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J Bacteriol. Nov 1996; 178(22): 6587–6598.
PMCID: PMC178546

GlbN (cyanoglobin) is a peripheral membrane protein that is restricted to certain Nostoc spp.

Abstract

The glbN gene of Nostoc commune UTEX 584 is juxtaposed to nifU and nifH, and it encodes a 12-kDa monomeric hemoglobin that binds oxygen with high affinity. In N. commune UTEX 584, maximum accumulation of GlbN occurred in both the heterocysts and vegetative cells of nitrogen-fixing cultures when the rate of oxygen evolution was repressed to less than 25 micromol of O2 mg of chlorophyll a(-1) h(-1). Accumulation of GlbN coincided with maximum synthesis of NifH and ferredoxin NADP+ oxidoreductase (PetH or FNR). A total of 41 strains of cyanobacteria, including 40 nitrogen fixers and representing 16 genera within all five sections of the cyanobacteria were screened for the presence of glbN or GlbN. glbN was present in five Nostoc strains in a single copy. Genomic DNAs from 11 other Nostoc and Anabaena strains, including Anabaena sp. strain PCC 7120, provided no hybridization signals with a glbN probe. A constitutively expressed, 18-kDa protein which cross-reacted strongly with GlbN antibodies was detected in four Anabaena and Nostoc strains and in Trichodesmium thiebautii. The nifU-nifH intergenic region of Nostoc sp. strain MUN 8820 was sequenced (1,229 bp) and was approximately 95% identical to the equivalent region in N. commune UTEX 584. Each strand of the DNA from the nifU-nifH intergenic regions of both strains has the potential to fold into secondary structures in which more than 50% of the bases are internally paired. Mobility shift assays confirmed that NtcA (BifA) bound a site in the nifU-glbN intergenic region of N. commune UTEX 584 approximately 100 bases upstream from the translation initiation site of glbN. This site showed extensive sequence similarity with the promoter region of glnA from Synechococcus sp. strain PCC 7942. In vivo, GlbN had a specific and prominent subcellular location around the periphery of the cytosolic face of the cell membrane, and the protein was found solely in the soluble fraction of cell extracts. Our hypothesis is that GlbN scavenges oxygen for and is a component of a membrane-associated microaerobically induced terminal cytochrome oxidase.

