• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Apr 1997; 179(7): 2452–2458.
PMCID: PMC178989

The Agrobacterium tumefaciens virulence gene chvE is part of a putative ABC-type sugar transport operon.

Abstract

The Agrobacterium tumefaciens virulence determinant ChvE is a periplasmic binding protein which participates in chemotaxis and virulence gene induction in response to monosaccharides which occur in the plant wound environment. The region downstream of the A. tumefaciens chvE gene was cloned and sequenced for nucleotide and expression analysis. Three open reading frames transcribed in the same direction as chvE were revealed. The first two, together with chvE, encode putative proteins of a periplasmic binding protein-dependent sugar uptake system, or ABC-type (ATP binding cassette) transporter. The third open reading frame encodes a protein of unknown function. The deduced transporter gene products are related on the amino acid level to bacterial sugar transporters and probably function in glucose and galactose uptake. We have named these genes gguA, -B, and -C, for glucose galactose uptake. Mutations in gguA, gguB, or gguC do not affect virulence of A. tumefaciens on Kalanchoe diagremontiana; growth on 1 mM galactose, glucose, xylose, ribose, arabinose, fucose, or sucrose; or chemotaxis toward glucose, galactose, xylose, or arabinose.

Full Text

The Full Text of this article is available as a PDF (159K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Ankenbauer RG, Nester EW. Sugar-mediated induction of Agrobacterium tumefaciens virulence genes: structural specificity and activities of monosaccharides. J Bacteriol. 1990 Nov;172(11):6442–6446. [PMC free article] [PubMed]
  • Bardin S, Dan S, Osteras M, Finan TM. A phosphate transport system is required for symbiotic nitrogen fixation by Rhizobium meliloti. J Bacteriol. 1996 Aug;178(15):4540–4547. [PMC free article] [PubMed]
  • Bollinger J, Park C, Harayama S, Hazelbauer GL. Structure of the Trg protein: Homologies with and differences from other sensory transducers of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3287–3291. [PMC free article] [PubMed]
  • Cangelosi GA, Ankenbauer RG, Nester EW. Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6708–6712. [PMC free article] [PubMed]
  • Cangelosi GA, Martinetti G, Leigh JA, Lee CC, Thienes C, Theines C, Nester EW. Role for [corrected] Agrobacterium tumefaciens ChvA protein in export of beta-1,2-glucan. J Bacteriol. 1989 Mar;171(3):1609–1615. [PMC free article] [PubMed]
  • Charles TC, Nester EW. A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens. J Bacteriol. 1993 Oct;175(20):6614–6625. [PMC free article] [PubMed]
  • Chen CY, Winans SC. Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter. J Bacteriol. 1991 Feb;173(3):1139–1144. [PMC free article] [PubMed]
  • Chilton MD, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672–3676. [PMC free article] [PubMed]
  • Cornish A, Greenwood JA, Jones CW. Binding-protein-dependent sugar transport by Agrobacterium radiobacter and A. tumefaciens grown in continuous culture. J Gen Microbiol. 1989 Nov;135(11):3001–3013. [PubMed]
  • Das A, Stachel S, Ebert P, Allenza P, Montoya A, Nester E. Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes. Nucleic Acids Res. 1986 Feb 11;14(3):1355–1364. [PMC free article] [PubMed]
  • Doty SL, Chang M, Nester EW. The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member. J Bacteriol. 1993 Dec;175(24):7880–7886. [PMC free article] [PubMed]
  • Doty SL, Yu MC, Lundin JI, Heath JD, Nester EW. Mutational analysis of the input domain of the VirA protein of Agrobacterium tumefaciens. J Bacteriol. 1996 Feb;178(4):961–970. [PMC free article] [PubMed]
  • Finan TM, Kunkel B, De Vos GF, Signer ER. Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J Bacteriol. 1986 Jul;167(1):66–72. [PMC free article] [PubMed]
  • Gish W, States DJ. Identification of protein coding regions by database similarity search. Nat Genet. 1993 Mar;3(3):266–272. [PubMed]
  • Greenwood JA, Cornish A, Jones CW. Binding-protein-dependent lactose transport in Agrobacterium radiobacter. J Bacteriol. 1990 Apr;172(4):1703–1710. [PMC free article] [PubMed]
  • Hayman GT, Beck von Bodman S, Kim H, Jiang P, Farrand SK. Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport. J Bacteriol. 1993 Sep;175(17):5575–5584. [PMC free article] [PubMed]
