• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jan 1996; 178(1): 321–324.
PMCID: PMC177660

Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon.

Abstract

The aceBAK operon was partially induced by a multicopy plasmid which carried the promoter region of the gene which encodes its repressor, iclR. Gel shift and DNase I analyses demonstrated that IclR binds to its own promoter. Disruption of iclR increased the expression of an iclR::lacZ operon fusion. Although aceBAK and iclR are both regulated by IclR, aceBAK expression responds to the carbon source, while expression of iclR does not.

Full Text

The Full Text of this article is available as a PDF (350K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Borthwick AC, Holms WH, Nimmo HG. The phosphorylation of Escherichia coli isocitrate dehydrogenase in intact cells. Biochem J. 1984 Sep 15;222(3):797–804. [PMC free article] [PubMed]
  • Chung T, Klumpp DJ, LaPorte DC. Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map. J Bacteriol. 1988 Jan;170(1):386–392. [PMC free article] [PubMed]
  • Chung T, Resnik E, Stueland C, LaPorte DC. Relative expression of the products of glyoxylate bypass operon: contributions of transcription and translation. J Bacteriol. 1993 Jul;175(14):4572–4575. [PMC free article] [PubMed]
  • Cortay JC, Nègre D, Galinier A, Duclos B, Perrière G, Cozzone AJ. Regulation of the acetate operon in Escherichia coli: purification and functional characterization of the IclR repressor. EMBO J. 1991 Mar;10(3):675–679. [PMC free article] [PubMed]
  • Domdey H, Apostol B, Lin RJ, Newman A, Brody E, Abelson J. Lariat structures are in vivo intermediates in yeast pre-mRNA splicing. Cell. 1984 Dec;39(3 Pt 2):611–621. [PubMed]
  • Fouser LA, Friesen JD. Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing. Cell. 1986 Apr 11;45(1):81–93. [PubMed]
  • Freundlich M, Ramani N, Mathew E, Sirko A, Tsui P. The role of integration host factor in gene expression in Escherichia coli. Mol Microbiol. 1992 Sep;6(18):2557–2563. [PubMed]
  • Garnak M, Reeves HC. Phosphorylation of Isocitrate dehydrogenase of Escherichia coli. Science. 1979 Mar 16;203(4385):1111–1112. [PubMed]
  • Kornberg HL. The role and control of the glyoxylate cycle in Escherichia coli. Biochem J. 1966 Apr;99(1):1–11. [PMC free article] [PubMed]
  • Kornberg HL. The regulation of anaplerotic enzymes in E. coli. Bull Soc Chim Biol (Paris) 1967 Dec 18;49(11):1479–1490. [PubMed]
  • KORNBERG HL, MADSEN NB. Synthesis of C4-dicarboxylic acids from acetate by a glyoxylate bypass of the tricarboxylic acid cycle. Biochim Biophys Acta. 1957 Jun;24(3):651–653. [PubMed]
  • LaPorte DC, Koshland DE., Jr A protein with kinase and phosphatase activities involved in regulation of tricarboxylic acid cycle. Nature. 1982 Dec 2;300(5891):458–460. [PubMed]
  • LaPorte DC, Koshland DE., Jr Phosphorylation of isocitrate dehydrogenase as a demonstration of enhanced sensitivity in covalent regulation. Nature. 1983 Sep 22;305(5932):286–290. [PubMed]
  • Laporte DC, Stueland CS, Ikeda TP. Isocitrate dehydrogenase kinase/phosphatase. Biochimie. 1989 Sep-Oct;71(9-10):1051–1057. [PubMed]
  • LaPorte DC, Walsh K, Koshland DE., Jr The branch point effect. Ultrasensitivity and subsensitivity to metabolic control. J Biol Chem. 1984 Nov 25;259(22):14068–14075. [PubMed]
  • Maloy SR, Nunn WD. Genetic regulation of the glyoxylate shunt in Escherichia coli K-12. J Bacteriol. 1982 Jan;149(1):173–180. [PMC free article] [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Nègre D, Cortay JC, Galinier A, Sauve P, Cozzone AJ. Specific interactions between the IclR repressor of the acetate operon of Escherichia coli and its operator. J Mol Biol. 1992 Nov 5;228(1):23–29. [PubMed]
  • Neidhardt FC, Bloch PL, Smith DF. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. [PMC free article] [PubMed]
  • Parker B, Marinus MG. A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. Gene. 1988 Dec 20;73(2):531–535. [PubMed]
  • Resnik E, LaPorte DC. Introduction of single-copy sequences into the chromosome of Escherichia coli: application to gene and operon fusions. Gene. 1991 Oct 30;107(1):19–25. [PubMed]
  • Sanger F, Coulson AR. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 1975 May 25;94(3):441–448. [PubMed]
  • Shelness GS, Williams DL. Secondary structure analysis of apolipoprotein II mRNA using enzymatic probes and reverse transcriptase. Evaluation of primer extension for high resolution structure mapping of mRNA. J Biol Chem. 1985 Jul 15;260(14):8637–8646. [PubMed]
  • Shih HM, Towle HC. Definition of the carbohydrate response element of the rat S14 gene. Evidence for a common factor required for carbohydrate regulation of hepatic genes. J Biol Chem. 1992 Jul 5;267(19):13222–13228. [PubMed]
  • Summers WC. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate. Anal Biochem. 1970 Feb;33(2):459–463. [PubMed]
  • Sunnarborg A, Klumpp D, Chung T, LaPorte DC. Regulation of the glyoxylate bypass operon: cloning and characterization of iclR. J Bacteriol. 1990 May;172(5):2642–2649. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...