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J Bacteriol. Oct 1996; 178(20): 6087–6090.
PMCID: PMC178474

Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone.

Abstract

The nucleotide sequence of comC, the gene encoding the 17-residue competence-stimulating peptide (CSP) of Streptococcus pneumoniae (L. S. Havarstein, G. Coomaraswamy, and D. A. Morrison, Proc. Natl. Acad. Sci. USA 92:11140-11144, 1995) was determined with 42 encapsulated strains of different serotypes. A new allele, comC2, was found in 13 strains, including the type 3 Avery strain, A66, while all others carried a gene (now termed comC1) identical to that originally described for strain Rx1. The predicted mature product of comC2 is also a heptadecapeptide but differs from that of comC1 at eight residues. Both CSP-1 and CSP-2 synthetic peptides were used to induce competence in the 42 strains; 48% of the strains became competent after the addition of the synthetic peptide, whereas none were transformable without the added peptides.

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Selected References

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  • AUSTRIAN R, BERNHEIMER HP. Simultaneous production of two capsular polysaccharides by pneumococcus. I. Properties of a pneumococcus manifesting binary capsulation. J Exp Med. 1959 Oct 1;110:571–584. [PMC free article] [PubMed]
  • BRACCO RM, KRAUSS MR, ROE AS, MACLEOD CM. Transformation reactions between Pneumococcus and three strains of Streptococci. J Exp Med. 1957 Aug 1;106(2):247–259. [PMC free article] [PubMed]
  • Coffey TJ, Dowson CG, Daniels M, Zhou J, Martin C, Spratt BG, Musser JM. Horizontal transfer of multiple penicillin-binding protein genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. Mol Microbiol. 1991 Sep;5(9):2255–2260. [PubMed]
  • Dowson CG, Coffey TJ, Kell C, Whiley RA. Evolution of penicillin resistance in Streptococcus pneumoniae; the role of Streptococcus mitis in the formation of a low affinity PBP2B in S. pneumoniae. Mol Microbiol. 1993 Aug;9(3):635–643. [PubMed]
  • Gaustad P. Genetic transformation in Streptococcus sanguis. Competence factor and competence factor inactivator. Acta Pathol Microbiol Scand B. 1981 Apr;89(2):67–73. [PubMed]
  • Gaustad P. Genetic transformation in Streptococcus sanguis. Effects on genetic transformation by culture filtrates of Streptococcus sanguis (serogroups H and W) and streptococcus mitis (mitior) with reference to identification. Acta Pathol Microbiol Immunol Scand B. 1985 Aug;93(4):283–287. [PubMed]
  • Guild WR, Shoemaker NB. Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency. J Bacteriol. 1976 Jan;125(1):125–135. [PMC free article] [PubMed]
  • Håvarstein LS, Holo H, Nes IF. The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by gram-positive bacteria. Microbiology. 1994 Sep;140(Pt 9):2383–2389. [PubMed]
  • MACLEOD CM, KRAUSS MR. Transformation reactions with two non-allelic R mutants of the same strain of Pneumococcus type VIII. J Exp Med. 1956 May 1;103(5):623–632. [PMC free article] [PubMed]
  • Marchese A, Debbia EA, Arvigo A, Pesce A, Schito GC. Susceptibility of Streptococcus pneumoniae strains isolated in Italy to penicillin and ten other antibiotics. J Antimicrob Chemother. 1995 Nov;36(5):833–837. [PubMed]
  • Morrison DA, Baker MF. Competence for genetic transformation in pneumococcus depends on synthesis of a small set of proteins. Nature. 1979 Nov 8;282(5735):215–217. [PubMed]
  • Oggioni MR, Dowson CG, Smith JM, Provvedi R, Pozzi G. The tetracycline resistance gene tet(M) exhibits mosaic structure. Plasmid. 1996 May;35(3):156–163. [PubMed]
  • RAVIN AW. Reciprocal capsular transformations of pneumococci. J Bacteriol. 1959 Mar;77(3):296–309. [PMC free article] [PubMed]
  • Shoemaker NB, Guild WR. Destruction of low efficiency markers is a slow process occurring at a heteroduplex stage of transformation. Mol Gen Genet. 1974;128(4):283–290. [PubMed]
  • Tiraby G, Fox MS, Bernheimer H. Marker discrimination in deoxyribonucleic acid-mediated transformation of various Pneumococcus strains. J Bacteriol. 1975 Feb;121(2):608–618. [PMC free article] [PubMed]
  • TOMASZ A, HOTCHKISS RD. REGULATION OF THE TRANSFORMABILITY OF PHEUMOCOCCAL CULTURES BY MACROMOLECULAR CELL PRODUCTS. Proc Natl Acad Sci U S A. 1964 Mar;51:480–487. [PMC free article] [PubMed]
  • Tomasz A, Mosser JL. On the nature of the pneumococcal activator substance. Proc Natl Acad Sci U S A. 1966 Jan;55(1):58–66. [PMC free article] [PubMed]
  • Yother J, McDaniel LS, Briles DE. Transformation of encapsulated Streptococcus pneumoniae. J Bacteriol. 1986 Dec;168(3):1463–1465. [PMC free article] [PubMed]

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