation, and expression of the gene encoding the stress protein UspA.

Abstract

An insertional mutation in ftsK, encoding an Escherichia coli product similar to the sporulation protein SpoIIIE of Bacillus subtilis, results in uspA overexpression in stationary phase and impairs cell division. The ftsK1::cat insertion mutant forms chains which are the result of inhibited cell-cell separation, while chromosome synthesis and partitioning appear to be normal as judged by flow cytometry and electron and light microscopy in combination with DNA staining. The cells of the chains are attached to each other by a small envelope structure, and unlike in a spoIIIE mutant of B. subtilis, there is no DNA trapped in the division plane. In addition, plasmids harboring a truncated ftsK allele lacking the last 195 bp of the gene cause chain formation in wild-type cells. While the mutant cells grow at essentially the same rate as the parent in complex and defined minimal media, they are sensitive to stresses. Specifically, the mutant failed to grow at elevated salt concentrations and survived stationary phase poorly. The phenotypes of the ftsK1::cat mutant are complemented by the 3' end (spoIIIE-like half) of the ftsK locus. In contrast, the 5' end of the ftsK locus reported to complement ftsK44(Ts) phenotypes does not complement the phenotypes of the ftsK1::cat mutant.

Full Text

The Full Text of this article is available as a PDF (1.7M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Albertson NH, Nyström T. Effects of starvation for exogenous carbon on functional mRNA stability and rate of peptide chain elongation in Escherichia coli. FEMS Microbiol Lett. 1994 Apr 1;117(2):181–187. [PubMed]
  • Beall B, Lutkenhaus J. FtsZ in Bacillus subtilis is required for vegetative septation and for asymmetric septation during sporulation. Genes Dev. 1991 Mar;5(3):447–455. [PubMed]
  • Beall B, Lutkenhaus J. Impaired cell division and sporulation of a Bacillus subtilis strain with the ftsA gene deleted. J Bacteriol. 1992 Apr;174(7):2398–2403. [PMC free article] [PubMed]
  • Begg KJ, Dewar SJ, Donachie WD. A new Escherichia coli cell division gene, ftsK. J Bacteriol. 1995 Nov;177(21):6211–6222. [PMC free article] [PubMed]
  • Bender J, Kleckner N. Genetic evidence that Tn10 transposes by a nonreplicative mechanism. Cell. 1986 Jun 20;45(6):801–815. [PubMed]
  • Churchward G, Belin D, Nagamine Y. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene. 1984 Nov;31(1-3):165–171. [PubMed]
  • Deuerling E, Mogk A, Richter C, Purucker M, Schumann W. The ftsH gene of Bacillus subtilis is involved in major cellular processes such as sporulation, stress adaptation and secretion. Mol Microbiol. 1997 Mar;23(5):921–933. [PubMed]
  • Dijkstra AJ, Keck W. Peptidoglycan as a barrier to transenvelope transport. J Bacteriol. 1996 Oct;178(19):5555–5562. [PMC free article] [PubMed]
  • Elliott T, Roth JR. Characterization of Tn10d-Cam: a transposition-defective Tn10 specifying chloramphenicol resistance. Mol Gen Genet. 1988 Aug;213(2-3):332–338. [PubMed]
  • Farewell A, Diez AA, DiRusso CC, Nyström T. Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes. J Bacteriol. 1996 Nov;178(22):6443–6450. [PMC free article] [PubMed]
  • Fung JC, MacAlister TJ, Weigand RA, Rothfield LI. Morphogenesis of the bacterial division septum: identification of potential sites of division in lkyD mutants of Salmonella typhimurium. J Bacteriol. 1980 Aug;143(2):1019–1024. [PMC free article] [PubMed]
  • Ichikawa JK, Li C, Fu J, Clarke S. A gene at 59 minutes on the Escherichia coli chromosome encodes a lipoprotein with unusual amino acid repeat sequences. J Bacteriol. 1994 Mar;176(6):1630–1638. [PMC free article] [PubMed]
  • Jenkins DE, Auger EA, Matin A. Role of RpoH, a heat shock regulator protein, in Escherichia coli carbon starvation protein synthesis and survival. J Bacteriol. 1991 Mar;173(6):1992–1996. [PMC free article] [PubMed]
  • Kleckner N, Bender J, Gottesman S. Uses of transposons with emphasis on Tn10. Methods Enzymol. 1991;204:139–180. [PubMed]
  • Lange R, Hengge-Aronis R. Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol Microbiol. 1991 Jan;5(1):49–59. [PubMed]
  • Levin PA, Losick R. Characterization of a cell division gene from Bacillus subtilis that is required for vegetative and sporulation septum formation. J Bacteriol. 1994 Mar;176(5):1451–1459. [PMC free article] [PubMed]
