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J Bacteriol. Jul 1997; 179(14): 4591–4598.
PMCID: PMC179296

Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.

Abstract

Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analysis suggested that an inositol-inducible promoter for the iol operon (iol promoter) might be a promoter-like sequence in the 5' region of iolA, which is probably recognized by sigmaA. S1 nuclease analysis implied that a rho-independent terminator-like structure in the 3' region of iolJ might be a terminator for iol transcription. Disruption of the iol promoter prevented synthesis of the iol transcript as well as that of Idh, implying that the iol operon is most probably transcribed as an 11.5-kb mRNA containing the 10 iol genes. Immediately upstream of the iol operon, two genes (iolR and iolS) with divergent orientations to the iol operon were found. Disruption of iolR (but not iolS) caused constitutive synthesis of the iol transcript and Idh, indicating that the iolR gene encodes a transcription-negative regulator (presumably a repressor) for the iol operon. Northern and S1 nuclease analyses revealed that the iolRS genes were cotranscribed from another inositol-inducible promoter, which is probably recognized by sigmaA. The promoter assignments of the iol and iolRS operons were confirmed in vivo with a lacZ fusion integrated into the amyE locus.

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Selected References

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  • Anderson WA, Magasanik B. The pathway of myo-inositol degradation in Aerobacter aerogenes. Identification of the intermediate 2-deoxy-5-keto-D-gluconic acid. J Biol Chem. 1971 Sep 25;246(18):5653–5661. [PubMed]
  • Anderson WA, Magasanik B. The pathway of myo-inositol degradation in Aerobacter aerogenes. Conversion of 2-deoxy-5-keto-D-gluconic acid to glycolytic intermediates. J Biol Chem. 1971 Sep 25;246(18):5662–5675. [PubMed]
  • Atkinson MR, Wray LV, Jr, Fisher SH. Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis. J Bacteriol. 1990 Sep;172(9):4758–4765. [PMC free article] [PubMed]
  • Berman T, Magasanik B. The pathway of myo-inositol degradation in Aerobacter aerogenes. Dehydrogenation and dehydration. J Biol Chem. 1966 Feb 25;241(4):800–806. [PubMed]
  • Berman T, Magasanik B. The pathway of myo-inositol degradation in Aerobacter aerogenes. Ring scission. J Biol Chem. 1966 Feb 25;241(4):807–813. [PubMed]
  • Deutscher J, Reizer J, Fischer C, Galinier A, Saier MH, Jr, Steinmetz M. Loss of protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis. J Bacteriol. 1994 Jun;176(11):3336–3344. [PMC free article] [PubMed]
  • Fujita Y, Freese E. Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase. J Bacteriol. 1981 Feb;145(2):760–767. [PMC free article] [PubMed]
  • Fujita Y, Fujita T. Genetic analysis of a pleiotropic deletion mutation (delta igf) in Bacillus subtilis. J Bacteriol. 1983 May;154(2):864–869. [PMC free article] [PubMed]
  • Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y. Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis. J Biol Chem. 1986 Oct 15;261(29):13744–13753. [PubMed]
  • Fujita Y, Miwa Y, Galinier A, Deutscher J. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr. Mol Microbiol. 1995 Sep;17(5):953–960. [PubMed]
  • Fujita Y, Shindo K, Miwa Y, Yoshida K. Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli. Gene. 1991 Dec 1;108(1):121–125. [PubMed]
  • Miwa Y, Saikawa M, Fujita Y. Possible function and some properties of the CcpA protein of Bacillus subtilis. Microbiology. 1994 Oct;140(Pt 10):2567–2575. [PubMed]
  • Nihashi J, Fujita Y. Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis. Biochim Biophys Acta. 1984 Mar 22;798(1):88–95. [PubMed]
  • Pearson WR, Lipman DJ. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. [PMC free article] [PubMed]
  • Ramaley R, Fujita Y, Freese E. Purification and properties of Bacillus subtilis inositol dehydrogenase. J Biol Chem. 1979 Aug 25;254(16):7684–7690. [PubMed]
  • Shimotsu H, Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene. 1986;43(1-2):85–94. [PubMed]
  • Vidal-Leiria M, van Uden N. Inositol dehydrogenase from the yeast Cryptococcus melibiosum. Biochim Biophys Acta. 1973 Feb 15;293(2):295–303. [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Yoshida K, Sano H, Miwa Y, Ogasawara N, Fujita Y. Cloning and nucleotide sequencing of a 15 kb region of the Bacillus subtilis genome containing the iol operon. Microbiology. 1994 Sep;140(Pt 9):2289–2298. [PubMed]
  • Yoshida K, Seki S, Fujimura M, Miwa Y, Fujita Y. Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome between the gnt and iol operons. DNA Res. 1995;2(2):61–69. [PubMed]
  • Yoshida K, Shindo K, Sano H, Seki S, Fujimura M, Yanai N, Miwa Y, Fujita Y. Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region. Microbiology. 1996 Nov;142(Pt 11):3113–3123. [PubMed]

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