  • Henikoff S, Haughn GW, Calvo JM, Wallace JC. A large family of bacterial activator proteins. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6602–6606. [PMC free article] [PubMed]
  • Henikoff S, Henikoff JG. Protein family classification based on searching a database of blocks. Genomics. 1994 Jan 1;19(1):97–107. [PubMed]
  • Higgins CF. The role of ATP in binding-protein-dependent transport systems. Res Microbiol. 1990 Mar-Apr;141(3):353–360. [PubMed]
  • Higgins CF. ABC transporters: from microorganisms to man. Annu Rev Cell Biol. 1992;8:67–113. [PubMed]
  • Higgins CF, Hyde SC, Mimmack MM, Gileadi U, Gill DR, Gallagher MP. Binding protein-dependent transport systems. J Bioenerg Biomembr. 1990 Aug;22(4):571–592. [PubMed]
  • Huang ML, Cangelosi GA, Halperin W, Nester EW. A chromosomal Agrobacterium tumefaciens gene required for effective plant signal transduction. J Bacteriol. 1990 Apr;172(4):1814–1822. [PMC free article] [PubMed]
  • Kaniga K, Delor I, Cornelis GR. A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene. 1991 Dec 20;109(1):137–141. [PubMed]
  • Kreusch D, von Lintig J, Schröder J. Ti plasmid-encoded octopine and nopaline catabolism in Agrobacterium: specificities of the LysR-type regulators OccR and NocR, and protein-induced DNA bending. Mol Gen Genet. 1995 Nov 1;249(1):102–110. [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Leong SA, Ditta GS, Helinski DR. Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti. J Biol Chem. 1982 Aug 10;257(15):8724–8730. [PubMed]
  • Manoil C. Analysis of protein localization by use of gene fusions with complementary properties. J Bacteriol. 1990 Feb;172(2):1035–1042. [PMC free article] [PubMed]
  • Maupin-Furlow JA, Rosentel JK, Lee JH, Deppenmeier U, Gunsalus RP, Shanmugam KT. Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli. J Bacteriol. 1995 Sep;177(17):4851–4856. [PMC free article] [PubMed]
  • Prentki P, Krisch HM. In vitro insertional mutagenesis with a selectable DNA fragment. Gene. 1984 Sep;29(3):303–313. [PubMed]
  • Shimoda N, Toyoda-Yamamoto A, Aoki S, Machida Y. Genetic evidence for an interaction between the VirA sensor protein and the ChvE sugar-binding protein of Agrobacterium. J Biol Chem. 1993 Dec 15;268(35):26552–26558. [PubMed]
  • Shimoda N, Toyoda-Yamamoto A, Nagamine J, Usami S, Katayama M, Sakagami Y, Machida Y. Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6684–6688. [PMC free article] [PubMed]
  • Shine J, Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. [PubMed]
  • Sofia HJ, Burland V, Daniels DL, Plunkett G, 3rd, Blattner FR. Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res. 1994 Jul 11;22(13):2576–2586. [PMC free article] [PubMed]
  • Stachel SE, An G, Flores C, Nester EW. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 1985 Apr;4(4):891–898. [PMC free article] [PubMed]
  • Stachel SE, Nester EW, Zambryski PC. A plant cell factor induces Agrobacterium tumefaciens vir gene expression. Proc Natl Acad Sci U S A. 1986 Jan;83(2):379–383. [PMC free article] [PubMed]
  • Valdivia RH, Wang L, Winans SC. Characterization of a putative periplasmic transport system for octopine accumulation encoded by Agrobacterium tumefaciens Ti plasmid pTiA6. J Bacteriol. 1991 Oct;173(20):6398–6405. [PMC free article] [PubMed]
  • Williams SG, Greenwood JA, Jones CW. Agrobacterium radiobacter and related organisms take up fructose via a binding-protein-dependent active-transport system. Microbiology. 1995 Oct;141(Pt 10):2601–2610. [PubMed]
  • Williams SG, Greenwood JA, Jones CW. Molecular analysis of the lac operon encoding the binding-protein-dependent lactose transport system and beta-galactosidase in Agrobacterium radiobacter. Mol Microbiol. 1992 Jul;6(13):1755–1768. [PubMed]
  • Winans SC. Two-way chemical signaling in Agrobacterium-plant interactions. Microbiol Rev. 1992 Mar;56(1):12–31. [PMC free article] [PubMed]
  • Winans SC, Ebert PR, Stachel SE, Gordon MP, Nester EW. A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8278–8282. [PMC free article] [PubMed]
  • Young C, Nester EW. Association of the virD2 protein with the 5' end of T strands in Agrobacterium tumefaciens. J Bacteriol. 1988 Aug;170(8):3367–3374. [PMC free article] [PubMed]
  • Zanker H, von Lintig J, Schröder J. Opine transport genes in the octopine (occ) and nopaline (noc) catabolic regions in Ti plasmids of Agrobacterium tumefaciens. J Bacteriol. 1992 Feb;174(3):841–849. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...