• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. May 1997; 65(5): 1960–1961.
PMCID: PMC175252

Nutritional requirements for growth of uropathogenic Escherichia coli in human urine.

Abstract

Enrichment with D-cycloserine was used to identify Escherichia coli auxotrophic mutants that exhibited limited growth in human urine. Bacterial synthesis of guanine, arginine, and glutamine was found to be required for optimal growth in urine. Mutants that required leucine, methionine, serine, phenylalanine, or proline also exhibited reduced growth in urine. Several other nutritional mutants, including nicotinamide auxotrophs, which are found frequently among cystitis isolates, exhibited normal growth in urine.

Full Text

The Full Text of this article is available as a PDF (108K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abdelal AT, Griego E, Ingraham JL. Arginine auxotrophic phenotype of mutation in pyrA of Salmonella typhimurium: role of N-acetylornithine in the maturation of mutant carbamylphosphate synthetase. J Bacteriol. 1978 May;134(2):528–536. [PMC free article] [PubMed]
  • Curtiss R, 3rd, Charamella LJ, Berg CM, Harris PE. Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1238–1250. [PMC free article] [PubMed]
  • DAVIS BD, MINGIOLI ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. [PMC free article] [PubMed]
  • Gordon DM, Riley MA. A theoretical and experimental analysis of bacterial growth in the bladder. Mol Microbiol. 1992 Feb;6(4):555–562. [PubMed]
  • Kunin CM, Hua TH, Krishnan C, White LV. Do temperature-sensitive auxotrophs of Escherichia coli have special virulence? J Clin Microbiol. 1993 Jan;31(1):47–49. [PMC free article] [PubMed]
  • Merrick MJ, Edwards RA. Nitrogen control in bacteria. Microbiol Rev. 1995 Dec;59(4):604–622. [PMC free article] [PubMed]
  • Russo TA, Jodush ST, Brown JJ, Johnson JR. Identification of two previously unrecognized genes (guaA and argC) important for uropathogenesis. Mol Microbiol. 1996 Oct;22(2):217–229. [PubMed]
  • Stamey TA, Mihara G. Observations on the growth of urethral and vaginal bacteria in sterile urine. J Urol. 1980 Oct;124(4):461–463. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats: