• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Jun 1997; 65(6): 2011–2018.
PMCID: PMC175278

Distribution of drb genes coding for Dr binding adhesins among uropathogenic and fecal Escherichia coli isolates and identification of new subtypes.

Abstract

The Dr family of related adherence structures, some fimbriated and others afimbriated, bind to decay-accelerating factor molecules on human cells. Dr is associated with recurring urinary tract infection (UTI), but the distribution of Dr subtypes among uropathogenic Escherichia coli causing UTI among otherwise healthy women has yet to be described. A total of 787 UTI and fecal E. coli isolates from college women were screened for the presence of Dr sequences (drb). Fifteen percent of UTI strains were drb positive, compared to 5% of fecal strains. The adhesin (E gene) subtype of each drb-positive strain was determined by type-specific PCR followed by restriction enzyme analysis. Among 78 drb-positive strains, we found 14 (18%) afaE1, 1 (1.3%) afaE2, 1 (1.3%) afaE3, 9 (12%) draE, 9 (12%) draE-afaE3 hybrid, 1 (1.3%) daaE, 32 (41%) afaE5, 4 (5.1%) F131 E gene-like, and 7 untypeable strains. All untypeable E genes were cloned and sequenced, revealing four additional new classes of E genes, including two similar to the previously identified nonfimbrial E series. While a great range of diversity exists among the E genes, restriction fragment length polymorphism analysis demonstrated that all of these drb operons share a highly conserved gene structure. The most common subtype, afaE5, occurred three times as often among UTI than fecal strains. Over half of the drb-positive strains and 80% of those positive for afaE5 have the same virulence signature (positive for aer, kpsMT, ompT, and fim), suggesting an association of this profile with UTI pathogenesis.