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Selected References

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  • Allen MB, Arnon DI. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. [PMC free article] [PubMed]
  • Angeloni SV, Potts M. Analysis of the sequences within and flanking the cyanoglobin-encoding gene, glbN, of the cyanobacterium Nostoc commune UTEX 584. Gene. 1994 Aug 19;146(1):133–134. [PubMed]
  • Cohen-Kupiec R, Zilberstein A, Gurevitz M. Characterization of cis elements that regulate the expression of glnA in Synechococcus sp. strain PCC 7942. J Bacteriol. 1995 Apr;177(8):2222–2226. [PMC free article] [PubMed]
  • Defrancesco N, Potts M. Cloning of nifHD from Nostoc commune UTEX 584 and of a flanking region homologous to part of the Azotobacter vinelandii nifU gene. J Bacteriol. 1988 Jul;170(7):3297–3300. [PMC free article] [PubMed]
  • Ernst A, Black T, Cai Y, Panoff JM, Tiwari DN, Wolk CP. Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism. J Bacteriol. 1992 Oct;174(19):6025–6032. [PMC free article] [PubMed]
  • Fay P. Oxygen relations of nitrogen fixation in cyanobacteria. Microbiol Rev. 1992 Jun;56(2):340–373. [PMC free article] [PubMed]
  • Gilles-Gonzalez MA, Ditta GS, Helinski DR. A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti. Nature. 1991 Mar 14;350(6314):170–172. [PubMed]
  • Gruber A, Zingales B. Alternative method to remove antibacterial antibodies from antisera used for screening of expression libraries. Biotechniques. 1995 Jul;19(1):28–30. [PubMed]
  • Haselkorn R. Developmentally regulated gene rearrangements in prokaryotes. Annu Rev Genet. 1992;26:113–130. [PubMed]
  • Hill DR, Hladun SL, Scherer S, Potts M. Water stress proteins of Nostoc commune (Cyanobacteria) are secreted with UV-A/B-absorbing pigments and associate with 1,4-beta-D-xylanxylanohydrolase activity. J Biol Chem. 1994 Mar 11;269(10):7726–7734. [PubMed]
  • Jackman DM, Mulligan ME. Characterization of a nitrogen-fixation (nif) gene cluster from Anabaena azollae 1a shows that closely related cyanobacteria have highly variable but structured intergenic regions. Microbiology. 1995 Sep;141(Pt 9):2235–2244. [PubMed]
  • Jaeger JA, Turner DH, Zuker M. Improved predictions of secondary structures for RNA. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7706–7710. [PMC free article] [PubMed]
  • Jaeger JA, Turner DH, Zuker M. Predicting optimal and suboptimal secondary structure for RNA. Methods Enzymol. 1990;183:281–306. [PubMed]
  • Jüngst A, Wakabayashi S, Matsubara H, Zumft WG. The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins. FEBS Lett. 1991 Feb 25;279(2):205–209. [PubMed]
  • Kallio PT, Kim DJ, Tsai PS, Bailey JE. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. Eur J Biochem. 1994 Jan 15;219(1-2):201–208. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Luque I, Flores E, Herrero A. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 1994 Jun 15;13(12):2862–2869. [PMC free article] [PubMed]
  • Lyons EM, Thiel T. Characterization of nifB, nifS, and nifU genes in the cyanobacterium Anabaena variabilis: NifB is required for the vanadium-dependent nitrogenase. J Bacteriol. 1995 Mar;177(6):1570–1575. [PMC free article] [PubMed]
  • Maldener I, Fiedler G, Ernst A, Fernández-Piñas F, Wolk CP. Characterization of devA, a gene required for the maturation of proheterocysts in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1994 Dec;176(24):7543–7549. [PMC free article] [PubMed]
  • Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed]
  • Merril CR, Dunau ML, Goldman D. A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Anal Biochem. 1981 Jan 1;110(1):201–207. [PubMed]
  • Mulligan ME, Haselkorn R. Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120. The nifB-fdxN-nifS-nifU operon. J Biol Chem. 1989 Nov 15;264(32):19200–19207. [PubMed]
  • Murphy ST, Jackman DM, Mulligan ME. Cloning and nucleotide sequence of the gene for dinitrogenase reductase (nifH) from the heterocyst-forming cyanobacterium Anabaena sp. L31. Biochim Biophys Acta. 1993 Jan 23;1171(3):337–340. [PubMed]
  • Olie JJ, Potts M. Purification and Biochemical Analysis of the Cytoplasmic Membrane from the Desiccation-Tolerant Cyanobacterium Nostoc commune UTEX 584. Appl Environ Microbiol. 1986 Oct;52(4):706–710. [PMC free article] [PubMed]
  • Potts M, Angeloni SV, Ebel RE, Bassam D. Myoglobin in a cyanobacterium. Science. 1992 Jun 19;256(5064):1690–1691. [PubMed]
  • Prufert-Bebout L, Paerl HW, Lassen C. Growth, nitrogen fixation, and spectral attenuation in cultivated trichodesmium species. Appl Environ Microbiol. 1993 May;59(5):1367–1375. [PMC free article] [PubMed]
  • Scherer S, Kerfin W, Böger P. Increase of nitrogenase activity in the blue-green alga Nostoc muscorum (Cyanobacterium). J Bacteriol. 1980 Dec;144(3):1017–1023. [PMC free article] [PubMed]
  • Scherer S, Potts M. Novel water stress protein from a desiccation-tolerant cyanobacterium. Purification and partial characterization. J Biol Chem. 1989 Jul 25;264(21):12546–12553. [PubMed]
  • Schrautemeier B, Neveling U, Schmitz S. Distinct and differently regulated Mo-dependent nitrogen-fixing systems evolved for heterocysts and vegetative cells of Anabaena variabilis ATCC 29413: characterization of the fdxH1/2 gene regions as part of the nif1/2 gene clusters. Mol Microbiol. 1995 Oct;18(2):357–369. [PubMed]
  • Smith RL, Van Baalen C, Tabita FR. Alteration of the Fe protein of nitrogenase by oxygen in the cyanobacterium Anabaena sp. strain CA. J Bacteriol. 1987 Jun;169(6):2537–2542. [PMC free article] [PubMed]
  • Stewart WD, Fitzgerald GP, Burris RH. Acetylene reduction by nitrogen-fixing blue-green algae. Arch Mikrobiol. 1968;62(4):336–348. [PubMed]
  • Thiel T, Lyons EM, Erker JC, Ernst A. A second nitrogenase in vegetative cells of a heterocyst-forming cyanobacterium. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9358–9362. [PMC free article] [PubMed]
  • Thorsteinsson MV, Bevan DR, Ebel RE, Weber RE, Potts M. Spectroscopical and functional characterization of the hemoglobin of Nostoc commune (UTEX 584 (Cyanobacterial). Biochim Biophys Acta. 1996 Jan 4;1292(1):133–139. [PubMed]
  • Vasudevan SG, Armarego WL, Shaw DC, Lilley PE, Dixon NE, Poole RK. Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12. Mol Gen Genet. 1991 Apr;226(1-2):49–58. [PubMed]
  • Wei TF, Ramasubramanian TS, Golden JW. Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J Bacteriol. 1994 Aug;176(15):4473–4482. [PMC free article] [PubMed]
  • Wolk CP. Genetic analysis of cyanobacterial development. Curr Opin Genet Dev. 1991 Oct;1(3):336–341. [PubMed]
  • Xie WQ, Potts M. Gene cluster rpoBC1C2 in cyanobacteria does not constitute an operon. Arch Biochem Biophys. 1991 Jan;284(1):22–25. [PubMed]
  • Zuker M. On finding all suboptimal foldings of an RNA molecule. Science. 1989 Apr 7;244(4900):48–52. [PubMed]

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