• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of archdischArchives of Disease in ChildhoodVisit this articleSubmit a manuscriptReceive email alertsContact usBMJ
Arch Dis Child. Aug 1991; 66(8): 941–947.
PMCID: PMC1793455

Antigliadin and antiendomysium antibody determination for coeliac disease.

Abstract

The value of IgG and IgA gliadin antibodies (AGA) was compared with that of IgA endomysium antibodies (EMA) for the diagnosis of coeliac disease. Three hundred and six of 340 (90%) children with untreated coeliac disease (flat mucosa) had EMA and 338/340 (99.4%) had IgG AGA and/or IgA AGA. Only 1/340 (a 7 year old boy with selective IgA deficiency) had neither AGA nor EMA. Absence of EMA is more frequent in coeliac patients younger than 2 years than in older patients (32/277 compared with 1/62). EMA were present in 4/211 (2%) of comparison subjects (normal mucosa), IgA AGA in 12/211 (6%), and IgG AGA in 74/211 (35%). The specificity of AGA cannot be calculated from these figures as they are biased. The combined determination of AGA and EMA, taking advantage of the high sensitivity of AGA and the high specificity of EMA, gives an excellent prediction of the condition of the mucosa: 247/248 patients (99.6%) with positive EMA and positive IgG AGA and IgA AGA had a flat mucosa, whereas 136/137 patients (99.3%) with neither AGA nor EMA had a normal mucosa. During a gluten free diet EMA and AGA disappear. Their presence or absence is therefore an indicator of dietary compliance. After reintroduction of gluten into the diet 110/134 (82%) of the patients who had a flat mucosa at diagnosis relapsed, but 24/134 still had a normal mucosa after 2-15 years of challenge. All these patients without a morphological relapse were less than 2 years old at diagnosis so we conclude that patients who are young at diagnosis should be challenged. AGA often reappear earlier than EMA. After one month of challenge 93% of patients are AGA and 69% EMA positive. After more than three years of gluten intake the percentage of AGA positive patients decreased to about 50% whereas the percentage of EMA positive sera was then highest (93%). Therefore EMA are more sensitive for the detection of 'silent' relapse after prolonged periods of gluten intake.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Burgin-Wolf A, Hernandez R, Just M. A rapid fluorescent solid-phase method for detecting antibodies against milk proteins and gliadin in different immunoglobulin classes. Experientia. 1972 Jan 15;28(1):119–120. [PubMed]
  • Bürgin-Wolff A, Hernandez R, Just M, Signer E. Immunofluorescent antibodies against gliadin: a screening test for coeliac disease. Helv Paediatr Acta. 1976 Dec;31(4-5):375–380. [PubMed]
  • Bürgin-Wolff A, Bertele RM, Berger R, Gaze H, Harms HK, Just M, Khanna S, Schürmann K, Signer E, Tomovic D. A reliable screening test for childhood celiac disease: fluorescent immunosorbent test for gliadin antibodies. A prospective multicenter study. J Pediatr. 1983 May;102(5):655–660. [PubMed]
  • Bürgin-Wolff A, Berger R, Gaze H, Huber H, Lentze MJ, Nusslé D. IgG, IgA and IgE gliadin antibody determinations as screening test for untreated coeliac disease in children, a multicentre study. Eur J Pediatr. 1989 Apr;148(6):496–502. [PubMed]
  • Signer E, Bürgin-Wolff A, Berger R, Birbaumer A, Just M. Antibodies to gliadin as a screening test for coeliac disease. A prospective study. Helv Paediatr Acta. 1979 Feb;34(1):41–52. [PubMed]
  • Blazer S, Naveh Y, Berant M, Merzbach D, Sperber S. Serum IgG antibodies to gliadin in children with celiac disease as measured by an immunofluorescence method. J Pediatr Gastroenterol Nutr. 1984 Mar;3(2):205–209. [PubMed]
  • Juto P, Fredrikzon B, Hernell O. Gliadin-specific serum immunoglobulins A, E, G, and M in childhood: relation to small intestine mucosal morphology. J Pediatr Gastroenterol Nutr. 1985 Oct;4(5):723–729. [PubMed]