Full Text

The Full Text of this article is available as a PDF (1.0M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ahrens R, Ott M, Ritter A, Hoschützky H, Bühler T, Lottspeich F, Boulnois GJ, Jann K, Hacker J. Genetic analysis of the gene cluster encoding nonfimbrial adhesin I from an Escherichia coli uropathogen. Infect Immun. 1993 Jun;61(6):2505–2512. [PMC free article] [PubMed]
  • Arthur M, Johnson CE, Rubin RH, Arbeit RD, Campanelli C, Kim C, Steinbach S, Agarwal M, Wilkinson R, Goldstein R. Molecular epidemiology of adhesin and hemolysin virulence factors among uropathogenic Escherichia coli. Infect Immun. 1989 Feb;57(2):303–313. [PMC free article] [PubMed]
  • Aubel D, Darfeuille-Michaud A, Martin C, Joly B. Nucleotide sequence of the nfaA gene encoding the antigen 8786 adhesive factor of enterotoxigenic Escherichia coli. FEMS Microbiol Lett. 1992 Nov 1;77(1-3):277–284. [PubMed]
  • Bilge SS, Apostol JM, Jr, Fullner KJ, Moseley SL. Transcriptional organization of the F1845 fimbrial adhesin determinant of Escherichia coli. Mol Microbiol. 1993 Mar;7(6):993–1006. [PubMed]
  • Bilge SS, Clausen CR, Lau W, Moseley SL. Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J Bacteriol. 1989 Aug;171(8):4281–4289. [PMC free article] [PubMed]
  • Blum G, Ott M, Lischewski A, Ritter A, Imrich H, Tschäpe H, Hacker J. Excision of large DNA regions termed pathogenicity islands from tRNA-specific loci in the chromosome of an Escherichia coli wild-type pathogen. Infect Immun. 1994 Feb;62(2):606–614. [PMC free article] [PubMed]
  • Carnoy C, Moseley SL. Mutational analysis of receptor binding mediated by the Dr family of Escherichia coli adhesins. Mol Microbiol. 1997 Jan;23(2):365–379. [PubMed]
  • Daigle F, Harel J, Fairbrother JM, Lebel P. Expression and detection of pap-, sfa-, and afa-encoded fimbrial adhesin systems among uropathogenic Escherichia coli. Can J Microbiol. 1994 Apr;40(4):286–291. [PubMed]
  • De Graaf FK, Mooi FR. The fimbrial adhesins of Escherichia coli. Adv Microb Physiol. 1986;28:65–143. [PubMed]
  • Foxman B. Recurring urinary tract infection: incidence and risk factors. Am J Public Health. 1990 Mar;80(3):331–333. [PMC free article] [PubMed]
  • Foxman B, Zhang L, Palin K, Tallman P, Marrs CF. Bacterial virulence characteristics of Escherichia coli isolates from first-time urinary tract infection. J Infect Dis. 1995 Jun;171(6):1514–1521. [PubMed]
  • Foxman B, Zhang L, Tallman P, Palin K, Rode C, Bloch C, Gillespie B, Marrs CF. Virulence characteristics of Escherichia coli causing first urinary tract infection predict risk of second infection. J Infect Dis. 1995 Dec;172(6):1536–1541. [PubMed]
  • Garcia MI, Gounon P, Courcoux P, Labigne A, Le Bouguenec C. The afimbrial adhesive sheath encoded by the afa-3 gene cluster of pathogenic Escherichia coli is composed of two adhesins. Mol Microbiol. 1996 Feb;19(4):683–693. [PubMed]
  • Garcia MI, Labigne A, Le Bouguenec C. Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster and its translocation via flanking IS1 insertion sequences. J Bacteriol. 1994 Dec;176(24):7601–7613. [PMC free article] [PubMed]
  • Goldhar J, Perry R, Golecki JR, Hoschutzky H, Jann B, Jann K. Nonfimbrial, mannose-resistant adhesins from uropathogenic Escherichia coli O83:K1:H4 and O14:K?:H11. Infect Immun. 1987 Aug;55(8):1837–1842. [PMC free article] [PubMed]
  • Hales BA, Beverley-Clarke H, High NJ, Jann K, Perry R, Goldhar J, Boulnois GJ. Molecular cloning and characterisation of the genes for a non-fimbrial adhesin from Escherichia coli. Microb Pathog. 1988 Jul;5(1):9–17. [PubMed]
  • Hull RA, Gill RE, Hsu P, Minshew BH, Falkow S. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981 Sep;33(3):933–938. [PMC free article] [PubMed]
  • Johnson JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991 Jan;4(1):80–128. [PMC free article] [PubMed]
  • Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. [PMC free article] [PubMed]
  • Krogfelt KA. Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli. Rev Infect Dis. 1991 Jul-Aug;13(4):721–735. [PubMed]
  • Labigne-Roussel A, Falkow S. Distribution and degree of heterogeneity of the afimbrial-adhesin-encoding operon (afa) among uropathogenic Escherichia coli isolates. Infect Immun. 1988 Mar;56(3):640–648. [PMC free article] [PubMed]
  • Labigne-Roussel A, Schmidt MA, Walz W, Falkow S. Genetic organization of the afimbrial adhesin operon and nucleotide sequence from a uropathogenic Escherichia coli gene encoding an afimbrial adhesin. J Bacteriol. 1985 Jun;162(3):1285–1292. [PMC free article] [PubMed]
  • Le Bouguenec C, Garcia MI, Ouin V, Desperrier JM, Gounon P, Labigne A. Characterization of plasmid-borne afa-3 gene clusters encoding afimbrial adhesins expressed by Escherichia coli strains associated with intestinal or urinary tract infections. Infect Immun. 1993 Dec;61(12):5106–5114. [PMC free article] [PubMed]
  • Nowicki B, Hart A, Coyne KE, Lublin DM, Nowicki S. Short consensus repeat-3 domain of recombinant decay-accelerating factor is recognized by Escherichia coli recombinant Dr adhesin in a model of a cell-cell interaction. J Exp Med. 1993 Dec 1;178(6):2115–2121. [PMC free article] [PubMed]
  • Nowicki B, Labigne A, Moseley S, Hull R, Hull S, Moulds J. The Dr hemagglutinin, afimbrial adhesins AFA-I and AFA-III, and F1845 fimbriae of uropathogenic and diarrhea-associated Escherichia coli belong to a family of hemagglutinins with Dr receptor recognition. Infect Immun. 1990 Jan;58(1):279–281. [PMC free article] [PubMed]
  • Nowicki B, Svanborg-Edén C, Hull R, Hull S. Molecular analysis and epidemiology of the Dr hemagglutinin of uropathogenic Escherichia coli. Infect Immun. 1989 Feb;57(2):446–451. [PMC free article] [PubMed]
  • Savarino SJ, Fox P, Deng Y, Nataro JP. Identification and characterization of a gene cluster mediating enteroaggregative Escherichia coli aggregative adherence fimbria I biogenesis. J Bacteriol. 1994 Aug;176(16):4949–4957. [PMC free article] [PubMed]
  • Stapleton A, Moseley S, Stamm WE. Urovirulence determinants in Escherichia coli isolates causing first-episode and recurrent cystitis in women. J Infect Dis. 1991 Apr;163(4):773–779. [PubMed]
  • Swanson TN, Bilge SS, Nowicki B, Moseley SL. Molecular structure of the Dr adhesin: nucleotide sequence and mapping of receptor-binding domain by use of fusion constructs. Infect Immun. 1991 Jan;59(1):261–268. [PMC free article] [PubMed]
  • Swenson DL, Bukanov NO, Berg DE, Welch RA. Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. Infect Immun. 1996 Sep;64(9):3736–3743. [PMC free article] [PubMed]
  • Westerlund B, Kuusela P, Risteli J, Risteli L, Vartio T, Rauvala H, Virkola R, Korhonen TK. The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen-binding protein. Mol Microbiol. 1989 Mar;3(3):329–337. [PubMed]
  • Yamamoto S, Tsukamoto T, Terai A, Kurazono H, Takeda Y, Yoshida O. Distribution of virulence factors in Escherichia coli isolated from urine of cystitis patients. Microbiol Immunol. 1995;39(6):401–404. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...