• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. May 1995; 177(9): 2572–2575.
PMCID: PMC176921

Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.

Abstract

The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.

Full Text

The Full Text of this article is available as a PDF (192K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abbott J, Beckett D. Cooperative binding of the Escherichia coli repressor of biotin biosynthesis to the biotin operator sequence. Biochemistry. 1993 Sep 21;32(37):9649–9656. [PubMed]
  • Barker DF, Campbell AM. Use of bio-lac fusion strains to study regulation of biotin biosynthesis in Escherichia coli. J Bacteriol. 1980 Aug;143(2):789–800. [PMC free article] [PubMed]
  • Barker DF, Campbell AM. Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol. 1981 Mar 15;146(4):469–492. [PubMed]
  • Buoncristiani MR, Howard PK, Otsuka AJ. DNA-binding and enzymatic domains of the bifunctional biotin operon repressor (BirA) of Escherichia coli. Gene. 1986;44(2-3):255–261. [PubMed]
  • Campbell A, Del Campillo-Campbell A, Chang R. A mutant of Escherichia coli that requires high concentrations of biotin. Proc Natl Acad Sci U S A. 1972 Mar;69(3):676–680. [PMC free article] [PubMed]
  • Cronan JE., Jr The E. coli bio operon: transcriptional repression by an essential protein modification enzyme. Cell. 1989 Aug 11;58(3):427–429. [PubMed]
  • Dombrosky PM, Schmid MB, Young KD. Sequence divergence of the murB and rrfB genes from Escherichia coli and Salmonella typhimurium. Arch Microbiol. 1994;161(6):501–507. [PubMed]
  • Dubnau D, Davidoff-Abelson R. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. J Mol Biol. 1971 Mar 14;56(2):209–221. [PubMed]
  • Eisenberg MA, Prakash O, Hsiung SC. Purification and properties of the biotin repressor. A bifunctional protein. J Biol Chem. 1982 Dec 25;257(24):15167–15173. [PubMed]
  • Eisenberg MA. Regulation of the biotin operon in E. coli. Ann N Y Acad Sci. 1985;447:335–349. [PubMed]
  • Eisenburg MA, Mee B, Prakash O, Eisenburg MR. Properties of alpha-dehydrobiotin-resistant mutants of Escherichia coli K-12. J Bacteriol. 1975 Apr;122(1):66–72. [PMC free article] [PubMed]
  • Gloeckler R, Ohsawa I, Speck D, Ledoux C, Bernard S, Zinsius M, Villeval D, Kisou T, Kamogawa K, Lemoine Y. Cloning and characterization of the Bacillus sphaericus genes controlling the bioconversion of pimelate into dethiobiotin. Gene. 1990 Mar 1;87(1):63–70. [PubMed]
  • Howard PK, Shaw J, Otsuka AJ. Nucleotide sequence of the birA gene encoding the biotin operon repressor and biotin holoenzyme synthetase functions of Escherichia coli. Gene. 1985;35(3):321–331. [PubMed]
  • Izumi Y, Ogata K. Some aspects of the microbial production of biotin. Adv Appl Microbiol. 1977;22:145–176. [PubMed]
  • Lauer G, Rudd EA, McKay DL, Ally A, Ally D, Backman KC. Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase. J Bacteriol. 1991 Aug;173(16):5047–5053. [PMC free article] [PubMed]
  • Pai CH. Biochemical and genetic characterization of dehydrobiotin resistant mutants of Escherichia coli. Mol Gen Genet. 1974;134(4):345–357. [PubMed]
  • Pai CH. Genetics of biotin biosynthesis in Bacillus subtilis. J Bacteriol. 1975 Jan;121(1):1–8. [PMC free article] [PubMed]
  • Sakurai N, Imai Y, Masuda M, Komatsubara S, Tosa T. Molecular breeding of a biotin-hyperproducing Serratia marcescens strain. Appl Environ Microbiol. 1993 Oct;59(10):3225–3232. [PMC free article] [PubMed]
  • Shiuan D, Campbell A. Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons. Gene. 1988 Jul 30;67(2):203–211. [PubMed]
  • Speck D, Ohsawa I, Gloeckler R, Zinsius M, Bernard S, Ledoux C, Kisou T, Kamogawa K, Lemoine Y. Isolation of Bacillus sphaericus biotin synthesis control mutants: evidence for transcriptional regulation of bio genes. Gene. 1991 Dec 1;108(1):39–45. [PubMed]
  • Suzuki Y, Aoki Y, Ishida Y, Chiba Y, Iwamatsu A, Kishino T, Niikawa N, Matsubara Y, Narisawa K. Isolation and characterization of mutations in the human holocarboxylase synthetase cDNA. Nat Genet. 1994 Oct;8(2):122–128. [PubMed]
  • Wilson KP, Shewchuk LM, Brennan RG, Otsuka AJ, Matthews BW. Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9257–9261. [PMC free article] [PubMed]
  • Xu X, Matsuno-Yagi A, Yagi T. DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans. Biochemistry. 1993 Jan 26;32(3):968–981. [PubMed]
  • Youngman P, Perkins JB, Losick R. Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene. Plasmid. 1984 Jul;12(1):1–9. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links