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J Bacteriol. 1997 May; 179(10): 3244–3249.
PMCID: PMC179103

Genome structure and phylogeny in the genus Brucella.


PacI and SpeI restriction maps were obtained for the two chromosomes of each of the six species of the genus Brucella: B. melitensis, B. abortus, B. suis, B. canis, B. ovis, and B. neotomae. Three complementary techniques were used: hybridization with the two replicons as probes, cross-hybridization of restriction fragments, and a new mapping method. For each type strain, a unique I-SceI site was introduced in each of the two replicons, and the location of SpeI sites was determined by linearization at the unique site, partial digestion, and end labeling of the fragments. The restriction and genetic maps of the six species were highly conserved. However, numerous small insertions or deletions, ranging from 1 to 34 kb, were observed by comparison with the map of the reference strain of the genus, B. melitensis 16M. A 21-kb Spel fragment specific to B. ovis was found in the small chromosome of this species. A 640-kb inversion was demonstrated in the B. abortus small chromosome. All of these data allowed the construction of a phylogenetic tree, which reflects the traditional phenetic classification of the genus.

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Selected References

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  • Allardet-Servent A, Bourg G, Ramuz M, Pages M, Bellis M, Roizes G. DNA polymorphism in strains of the genus Brucella. J Bacteriol. 1988 Oct;170(10):4603–4607. [PMC free article] [PubMed]
  • Canard B, Saint-Joanis B, Cole ST. Genomic diversity and organization of virulence genes in the pathogenic anaerobe Clostridium perfringens. Mol Microbiol. 1992 Jun;6(11):1421–1429. [PubMed]
  • Carlson CR, Caugant DA, Kolstø AB. Genotypic Diversity among Bacillus cereus and Bacillus thuringiensis Strains. Appl Environ Microbiol. 1994 Jun;60(6):1719–1725. [PMC free article] [PubMed]
  • Casjens S, Delange M, Ley HL, 3rd, Rosa P, Huang WM. Linear chromosomes of Lyme disease agent spirochetes: genetic diversity and conservation of gene order. J Bacteriol. 1995 May;177(10):2769–2780. [PMC free article] [PubMed]
  • Colleaux L, Rougeulle C, Avner P, Dujon B. Rapid physical mapping of YAC inserts by random integration of I-Sce I sites. Hum Mol Genet. 1993 Mar;2(3):265–271. [PubMed]
  • Daniels DL. The complete AvrII restriction map of the Escherichia coli genome and comparisons of several laboratory strains. Nucleic Acids Res. 1990 May 11;18(9):2649–2651. [PMC free article] [PubMed]
  • Denoel PA, Zygmunt MS, Weynants V, Tibor A, Lichtfouse B, Briffeuil P, Limet JN, Letesson JJ. Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain. FEBS Lett. 1995 Mar 20;361(2-3):238–242. [PubMed]
  • Ficht TA, Husseinen HS, Derr J, Bearden SW. Species-specific sequences at the omp2 locus of Brucella type strains. Int J Syst Bacteriol. 1996 Jan;46(1):329–331. [PubMed]
  • Fitch WM, Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. [PubMed]
  • Fonstein M, Nikolskaya T, Haselkorn R. High-resolution alignment of a 1-megabase-long genome region of three strains of Rhodobacter capsulatus. J Bacteriol. 1995 May;177(9):2368–2372. [PMC free article] [PubMed]
  • Grothues D, Tümmler B. New approaches in genome analysis by pulsed-field gel electrophoresis: application to the analysis of Pseudomonas species. Mol Microbiol. 1991 Nov;5(11):2763–2776. [PubMed]
  • Halling SM, Bricker BJ. Characterization and occurrence of two repeated palindromic DNA elements of Brucella spp.: Bru-RS1 and Bru-RS2. Mol Microbiol. 1994 Nov;14(4):681–689. [PubMed]
  • Halling SM, Tatum FM, Bricker BJ. Sequence and characterization of an insertion sequence, IS711, from Brucella ovis. Gene. 1993 Oct 29;133(1):123–127. [PubMed]
  • Hemmen F, Weynants V, Scarcez T, Letesson JJ, Saman E. Cloning and sequence analysis of a newly identified Brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes. Clin Diagn Lab Immunol. 1995 May;2(3):263–267. [PMC free article] [PubMed]
  • Hill CW, Harnish BW. Inversions between ribosomal RNA genes of Escherichia coli. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7069–7072. [PMC free article] [PubMed]
  • Hiller B, Frey B, Schumann W. Tn 5Map, a transposon for the rapid mapping of restriction sites in plasmids. FEMS Microbiol Lett. 1994 Jan 15;115(2-3):151–155. [PubMed]
  • Jiang Q, Hiratsuka K, Taylor DE. Variability of gene order in different Helicobacter pylori strains contributes to genome diversity. Mol Microbiol. 1996 May;20(4):833–842. [PubMed]