  • Kelly J, O'Farrelly C, Rees JP, Feighery C, Weir DG. Humoral response to alpha gliadin as serological screening test for coeliac disease. Arch Dis Child. 1987 May;62(5):469–473. [PMC free article] [PubMed]
  • Kieffer M. Serum antibodies to gliadin and other cereal proteins in patients with coeliac disease and dermatitis herpetiformis. Dan Med Bull. 1985 Oct;32(5):251–262. [PubMed]
  • Koninckx CR, Giliams JP, Polanco I, Peña AS. IgA antigliadin antibodies in celiac and inflammatory bowel disease. J Pediatr Gastroenterol Nutr. 1984 Nov;3(5):676–682. [PubMed]
  • Scott H, Fausa O, Ek J, Brandtzaeg P. Immune response patterns in coeliac disease. Serum antibodies to dietary antigens measured by an enzyme linked immunosorbent assay (ELISA). Clin Exp Immunol. 1984 Jul;57(1):25–32. [PMC free article] [PubMed]
  • Stenhammar L, Kilander AF, Nilsson LA, Strömberg L, Tarkowski A. Serum gliadin antibodies for detection and control of childhood coeliac disease. Acta Paediatr Scand. 1984 Sep;73(5):657–663. [PubMed]
  • Troncone R, Pignata C, Farris E, Ciccimarra F. A solid-phase radioimmunoassay for IgG gliadin antibodies using 125I-labelled staphylococcal protein A. J Immunol Methods. 1983 Oct 14;63(2):163–170. [PubMed]
  • Volta U, Lenzi M, Lazzari R, Cassani F, Collina A, Bianchi FB, Pisi E. Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease. Gut. 1985 Jul;26(7):667–671. [PMC free article] [PubMed]
  • Auricchio S, Greco L, Troncone R. Gluten-sensitive enteropathy in childhood. Pediatr Clin North Am. 1988 Feb;35(1):157–187. [PubMed]
  • Guandalini S, Ventura A, Ansaldi N, Giunta AM, Greco L, Lazzari R, Mastella G, Rubino A. Diagnosis of coeliac disease: time for a change? Arch Dis Child. 1989 Sep;64(9):1320–1325. [PMC free article] [PubMed]
  • Begg CB, Greenes RA. Assessment of diagnostic tests when disease verification is subject to selection bias. Biometrics. 1983 Mar;39(1):207–215. [PubMed]
  • Troncone R, Ferguson A. Anti-gliadin antibodies. J Pediatr Gastroenterol Nutr. 1991 Feb;12(2):150–158. [PubMed]
  • Scott H, Brandtzaeg P. Gluten IgA antibodies and coeliac disease. Lancet. 1989 Feb 18;1(8634):382–383. [PubMed]
  • Chorzelski TP, Beutner EH, Sulej J, Tchorzewska H, Jablonska S, Kumar V, Kapuscinska A. IgA anti-endomysium antibody. A new immunological marker of dermatitis herpetiformis and coeliac disease. Br J Dermatol. 1984 Oct;111(4):395–402. [PubMed]
  • Beutner EH, Kumar V, Chorzelski TP, Szaflarska-Czerwionka M. IgG endomysial antibodies in IgA-deficient patient with coeliac disease. Lancet. 1989 Jun 3;1(8649):1261–1262. [PubMed]
  • Rossi TM, Kumar V, Lerner A, Heitlinger LA, Tucker N, Fisher J. Relationship of endomysial antibodies to jejunal mucosal pathology: specificity towards both symptomatic and asymptomatic celiacs. J Pediatr Gastroenterol Nutr. 1988 Nov-Dec;7(6):858–863. [PubMed]
  • Scott H, Ek J, Brandtzaeg P. Changes of serum antibody activities to various dietary antigens related to gluten withdrawal or challenge in children with coeliac disease. Int Arch Allergy Appl Immunol. 1985;76(2):138–144. [PubMed]
  • Mäki M, Lähdeaho ML, Hällström O, Viander M, Visakorpi JK. Postpubertal gluten challenge in coeliac disease. Arch Dis Child. 1989 Nov;64(11):1604–1607. [PMC free article] [PubMed]
  • Kárpáti S, Bürgin-Wolff A, Krieg T, Meurer M, Stolz W, Braun-Falco O. Binding to human jejunum of serum IgA antibody from children with coeliac disease. Lancet. 1990 Dec 1;336(8727):1335–1338. [PubMed]
  • Malcolm S, Clayton-Smith J, Nichols M, Robb S, Webb T, Armour JA, Jeffreys AJ, Pembrey ME. Uniparental paternal disomy in Angelman's syndrome. Lancet. 1991 Mar 23;337(8743):694–697. [PubMed]
  • Nicholls RD, Knoll JH, Butler MG, Karam S, Lalande M. Genetic imprinting suggested by maternal heterodisomy in nondeletion Prader-Willi syndrome. Nature. 1989 Nov 16;342(6247):281–285. [PubMed]

Articles from Archives of Disease in Childhood are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • Compound
    Compound
    PubChem Compound links
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...