Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Aug 1996; 178(16): 5027–5031.
PMCID: PMC178291

Isolation and characterization of the structural gene for OmpL, a pressure-regulated porin-like protein from the deep-sea bacterium Photobacterium species strain SS9.


Transposon-directed cloning was used to isolate the ompL gene from the deep-sea bacterium Photobacterium species strain SS9. The deduced amino acid sequence of OmpL displays sequence homology to porin proteins from enteric bacteria. Gene fusion and primer extension analyses indicate that ompL is transcriptionally regulated by pressure.

Full Text

The Full Text of this article is available as a PDF (313K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Bartlett D, Wright M, Yayanos AA, Silverman M. Isolation of a gene regulated by hydrostatic pressure in a deep-sea bacterium. Nature. 1989 Nov 30;342(6249):572–574. [PubMed]
  • Bartlett DH. Microbial life at high pressures. Sci Prog. 1992;76(301-302):479–496. [PubMed]
  • Bartlett D, Chi E. Genetic characterization of ompH mutants in the deep-sea bacterium Photobacterium sp. strain SS9. Arch Microbiol. 1994;162(5):323–328. [PubMed]
  • Bartlett DH, Chi E, Wright ME. Sequence of the ompH gene from the deep-sea bacterium Photobacterium SS9. Gene. 1993 Sep 6;131(1):125–128. [PubMed]
  • Bartlett DH, Welch TJ. ompH gene expression is regulated by multiple environmental cues in addition to high pressure in the deep-sea bacterium Photobacterium species strain SS9. J Bacteriol. 1995 Feb;177(4):1008–1016. [PMC free article] [PubMed]
  • Bauer K, Struyvé M, Bosch D, Benz R, Tommassen J. One single lysine residue is responsible for the special interaction between polyphosphate and the outer membrane porin PhoE of Escherichia coli. J Biol Chem. 1989 Oct 5;264(28):16393–16398. [PubMed]
  • Chi E, Bartlett DH. Use of a reporter gene to follow high-pressure signal transduction in the deep-sea bacterium Photobacterium sp. strain SS9. J Bacteriol. 1993 Dec;175(23):7533–7540. [PMC free article] [PubMed]
  • Chou PY, Fasman GD. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. [PubMed]
  • Elkins C, Carbonetti NH, Coímbre AJ, Thomas CE, Sparling PF. Cloning and constitutive expression of structural genes encoding gonococcal porin protein in Escherichia coli and attenuated Salmonella typhimurium vaccine strains. Gene. 1994 Jan 28;138(1-2):43–50. [PubMed]
  • Graf J, Dunlap PV, Ruby EG. Effect of transposon-induced motility mutations on colonization of the host light organ by Vibrio fischeri. J Bacteriol. 1994 Nov;176(22):6986–6991. [PMC free article] [PubMed]
  • Granger BL, Lazarides E. Desmin and vimentin coexist at the periphery of the myofibril Z disc. Cell. 1979 Dec;18(4):1053–1063. [PubMed]
  • Jeanteur D, Lakey JH, Pattus F. The bacterial porin superfamily: sequence alignment and structure prediction. Mol Microbiol. 1991 Sep;5(9):2153–2164. [PubMed]
  • Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. [PubMed]
  • Mizuno T, Chou MY, Inouye M. A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA). Proc Natl Acad Sci U S A. 1984 Apr;81(7):1966–1970. [PMC free article] [PubMed]
  • Nikaido H, Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev. 1985 Mar;49(1):1–32. [PMC free article] [PubMed]
  • Norrander J, Kempe T, Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. [PubMed]
  • Oliver D. Protein secretion in Escherichia coli. Annu Rev Microbiol. 1985;39:615–648. [PubMed]
  • Struyvé M, Moons M, Tommassen J. Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein. J Mol Biol. 1991 Mar 5;218(1):141–148. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Welch TJ, Farewell A, Neidhardt FC, Bartlett DH. Stress response of Escherichia coli to elevated hydrostatic pressure. J Bacteriol. 1993 Nov;175(22):7170–7177. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...