  • Jumas-Bilak E, Maugard C, Michaux-Charachon S, Allardet-Servent A, Perrin A, O'Callaghan D, Ramuz M. Study of the organization of the genomes of Escherichia coli, Brucella melitensis and Agrobacterium tumefaciens by insertion of a unique restriction site. Microbiology. 1995 Oct;141(Pt 10):2425–2432. [PubMed]
  • Komoda Y, Enomoto M, Tominaga A. Large inversion in Escherichia coli K-12 1485IN between inversely oriented IS3 elements near lac and cdd. Genetics. 1991 Nov;129(3):639–645. [PMC free article] [PubMed]
  • Ladefoged SA, Christiansen G. Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis. J Bacteriol. 1992 Apr;174(7):2199–2207. [PMC free article] [PubMed]
  • Leblond P, Redenbach M, Cullum J. Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol. 1993 Jun;175(11):3422–3429. [PMC free article] [PubMed]
  • Le Bourgeois P, Lautier M, Mata M, Ritzenthaler P. Physical and genetic map of the chromosome of Lactococcus lactis subsp. lactis IL1403. J Bacteriol. 1992 Nov;174(21):6752–6762. [PMC free article] [PubMed]
  • Le Bourgeois P, Lautier M, van den Berghe L, Gasson MJ, Ritzenthaler P. Physical and genetic map of the Lactococcus lactis subsp. cremoris MG1363 chromosome: comparison with that of Lactococcus lactis subsp. lactis IL 1403 reveals a large genome inversion. J Bacteriol. 1995 May;177(10):2840–2850. [PMC free article] [PubMed]
  • Liu SL, Hessel A, Sanderson KE. The XbaI-BlnI-CeuI genomic cleavage map of Salmonella enteritidis shows an inversion relative to Salmonella typhimurium LT2. Mol Microbiol. 1993 Nov;10(3):655–664. [PubMed]
  • Liu SL, Sanderson KE. Rearrangements in the genome of the bacterium Salmonella typhi. Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1018–1022. [PMC free article] [PubMed]
  • Liu SL, Sanderson KE. The chromosome of Salmonella paratyphi A is inverted by recombination between rrnH and rrnG. J Bacteriol. 1995 Nov;177(22):6585–6592. [PMC free article] [PubMed]
  • Margolin W, Bramhill D, Long SR. The dnaA gene of Rhizobium meliloti lies within an unusual gene arrangement. J Bacteriol. 1995 May;177(10):2892–2900. [PMC free article] [PubMed]
  • Michaux S, Paillisson J, Carles-Nurit MJ, Bourg G, Allardet-Servent A, Ramuz M. Presence of two independent chromosomes in the Brucella melitensis 16M genome. J Bacteriol. 1993 Feb;175(3):701–705. [PMC free article] [PubMed]
  • Monteilhet C, Perrin A, Thierry A, Colleaux L, Dujon B. Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. Nucleic Acids Res. 1990 Mar 25;18(6):1407–1413. [PMC free article] [PubMed]
  • Ojaimi C, Davidson BE, Saint Girons I, Old IG. Conservation of gene arrangement and an unusual organization of rRNA genes in the linear chromosomes of the Lyme disease spirochaetes Borrelia burgdorferi, B. garinii and B. afzelii. Microbiology. 1994 Nov;140(Pt 11):2931–2940. [PubMed]
  • Ouahrani S, Michaux S, Sri Widada J, Bourg G, Tournebize R, Ramuz M, Liautard JP. Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies. J Gen Microbiol. 1993 Dec;139(12):3265–3273. [PubMed]
  • Perkins JD, Heath JD, Sharma BR, Weinstock GM. XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. J Mol Biol. 1993 Jul 20;232(2):419–445. [PubMed]
  • Sangari FJ, García-Lobo JM, Agüero J. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol Lett. 1994 Sep 1;121(3):337–342. [PubMed]
  • Smith HO, Birnstiel ML. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. [PMC free article] [PubMed]
  • Tatum FM, Cheville NF, Morfitt D. Cloning, characterization and construction of htrA and htrA-like mutants of Brucella abortus and their survival in BALB/c mice. Microb Pathog. 1994 Jul;17(1):23–36. [PubMed]
  • Tatum FM, Morfitt DC, Halling SM. Construction of a Brucella abortus RecA mutant and its survival in mice. Microb Pathog. 1993 Mar;14(3):177–185. [PubMed]
  • Thierry A, Dujon B. Nested chromosomal fragmentation in yeast using the meganuclease I-Sce I: a new method for physical mapping of eukaryotic genomes. Nucleic Acids Res. 1992 Nov 11;20(21):5625–5631. [PMC free article] [PubMed]
  • Tibor A, Weynants V, Denoel P, Lichtfouse B, De Bolle X, Saman E, Limet JN, Letesson JJ. Molecular cloning, nucleotide sequence, and occurrence of a 16.5-kilodalton outer membrane protein of Brucella abortus with similarity to pal lipoproteins. Infect Immun. 1994 Sep;62(9):3633–3639. [PMC free article] [PubMed]
  • Zuerner RL, Herrmann JL, Saint Girons I. Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity. J Bacteriol. 1993 Sep;175(17):5445–5451. [PMC free article] [PubMed]

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