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J Bacteriol. Jul 1997; 179(13): 4299–4304.
PMCID: PMC179253

Aerobic regulation of isocitrate dehydrogenase gene (icd) expression in Escherichia coli by the arcA and fnr gene products.


Isocitrate dehydrogenase, the icd gene product, has been studied extensively regarding the regulation of enzymatic activity and its relationship to the metabolic flux between the tricarboxylic acid cycle and the glyoxylate bypass. In this study, the transcriptional regulation of icd gene expression was monitored by using an icd-lacZ gene fusion and shown to vary over a 15-fold range in response to changes in oxygen and carbon availability. Anaerobic cell growth resulted in fivefold-lower icd-lacZ expression than during aerobic growth. This negative control is mediated by the arcA and fnr gene products. When different carbon compounds were used for cell growth, icd-lacZ expression varied threefold. The results of continuous cell culture studies indicated that this control may be due to variations in cell growth rate rather than to catabolite repression. DNase I footprinting at the icd promoter revealed a 42-bp ArcA-phosphate-protected region that overlaps the start site of icd transcription. Phosphorylation of ArcA considerably enhanced its binding to DNA, while ArcA-phosphate exhibited an apparent dissociation value of approximately 0.1 microM. Based on these studies, ArcA appears to function as a classical repressor of transcription by binding at a site overlapping the icd promoter during anaerobic cell growth conditions.

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Selected References

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  • Compan I, Touati D. Anaerobic activation of arcA transcription in Escherichia coli: roles of Fnr and ArcA. Mol Microbiol. 1994 Mar;11(5):955–964. [PubMed]
  • Cotter PA, Gunsalus RP. Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli. J Bacteriol. 1989 Jul;171(7):3817–3823. [PMC free article] [PubMed]
  • Drapal N, Sawers G. Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region. Mol Microbiol. 1995 May;16(3):597–607. [PubMed]
  • el-Mansi EM, Dawson GC, Bryce CF. Steady-state modelling of metabolic flux between the tricarboxylic acid cycle and the glyoxylate bypass in Escherichia coli. Comput Appl Biosci. 1994 Jun;10(3):295–299. [PubMed]
  • Goosen N, van de Putte P. The regulation of transcription initiation by integration host factor. Mol Microbiol. 1995 Apr;16(1):1–7. [PubMed]
  • Gray CT, Wimpenny JW, Mossman MR. Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli. Biochim Biophys Acta. 1966 Mar 28;117(1):33–41. [PubMed]
  • Gruer MJ, Guest JR. Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli. Microbiology. 1994 Oct;140(Pt 10):2531–2541. [PubMed]
  • Gunsalus RP, Park SJ. Aerobic-anaerobic gene regulation in Escherichia coli: control by the ArcAB and Fnr regulons. Res Microbiol. 1994 Jun-Aug;145(5-6):437–450. [PubMed]
  • Iuchi S, Aristarkhov A, Dong JM, Taylor JS, Lin EC. Effects of nitrate respiration on expression of the Arc-controlled operons encoding succinate dehydrogenase and flavin-linked L-lactate dehydrogenase. J Bacteriol. 1994 Mar;176(6):1695–1701. [PMC free article] [PubMed]
  • Iuchi S, Lin EC. arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1888–1892. [PMC free article] [PubMed]
  • Kolesnikow T, Schröder I, Gunsalus RP. Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. J Bacteriol. 1992 Nov;174(22):7104–7111. [PMC free article] [PubMed]
  • Kornberg HL. The role and control of the glyoxylate cycle in Escherichia coli. Biochem J. 1966 Apr;99(1):1–11. [PMC free article] [PubMed]
  • LaPorte DC, Chung T. A single gene codes for the kinase and phosphatase which regulate isocitrate dehydrogenase. J Biol Chem. 1985 Dec 5;260(28):15291–15297. [PubMed]
  • LaPorte DC, Koshland DE., Jr A protein with kinase and phosphatase activities involved in regulation of tricarboxylic acid cycle. Nature. 1982 Dec 2;300(5891):458–460. [PubMed]
  • Lynch AS, Lin EC. Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters. J Bacteriol. 1996 Nov;178(21):6238–6249. [PMC free article] [PubMed]
  • Park SJ, Cotter PA, Gunsalus RP. Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability. J Bacteriol. 1995 Nov;177(22):6652–6656. [PMC free article] [PubMed]
  • Park SJ, Gunsalus RP. Oxygen, iron, carbon, and superoxide control of the fumarase fumA and fumC genes of Escherichia coli: role of the arcA, fnr, and soxR gene products. J Bacteriol. 1995 Nov;177(21):6255–6262. [PMC free article] [PubMed]
  • Park SJ, McCabe J, Turna J, Gunsalus RP. Regulation of the citrate synthase (gltA) gene of Escherichia coli in response to anaerobiosis and carbon supply: role of the arcA gene product. J Bacteriol. 1994 Aug;176(16):5086–5092. [PMC free article] [PubMed]
  • Park SJ, Tseng CP, Gunsalus RP. Regulation of succinate dehydrogenase (sdhCDAB) operon expression in Escherichia coli in response to carbon supply and anaerobiosis: role of ArcA and Fnr. Mol Microbiol. 1995 Feb;15(3):473–482. [PubMed]
  • Prentki P, Chandler M, Galas DJ. Escherichia coli integration host factor bends the DNA at the ends of IS1 and in an insertion hotspot with multiple IHF binding sites. EMBO J. 1987 Aug;6(8):2479–2487. [PMC free article] [PubMed]
  • Schröder I, Darie S, Gunsalus RP. Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. J Biol Chem. 1993 Jan 15;268(2):771–774. [PubMed]
  • Simons RW, Houman F, Kleckner N. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene. 1987;53(1):85–96. [PubMed]
  • Spiro S, Guest JR. FNR and its role in oxygen-regulated gene expression in Escherichia coli. FEMS Microbiol Rev. 1990 Aug;6(4):399–428. [PubMed]
  • Tardat B, Touati D. Iron and oxygen regulation of Escherichia coli MnSOD expression: competition between the global regulators Fur and ArcA for binding to DNA. Mol Microbiol. 1993 Jul;9(1):53–63. [PubMed]
  • Thorsness PE, Koshland DE., Jr Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate. J Biol Chem. 1987 Aug 5;262(22):10422–10425. [PubMed]
  • Tseng CP, Hansen AK, Cotter P, Gunsalus RP. Effect of cell growth rate on expression of the anaerobic respiratory pathway operons frdABCD, dmsABC, and narGHJI of Escherichia coli. J Bacteriol. 1994 Nov;176(21):6599–6605. [PMC free article] [PubMed]
  • Walsh K, Koshland DE., Jr Branch point control by the phosphorylation state of isocitrate dehydrogenase. A quantitative examination of fluxes during a regulatory transition. J Biol Chem. 1985 Jul 15;260(14):8430–8437. [PubMed]

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