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J Bacteriol. Jan 1995; 177(2): 439–448.
PMCID: PMC176608

Physical map of the Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052 chromosome.


A combined physical and genetic map of the single, circular, 6.7-Mbp chromosome of the NCIMB 8052 strain of Clostridium beijerinckii (formerly Clostridium acetobutylicum) has been constructed by using a combination of cloned DNA fragments as hybridization probes and a bank of strains harboring insertions of the conjugative transposon Tn1545. The positions of 81 restriction endonuclease cleavage sites and 32 genes have been determined. Eight genes concerned with solventogenic fermentation are found at three different locations. The chromosome contains at least 13 rrn operons, 11 of which have been located on the map. Their transcriptional orientation diverges from the presumed location of the replication origin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allardet-Servent A, Michaux-Charachon S, Jumas-Bilak E, Karayan L, Ramuz M. Presence of one linear and one circular chromosome in the Agrobacterium tumefaciens C58 genome. J Bacteriol. 1993 Dec;175(24):7869–7874. [PMC free article] [PubMed]
  • Amjad M, Castro JM, Sandoval H, Wu JJ, Yang M, Henner DJ, Piggot PJ. An SfiI restriction map of the Bacillus subtilis 168 genome. Gene. 1991 May 15;101(1):15–21. [PubMed]
  • Bancroft I, Wolk CP, Oren EV. Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol. 1989 Nov;171(11):5940–5948. [PMC free article] [PubMed]
  • Baril C, Richaud C, Baranton G, Saint Girons IS. Linear chromosome of Borrelia burgdorferi. Res Microbiol. 1989 Oct;140(8):507–516. [PubMed]
  • Brewer BJ. When polymerases collide: replication and the transcriptional organization of the E. coli chromosome. Cell. 1988 Jun 3;53(5):679–686. [PubMed]
  • Brown DP, Ganova-Raeva L, Green BD, Wilkinson SR, Young M, Youngman P. Characterization of spo0A homologues in diverse Bacillus and Clostridium species identifies a probable DNA-binding domain. Mol Microbiol. 1994 Nov;14(3):411–426. [PubMed]
  • Caillaud F, Carlier C, Courvalin P. Physical analysis of the conjugative shuttle transposon Tn1545. Plasmid. 1987 Jan;17(1):58–60. [PubMed]
  • Caillaud F, Trieu-Cuot P, Carlier C, Courvalin P. Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes. Mol Gen Genet. 1987 May;207(2-3):509–513. [PubMed]
  • Canard B, Cole ST. Genome organization of the anaerobic pathogen Clostridium perfringens. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6676–6680. [PMC free article] [PubMed]
  • Canard B, Saint-Joanis B, Cole ST. Genomic diversity and organization of virulence genes in the pathogenic anaerobe Clostridium perfringens. Mol Microbiol. 1992 Jun;6(11):1421–1429. [PubMed]
  • Carlson CR, Grønstad A, Kolstø AB. Physical maps of the genomes of three Bacillus cereus strains. J Bacteriol. 1992 Jun;174(11):3750–3756. [PMC free article] [PubMed]
  • Carlson CR, Kolstø AB. A complete physical map of a Bacillus thuringiensis chromosome. J Bacteriol. 1993 Feb;175(4):1053–1060. [PMC free article] [PubMed]
  • Cary JW, Petersen DJ, Papoutsakis ET, Bennett GN. Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli. J Bacteriol. 1988 Oct;170(10):4613–4618. [PMC free article] [PubMed]
  • Chen HW, Kuspa A, Keseler IM, Shimkets LJ. Physical map of the Myxococcus xanthus chromosome. J Bacteriol. 1991 Mar;173(6):2109–2115. [PMC free article] [PubMed]
  • Chu G, Vollrath D, Davis RW. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. [PubMed]
  • Cole ST, Saint Girons I. Bacterial genomics. FEMS Microbiol Rev. 1994 Jun;14(2):139–160. [PubMed]
  • Collins MD, Rodrigues UM, Dainty RH, Edwards RA, Roberts TA. Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov. FEMS Microbiol Lett. 1992 Sep 15;75(2-3):235–240. [PubMed]
  • Courvalin P, Carlier C. Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet. 1986 Nov;205(2):291–297. [PubMed]
  • Crespi M, Messens E, Caplan AB, van Montagu M, Desomer J. Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. EMBO J. 1992 Mar;11(3):795–804. [PMC free article] [PubMed]
  • Del Sal G, Manfioletti G, Schneider C. A one-tube plasmid DNA mini-preparation suitable for sequencing. Nucleic Acids Res. 1988 Oct 25;16(20):9878–9878. [PMC free article] [PubMed]
  • Duwat P, Ehrlich SD, Gruss A. A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction. J Bacteriol. 1992 Aug;174(15):5171–5175. [PMC free article] [PubMed]
  • Efstathiou I, Truffaut N. Cloning of Clostridium acetobutylicum genes and their expression in Escherichia coli and Bacillus subtilis. Mol Gen Genet. 1986 Aug;204(2):317–321. [PubMed]
  • Ferdows MS, Barbour AG. Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5969–5973. [PMC free article] [PubMed]
