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J Bacteriol. Nov 1995; 177(22): 6440–6448.
PMCID: PMC177493

The hglK gene is required for localization of heterocyst-specific glycolipids in the cyanobacterium Anabaena sp. strain PCC 7120.

Abstract

Mutant strain 543 of the cyanobacterium Anabaena sp. strain PCC 7120 was originally isolated as a Fox- mutant following chemical mutagenesis. Ultrastructural analysis shows that in nitrogen-replete media the vegetative cells of the mutant are more cylindrical and have thicker septa than those of the wild type, while in nitrogen-free media the mutant heterocysts lack the normal glycolipid layer external to the cell wall. Although this layer is absent, strain 543 heterocysts nevertheless contain heterocyst-specific glycolipids, as determined by thin-layer chromatography. The mutation in strain 543 is in a gene we have named hglK, encoding a protein of 727 amino acids. The wild-type HglK protein appears to contain four membrane-spanning regions followed by 36 repeats of a degenerate pentapeptide sequence, AXLXX. The mutation in strain 543 introduces a termination codon immediately upstream of the pentapeptide repeat region. A mutant constructed by insertion of an antibiotic resistance cassette near the beginning of the hglK gene has the same phenotype as strain 543. We propose that hglK encodes a protein necessary for the localization of heterocyst glycolipids and that this function requires the pentapeptide repeats of the HglK protein.

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Selected References

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  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Black TA, Wolk CP. Analysis of a Het- mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J Bacteriol. 1994 Apr;176(8):2282–2292. [PMC free article] [PubMed]
  • Buikema WJ, Haselkorn R. Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. Genes Dev. 1991 Feb;5(2):321–330. [PubMed]
  • Buikema WJ, Haselkorn R. Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1991 Mar;173(6):1879–1885. [PMC free article] [PubMed]
  • Cai YP, Wolk CP. Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. J Bacteriol. 1990 Jun;172(6):3138–3145. [PMC free article] [PubMed]
  • Cardemil L, Wolk CP. The polysaccharides from heterocyst and spore envelopes of a blue-green alga. Methylation analysis and structure of the backbones. J Biol Chem. 1976 May 25;251(10):2967–2975. [PubMed]
  • Cardemil L, Wolk CP. The polysaccharides from heterocyst and spore envelopes of a blue-green alga. Structure of the basic repeating unit. J Biol Chem. 1979 Feb 10;254(3):736–741. [PubMed]
  • Elhai J, Wolk CP. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol. 1988;167:747–754. [PubMed]
  • Engelman DM, Steitz TA, Goldman A. Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu Rev Biophys Biophys Chem. 1986;15:321–353. [PubMed]
  • Ernst A, Black T, Cai Y, Panoff JM, Tiwari DN, Wolk CP. Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism. J Bacteriol. 1992 Oct;174(19):6025–6032. [PMC free article] [PubMed]
  • Fay P. Oxygen relations of nitrogen fixation in cyanobacteria. Microbiol Rev. 1992 Jun;56(2):340–373. [PMC free article] [PubMed]
  • Fernández-Piñas F, Leganés F, Wolk CP. A third genetic locus required for the formation of heterocysts in Anabaena sp. strain PCC 7120. J Bacteriol. 1994 Sep;176(17):5277–5283. [PMC free article] [PubMed]
  • Fleming H, Haselkorn R. The program of protein synthesis during heterocyst differentiation in nitrogen-fixing blue-green algae. Cell. 1974 Oct;3(2):169–170. [PubMed]
  • Fuchs R. MacPattern: protein pattern searching on the Apple Macintosh. Comput Appl Biosci. 1991 Jan;7(1):105–106. [PubMed]
  • Giovannoni SJ, Turner S, Olsen GJ, Barns S, Lane DJ, Pace NR. Evolutionary relationships among cyanobacteria and green chloroplasts. J Bacteriol. 1988 Aug;170(8):3584–3592. [PMC free article] [PubMed]
  • Golden JW, Wiest DR. Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science. 1988 Dec 9;242(4884):1421–1423. [PubMed]
  • Jaeger JA, Turner DH, Zuker M. Predicting optimal and suboptimal secondary structure for RNA. Methods Enzymol. 1990;183:281–306. [PubMed]
  • Jürgens UJ, Weckesser J. The fine structure and chemical composition of the cell wall and sheath layers of cyanobacteria. Ann Inst Pasteur Microbiol. 1985 Jan-Feb;136A(1):41–44. [PubMed]
  • Jürgens UJ, Weckesser J. Polysaccharide covalently linked to the peptidoglycan of the cyanobacterium Synechocystis sp. strain PCC6714. J Bacteriol. 1986 Nov;168(2):568–573. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Lambein F, Wolk CP. Structural studies on the glycolipids from the envelope of the heterocyst of Anabaena cylindrica. Biochemistry. 1973 Feb 27;12(5):791–798. [PubMed]
  • Liang J, Scappino L, Haselkorn R. The patA gene product, which contains a region similar to CheY of Escherichia coli, controls heterocyst pattern formation in the cyanobacterium Anabaena 7120. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5655–5659. [PMC free article] [PubMed]
  • Liang J, Scappino L, Haselkorn R. The patB gene product, required for growth of the cyanobacterium Anabaena sp. strain PCC 7120 under nitrogen-limiting conditions, contains ferredoxin and helix-turn-helix domains. J Bacteriol. 1993 Mar;175(6):1697–1704. [PMC free article] [PubMed]
  • Lynn ME, Bantle JA, Ownby JD. Estimation of gene expression in heterocysts of Anabaena variabilis by using DNA-RNA hybridization. J Bacteriol. 1986 Sep;167(3):940–946. [PMC free article] [PubMed]
  • Marck C. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Res. 1988 Mar 11;16(5):1829–1836. [PMC free article] [PubMed]
  • Pearson WR, Lipman DJ. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Smith RF, Smith TF. Automatic generation of primary sequence patterns from sets of related protein sequences. Proc Natl Acad Sci U S A. 1990 Jan;87(1):118–122. [PMC free article] [PubMed]
  • von Heijne G. Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J Mol Biol. 1992 May 20;225(2):487–494. [PubMed]
  • Wilcox M, Mitchison GJ, Smith RJ. Pattern formation in the blue-green alga, Anabaena. I. Basic mechanisms. J Cell Sci. 1973 May;12(3):707–723. [PubMed]
  • Wilcox M, Mitchison GJ, Smith RJ. Pattern formation in the blue-green alga Anabaena. II. Controlled proheterocyst regression. J Cell Sci. 1973 Nov;13(3):637–649. [PubMed]
  • Zuker M. On finding all suboptimal foldings of an RNA molecule. Science. 1989 Apr 7;244(4900):48–52. [PubMed]

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