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J Bacteriol. Jun 1995; 177(12): 3613–3615.
PMCID: PMC177073

Ferrochelatase activity and protoporphyrin IX utilization in Haemophilus influenzae.

Abstract

Previous research showed that the heme-requiring human pathogen Haemophilus influenzae lacks the first six of the seven enzymes required for heme synthesis, starting with the precursor, 5-amino levulinic acid. In this study, I demonstrated either directly or by reasonable inference that all 57 strains of H. influenzae examined, including 2 unable to grow on protoporphyrin IX, possess ferrochelatase, which catalyzes heme formation by insertion of Fe2+ into the protoporphyrin IX nucleus and which is the last enzyme in the heme synthetic pathway. Further, I showed that this enzyme can also function in the reverse direction, releasing Fe2+ from heme.

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Selected References

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  • Anderson P, Johnston RB, Jr, Smith DH. Human serum activities against Hemophilus influenzae, type b. J Clin Invest. 1972 Jan;51(1):31–38. [PMC free article] [PubMed]
  • Camadro JM, Ibraham NG, Levere RD. Kinetic studies of human liver ferrochelatase. Role of endogenous metals. J Biol Chem. 1984 May 10;259(9):5678–5682. [PubMed]
  • Dailey HA. Purification and characterization of bacterial ferrochelatase. Methods Enzymol. 1986;123:408–415. [PubMed]
  • Evans NM, Smith DD, Wicken AJ. Haemin and nicotinamide adenine dinucleotide requirements of Haemophilus influenzae and Haemophilus parainfluenzae. J Med Microbiol. 1974 Aug;7(3):359–365. [PubMed]
  • Hastka J, Lasserre JJ, Schwarzbeck A, Strauch M, Hehlmann R. Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry. Clin Chem. 1992 Nov;38(11):2184–2189. [PubMed]
  • Herrington DA, Sparling PF. Haemophilus influenzae can use human transferrin as a sole source for required iron. Infect Immun. 1985 Apr;48(1):248–251. [PMC free article] [PubMed]
  • Loeb MR, Smith DH. Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications. Infect Immun. 1980 Dec;30(3):709–717. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Musser JM, Barenkamp SJ, Granoff DM, Selander RK. Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infect Immun. 1986 Apr;52(1):183–191. [PMC free article] [PubMed]
  • Musser JM, Granoff DM, Pattison PE, Selander RK. A population genetic framework for the study of invasive diseases caused by serotype b strains of Haemophilus influenzae. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5078–5082. [PMC free article] [PubMed]
  • Pidcock KA, Wooten JA, Daley BA, Stull TL. Iron acquisition by Haemophilus influenzae. Infect Immun. 1988 Apr;56(4):721–725. [PMC free article] [PubMed]
  • Schwartz S, Stephenson B, Sarkar D, Freyholtz H, Ruth G. Quantitative assay of erythrocyte "free" and zinc-protoporphyrin: clinical and genetic studies. Int J Biochem. 1980;12(5-6):1053–1057. [PubMed]
  • WHITE DC, GRANICK S. HEMIN BIOSYNTHESIS IN HAEMOPHILUS. J Bacteriol. 1963 Apr;85:842–850. [PMC free article] [PubMed]

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