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J Bacteriol. Aug 1996; 178(16): 5017–5019.
PMCID: PMC178288

Expression of the structural gene, laf1, encoding the flagellin of the lateral flagella in Azospirillum brasilense Sp7.


The induction of the lateral flagella of Azospirillum brasilense Sp7 was studied by using a translational fusion between the laf1 promoter and gusA. The fusion was induced when cells were grown on solid media but not when they were grown in broth. The fusion was also induced by incubation of liquid-grown cells with an anti-polar flagellum polyclonal antiserum. Hindrance of polar-flagellum rotation is suggested to be the signal for this induction.

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Selected References

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  • Del Gallo M, Negi M, Neyra CA. Calcofluor- and lectin-binding exocellular polysaccharides of Azospirillum brasilense and Azospirillum lipoferum. J Bacteriol. 1989 Jun;171(6):3504–3510. [PMC free article] [PubMed]
  • Harshey RM. Bees aren't the only ones: swarming in gram-negative bacteria. Mol Microbiol. 1994 Aug;13(3):389–394. [PubMed]
  • Henrichsen J. Bacterial surface translocation: a survey and a classification. Bacteriol Rev. 1972 Dec;36(4):478–503. [PMC free article] [PubMed]
  • Jefferson RA, Kavanagh TA, Bevan MW. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. [PMC free article] [PubMed]
  • McCarter L, Hilmen M, Silverman M. Flagellar dynamometer controls swarmer cell differentiation of V. parahaemolyticus. Cell. 1988 Jul 29;54(3):345–351. [PubMed]
  • Milcamps A, Van Dommelen A, Stigter J, Vanderleyden J, de Bruijn FJ. The Azospirillum brasilense rpoN gene is involved in nitrogen fixation, nitrate assimilation, ammonium uptake, and flagellar biosynthesis. Can J Microbiol. 1996 May;42(5):467–478. [PubMed]
  • Mishkind M, Raikhel NV, Palevitz BA, Keegstra K. Immunocytochemical localization of wheat germ agglutinin in wheat. J Cell Biol. 1982 Mar;92(3):753–764. [PMC free article] [PubMed]
  • Moens S, Michiels K, Keijers V, Van Leuven F, Vanderleyden J. Cloning, sequencing, and phenotypic analysis of laf1, encoding the flagellin of the lateral flagella of Azospirillum brasilense Sp7. J Bacteriol. 1995 Oct;177(19):5419–5426. [PMC free article] [PubMed]
  • Tarrand JJ, Krieg NR, Döbereiner J. A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol. 1978 Aug;24(8):967–980. [PubMed]

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