  • Li C, Clarke S. A protein methyltransferase specific for altered aspartyl residues is important in Escherichia coli stationary-phase survival and heat-shock resistance. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9885–9889. [PMC free article] [PubMed]
  • McCann MP, Kidwell JP, Matin A. The putative sigma factor KatF has a central role in development of starvation-mediated general resistance in Escherichia coli. J Bacteriol. 1991 Jul;173(13):4188–4194. [PMC free article] [PubMed]
  • Moller S, Kristensen CS, Poulsen LK, Carstensen JM, Molin S. Bacterial growth on surfaces: automated image analysis for quantification of growth rate-related parameters. Appl Environ Microbiol. 1995 Feb;61(2):741–748. [PMC free article] [PubMed]
  • Nyström T. The glucose-starvation stimulon of Escherichia coli: induced and repressed synthesis of enzymes of central metabolic pathways and role of acetyl phosphate in gene expression and starvation survival. Mol Microbiol. 1994 Jun;12(5):833–843. [PubMed]
  • Nystöm T. Role of guanosine tetraphosphate in gene expression and the survival of glucose or seryl-tRNA starved cells of Escherichia coli K12. Mol Gen Genet. 1994 Nov 1;245(3):355–362. [PubMed]
  • Nyström T. Glucose starvation stimulon of Escherichia coli: role of integration host factor in starvation survival and growth phase-dependent protein synthesis. J Bacteriol. 1995 Oct;177(19):5707–5710. [PMC free article] [PubMed]
  • Nyström T. The trials and tribulations of growth arrest. Trends Microbiol. 1995 Apr;3(4):131–136. [PubMed]
  • Nyström T, Neidhardt FC. Cloning, mapping and nucleotide sequencing of a gene encoding a universal stress protein in Escherichia coli. Mol Microbiol. 1992 Nov;6(21):3187–3198. [PubMed]
  • Nyström T, Neidhardt FC. Isolation and properties of a mutant of Escherichia coli with an insertional inactivation of the uspA gene, which encodes a universal stress protein. J Bacteriol. 1993 Jul;175(13):3949–3956. [PMC free article] [PubMed]
  • Nyström T, Neidhardt FC. Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest. Mol Microbiol. 1994 Feb;11(3):537–544. [PubMed]
  • Nyström T, Neidhardt FC. Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12. J Bacteriol. 1996 Feb;178(3):927–930. [PMC free article] [PubMed]
  • Nyström T, Larsson C, Gustafsson L. Bacterial defense against aging: role of the Escherichia coli ArcA regulator in gene expression, readjusted energy flux and survival during stasis. EMBO J. 1996 Jul 1;15(13):3219–3228. [PMC free article] [PubMed]
  • Spence J, Cegielska A, Georgopoulos C. Role of Escherichia coli heat shock proteins DnaK and HtpG (C62.5) in response to nutritional deprivation. J Bacteriol. 1990 Dec;172(12):7157–7166. [PMC free article] [PubMed]
  • Tomoyasu T, Gamer J, Bukau B, Kanemori M, Mori H, Rutman AJ, Oppenheim AB, Yura T, Yamanaka K, Niki H, et al. Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heat-shock transcription factor sigma 32. EMBO J. 1995 Jun 1;14(11):2551–2560. [PMC free article] [PubMed]
  • Tormo A, Almirón M, Kolter R. surA, an Escherichia coli gene essential for survival in stationary phase. J Bacteriol. 1990 Aug;172(8):4339–4347. [PMC free article] [PubMed]
  • Vanderwinkel E, De Vlieghere M, Fontaine M, Charles D, Denamur F, Vandevoorde D, De Kegel D. Septation deficiency and phosphilipid perturbation in Escherichia coli genetically constitutive for the beta oxidation pathway. J Bacteriol. 1976 Sep;127(3):1389–1399. [PMC free article] [PubMed]
  • Wanner BL. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J Mol Biol. 1986 Sep 5;191(1):39–58. [PubMed]
  • Wu LJ, Errington J. Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division. Science. 1994 Apr 22;264(5158):572–575. [PubMed]
  • Wu LJ, Lewis PJ, Allmansberger R, Hauser PM, Errington J. A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis. Genes Dev. 1995 Jun 1;9(11):1316–1326. [PubMed]
  • Young K, Silver LL, Bramhill D, Cameron P, Eveland SS, Raetz CR, Hyland SA, Anderson MS. The envA permeability/cell division gene of Escherichia coli encodes the second enzyme of lipid A biosynthesis. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase. J Biol Chem. 1995 Dec 22;270(51):30384–30391. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...