• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of annrheumdAnnals of the Rheumatic DiseasesCurrent TOCInstructions for authors
Ann Rheum Dis. Jun 2002; 61(6): 534–537.
PMCID: PMC1754106

Histological assessment of the early enthesitis lesion in spondyloarthropathy

Abstract

Methods: Clinically evident acute enthesopathy was confirmed by magnetic resonance imaging and ultrasonography in four cases of plantar fasciitis and one case of patellar tendon enthesitis. Ultrasound guided biopsy of insertional points was carried out with a Jamshedi needle. Control tissue was obtained from two subjects undergoing spinal grafting surgery. Standard histochemistry and immunohistochemistry analysis using the avidin-biotin immunoperoxidase complex method employing markers against CD3, CD8, CD34, and CD68 was used to determine cellular infiltrates at the insertion point.

Results: The enthesis architecture was abnormal in the SpA group, with increased vascularity and cellular infiltration compared with normal subjects. The predominant infiltrating cell at the enthesis fibrocartilage was the macrophage, but there was a paucity of lymphocytes at the insertion point.

Conclusion: These preliminary findings have implications for a better understanding of the pathology in early SpA.

Full Text

The Full Text of this article is available as a PDF (158K).

Figures and Tables

Figure 1
Histology of the plantar fascia employing haematoxylin and eosin (H&E) staining. (A) The sample shows an inflammatory cell infiltrate in the fibrous part of the enthesis with increased vascularity at that site (arrows). The adjacent bone is marked ...
Figure 2
Immunohistochemistry using the ABC method showing the presence of macrophages in the enthesis in fibrous tissue at enthesis (open arrows) (A) and in the entheseal fibrocartilage (B) with prominent CD68 positive staining. There is no CD3 (C) or CD8 (D) ...

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...