  • Garnier T, Canard B, Cole ST. Cloning, mapping, and molecular characterization of the rRNA operons of Clostridium perfringens. J Bacteriol. 1991 Sep;173(17):5431–5438. [PMC free article] [PubMed]
  • Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. [PubMed]
  • Itaya M, Tanaka T. Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J Mol Biol. 1991 Aug 5;220(3):631–648. [PubMed]
  • Jones DT, Woods DR. Acetone-butanol fermentation revisited. Microbiol Rev. 1986 Dec;50(4):484–524. [PMC free article] [PubMed]
  • Kashket ER, Cao ZY. Isolation of a Degeneration-Resistant Mutant of Clostridium acetobutylicum NCIMB 8052. Appl Environ Microbiol. 1993 Dec;59(12):4198–4202. [PMC free article] [PubMed]
  • Kieser HM, Kieser T, Hopwood DA. A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome. J Bacteriol. 1992 Sep;174(17):5496–5507. [PMC free article] [PubMed]
  • Kolstø AB, Grønstad A, Oppegaard H. Physical map of the Bacillus cereus chromosome. J Bacteriol. 1990 Jul;172(7):3821–3825. [PMC free article] [PubMed]
  • Krawiec S, Riley M. Organization of the bacterial chromosome. Microbiol Rev. 1990 Dec;54(4):502–539. [PMC free article] [PubMed]
  • Kündig C, Hennecke H, Göttfert M. Correlated physical and genetic map of the Bradyrhizobium japonicum 110 genome. J Bacteriol. 1993 Feb;175(3):613–622. [PMC free article] [PubMed]
  • Leblond P, Redenbach M, Cullum J. Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2). J Bacteriol. 1993 Jun;175(11):3422–3429. [PMC free article] [PubMed]
  • Lin YS, Kieser HM, Hopwood DA, Chen CW. The chromosomal DNA of Streptomyces lividans 66 is linear. Mol Microbiol. 1993 Dec;10(5):923–933. [PubMed]
  • Mathieu I, Meyer J, Moulis JM. Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum. Biochem J. 1992 Jul 1;285(Pt 1):255–262. [PMC free article] [PubMed]
  • Mermelstein LD, Welker NE, Bennett GN, Papoutsakis ET. Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Biotechnology (N Y) 1992 Feb;10(2):190–195. [PubMed]
  • Meyer J. Cloning and sequencing of the gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum. Biochim Biophys Acta. 1993 Jul 18;1174(1):108–110. [PubMed]
  • Michaux S, Paillisson J, Carles-Nurit MJ, Bourg G, Allardet-Servent A, Ramuz M. Presence of two independent chromosomes in the Brucella melitensis 16M genome. J Bacteriol. 1993 Feb;175(3):701–705. [PMC free article] [PubMed]
  • Narberhaus F, Bahl H. Cloning, sequencing, and molecular analysis of the groESL operon of Clostridium acetobutylicum. J Bacteriol. 1992 May;174(10):3282–3289. [PMC free article] [PubMed]
  • Narberhaus F, Giebeler K, Bahl H. Molecular characterization of the dnaK gene region of Clostridium acetobutylicum, including grpE, dnaJ, and a new heat shock gene. J Bacteriol. 1992 May;174(10):3290–3299. [PMC free article] [PubMed]
  • Neumann B, Pospiech A, Schairer HU. A physical and genetic map of the Stigmatella aurantiaca DW4/3.1 chromosome. Mol Microbiol. 1993 Dec;10(5):1087–1099. [PubMed]
  • O'Brien RW, Morris JG. Oxygen and the growth and metabolism of Clostridium acetobutylicum. J Gen Microbiol. 1971 Nov;68(3):307–318. [PubMed]
  • Petersen DJ, Bennett GN. Purification of acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824 and cloning of the acetoacetate decarboxylase gene in Escherichia coli. Appl Environ Microbiol. 1990 Nov;56(11):3491–3498. [PMC free article] [PubMed]
  • Petersen DJ, Bennett GN. Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (thiolase; EC gene. Appl Environ Microbiol. 1991 Sep;57(9):2735–2741. [PMC free article] [PubMed]
  • Petersen DJ, Cary JW, Vanderleyden J, Bennett GN. Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824. Gene. 1993 Jan 15;123(1):93–97. [PubMed]
  • Schwartz DC, Cantor CR. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. [PubMed]
  • Smith CL, Cantor CR. Purification, specific fragmentation, and separation of large DNA molecules. Methods Enzymol. 1987;155:449–467. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Williams DR, Young DI, Young M. Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum. J Gen Microbiol. 1990 May;136(5):819–826. [PubMed]
  • Woolley RC, Pennock A, Ashton RJ, Davies A, Young M. Transfer of Tn1545 and Tn916 to Clostridium acetobutylicum. Plasmid. 1989 Sep;22(2):169–174. [PubMed]
  • Young M, Minton NP, Staudenbauer WL. Recent advances in the genetics of the clostridia. FEMS Microbiol Rev. 1989 Dec;5(4):301–325. [PubMed]
  • Youngleson JS, Jones DT, Woods DR. Homology between hydroxybutyryl and hydroxyacyl coenzyme A dehydrogenase enzymes from Clostridium acetobutylicum fermentation and vertebrate fatty acid beta-oxidation pathways. J Bacteriol. 1989 Dec;171(12):6800–6807. [PMC free article] [PubMed]
  • Zuerner RL, Herrmann JL, Saint Girons I. Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity. J Bacteriol. 1993 Sep;175(17):5445–5451. [PMC free article] [PubMed]

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