Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 1995 Oct; 177(20): 5991–5993.
PMCID: PMC177429

Functional analysis of sequences adjacent to dapE of Corynebacterium glutamicum reveals the presence of aroP, which encodes the aromatic amino acid transporter.


An initially nonclonable DNA locus close to a gene of L-lysine biosynthesis in Corynebacterium glutamicum was analyzed in detail. Its stepwise cloning and its functional identification by monitoring the amino acid uptakes of defined mutants, together with mechanistic studies, identified the corresponding structure as aroP, the general aromatic amino acid uptake system.

Full Text

The Full Text of this article is available as a PDF (217K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Chye ML, Guest JR, Pittard J. Cloning of the aroP gene and identification of its product in Escherichia coli K-12. J Bacteriol. 1986 Aug;167(2):749–753. [PMC free article] [PubMed]
  • Daniels DL, Plunkett G, 3rd, Burland V, Blattner FR. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science. 1992 Aug 7;257(5071):771–778. [PubMed]
  • Eikmanns BJ, Eggeling L, Sahm H. Molecular aspects of lysine, threonine, and isoleucine biosynthesis in Corynebacterium glutamicum. Antonie Van Leeuwenhoek. 1993;64(2):145–163. [PubMed]
  • Honoré N, Cole ST. Nucleotide sequence of the aroP gene encoding the general aromatic amino acid transport protein of Escherichia coli K-12: homology with yeast transport proteins. Nucleic Acids Res. 1990 Feb 11;18(3):653–653. [PMC free article] [PubMed]
  • Keilhauer C, Eggeling L, Sahm H. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J Bacteriol. 1993 Sep;175(17):5595–5603. [PMC free article] [PubMed]
  • Krämer R, Lambert C. Uptake of glutamate in Corynebacterium glutamicum. 2. Evidence for a primary active transport system. Eur J Biochem. 1990 Dec 27;194(3):937–944. [PubMed]
  • Kronemeyer W, Peekhaus N, Krämer R, Sahm H, Eggeling L. Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum. J Bacteriol. 1995 Mar;177(5):1152–1158. [PMC free article] [PubMed]
  • Reizer J, Finley K, Kakuda D, MacLeod CL, Reizer A, Saier MH., Jr Mammalian integral membrane receptors are homologous to facilitators and antiporters of yeast, fungi, and eubacteria. Protein Sci. 1993 Jan;2(1):20–30. [PMC free article] [PubMed]
  • Schrumpf B, Schwarzer A, Kalinowski J, Pühler A, Eggeling L, Sahm H. A functionally split pathway for lysine synthesis in Corynebacterium glutamicium. J Bacteriol. 1991 Jul;173(14):4510–4516. [PMC free article] [PubMed]
  • Wehrmann A, Eggeling L, Sahm H. Analysis of different DNA fragments of Corynebacterium glutamicum complementing dapE of Escherichia coli. Microbiology. 1994 Dec;140(Pt 12):3349–3356. [PubMed]
  • Vrljic M, Kronemeyer W, Sahm H, Eggeling L. Unbalance of L-lysine flux in Corynebacterium glutamicum and its use for the isolation of excretion-defective mutants. J Bacteriol. 1995 Jul;177(14):4021–4027. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • Gene
    Gene links
  • MedGen
    Related information in MedGen
  • Nucleotide
    Published Nucleotide sequences
  • Protein
    Published protein sequences
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...