• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Dec 1995; 177(23): 6791–6797.
PMCID: PMC177544

Analysis of cell size and DNA content in exponentially growing and stationary-phase batch cultures of Escherichia coli.

Abstract

Escherichia coli strains were grown in batch cultures in different media, and cell size and DNA content were analyzed by flow cytometry. Steady-state growth required large dilutions and incubation for many generations at low cell concentrations. In rich media, both cell size and DNA content started to decrease at low cell concentrations, long before the cultures left the exponential growth phase. Stationary-phase cultures contained cells with several chromosomes, even after many days, and stationary-phase populations exclusively composed of cells with a single chromosome were never observed, regardless of growth medium. The cells usually contained only one nucleoid, as visualized by phase and fluorescence microscopy. The results have implications for the use of batch cultures to study steady-state and balanced growth and to determine mutation and recombination frequencies in stationary phase.

Full Text

The Full Text of this article is available as a PDF (308K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Akerlund T, Bernander R, Nordström K. Cell division in Escherichia coli minB mutants. Mol Microbiol. 1992 Aug;6(15):2073–2083. [PubMed]
  • Boye E, Løbner-Olesen A. Bacterial growth control studied by flow cytometry. Res Microbiol. 1991 Feb-Apr;142(2-3):131–135. [PubMed]
  • Boye E, Steen HB, Skarstad K. Flow cytometry of bacteria: a promising tool in experimental and clinical microbiology. J Gen Microbiol. 1983 Apr;129(4):973–980. [PubMed]
  • CAMPBELL A. Synchronization of cell division. Bacteriol Rev. 1957 Dec;21(4):263–272. [PMC free article] [PubMed]
  • de Boer PA, Crossley RE, Rothfield LI. A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli. Cell. 1989 Feb 24;56(4):641–649. [PubMed]
  • Fishov I, Zaritsky A, Grover NB. On microbial states of growth. Mol Microbiol. 1995 Mar;15(5):789–794. [PubMed]
  • Foster PL. Adaptive mutation: the uses of adversity. Annu Rev Microbiol. 1993;47:467–504. [PMC free article] [PubMed]
  • Foster PL, Cairns J. Mechanisms of directed mutation. Genetics. 1992 Aug;131(4):783–789. [PMC free article] [PubMed]
  • Grinsted J, Saunders JR, Ingram LC, Sykes RB, Richmond MH. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. [PMC free article] [PubMed]
  • Guyer MS, Reed RR, Steitz JA, Low KB. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):135–140. [PubMed]
  • Helmstetter CE, Pierucci O. DNA synthesis during the division cycle of three substrains of Escherichia coli B/r. J Mol Biol. 1976 Apr 15;102(3):477–486. [PubMed]
  • Lange R, Hengge-Aronis R. Growth phase-regulated expression of bolA and morphology of stationary-phase Escherichia coli cells are controlled by the novel sigma factor sigma S. J Bacteriol. 1991 Jul;173(14):4474–4481. [PMC free article] [PubMed]
  • Painter PR, Marr AG. Mathematics of microbial populations. Annu Rev Microbiol. 1968;22:519–548. [PubMed]
  • Skarstad K, Steen HB, Boye E. Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry. J Bacteriol. 1983 May;154(2):656–662. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...