• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Apr 1997; 65(4): 1546–1549.
PMCID: PMC175168

Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells.

Abstract

Binding of the two identified type 3 fimbrial variants of Klebsiella pneumoniae to human endothelial EA-hy926 and bladder T24 cells was assessed. The recombinant Escherichia coli strain LE392(pFK12), expressing plasmid-encoded type 3 fimbriae of K. pneumoniae, adhered to both cell lines, and the fimbriae purified from the strain bound to both cell lines in a dose-dependent manner. Adhesiveness to both cell lines of chromosomally encoded type 3 fimbriae from K. pneumoniae IApc35 was lower. No binding was detected with type 1 fimbriae of K. pneumoniae. Both type 3 fimbrial variants exhibited a significantly lower affinity for the cell lines than did S fimbriae of meningitis-associated E. coli.

Full Text

The Full Text of this article is available as a PDF (264K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cryz SJ, Jr, Mortimer PM, Mansfield V, Germanier R. Seroepidemiology of Klebsiella bacteremic isolates and implications for vaccine development. J Clin Microbiol. 1986 Apr;23(4):687–690. [PMC free article] [PubMed]
  • Darfeuille-Michaud A, Jallat C, Aubel D, Sirot D, Rich C, Sirot J, Joly B. R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections. Infect Immun. 1992 Jan;60(1):44–55. [PMC free article] [PubMed]
  • Di Martino P, Bertin Y, Girardeau JP, Livrelli V, Joly B, Darfeuille-Michaud A. Molecular characterization and adhesive properties of CF29K, an adhesin of Klebsiella pneumoniae strains involved in nosocomial infections. Infect Immun. 1995 Nov;63(11):4336–4344. [PMC free article] [PubMed]
  • Di Martino P, Livrelli V, Sirot D, Joly B, Darfeuille-Michaud A. A new fimbrial antigen harbored by CAZ-5/SHV-4-producing Klebsiella pneumoniae strains involved in nosocomial infections. Infect Immun. 1996 Jun;64(6):2266–2273. [PMC free article] [PubMed]
  • DUGUID JP. Fimbriae and adhesive properties in Klebsiella strains. J Gen Microbiol. 1959 Aug;21:271–286. [PubMed]
  • Edgell CJ, McDonald CC, Graham JB. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3734–3737. [PMC free article] [PubMed]
  • Favre-Bonte S, Darfeuille-Michaud A, Forestier C. Aggregative adherence of Klebsiella pneumoniae to human intestine-407 cells. Infect Immun. 1995 Apr;63(4):1318–1328. [PMC free article] [PubMed]
  • Gerlach GF, Allen BL, Clegg S. Molecular characterization of the type 3 (MR/K) fimbriae of Klebsiella pneumoniae. J Bacteriol. 1988 Aug;170(8):3547–3553. [PMC free article] [PubMed]
  • Gerlach GF, Clegg S, Allen BL. Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae. J Bacteriol. 1989 Mar;171(3):1262–1270. [PMC free article] [PubMed]
  • Hacker J, Schmidt G, Hughes C, Knapp S, Marget M, Goebel W. Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain. Infect Immun. 1985 Feb;47(2):434–440. [PMC free article] [PubMed]
  • Hornick DB, Allen BL, Horn MA, Clegg S. Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae. J Clin Microbiol. 1991 Sep;29(9):1795–1800. [PMC free article] [PubMed]
  • Hornick DB, Allen BL, Horn MA, Clegg S. Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products. Infect Immun. 1992 Apr;60(4):1577–1588. [PMC free article] [PubMed]
  • Hornick DB, Thommandru J, Smits W, Clegg S. Adherence properties of an mrkD-negative mutant of Klebsiella pneumoniae. Infect Immun. 1995 May;63(5):2026–2032. [PMC free article] [PubMed]
  • Korhonen TK, Nurmiaho EL, Ranta H, Edén CS. New Method for isolation of immunologically pure pili from Escherichia coli. Infect Immun. 1980 Feb;27(2):569–575. [PMC free article] [PubMed]
  • Livrelli V, De Champs C, Di Martino P, Darfeuille-Michaud A, Forestier C, Joly B. Adhesive properties and antibiotic resistance of Klebsiella, Enterobacter, and Serratia clinical isolates involved in nosocomial infections. J Clin Microbiol. 1996 Aug;34(8):1963–1969. [PMC free article] [PubMed]
  • Maayan MC, Ofek I, Medalia O, Aronson M. Population shift in mannose-specific fimbriated phase of Klebsiella pneumoniae during experimental urinary tract infection in mice. Infect Immun. 1985 Sep;49(3):785–789. [PMC free article] [PubMed]
  • Morschhäuser J, Hoschützky H, Jann K, Hacker J. Functional analysis of the sialic acid-binding adhesin SfaS of pathogenic Escherichia coli by site-specific mutagenesis. Infect Immun. 1990 Jul;58(7):2133–2138. [PMC free article] [PubMed]
  • Nowicki B, Truong L, Moulds J, Hull R. Presence of the Dr receptor in normal human tissues and its possible role in the pathogenesis of ascending urinary tract infection. Am J Pathol. 1988 Oct;133(1):1–4. [PMC free article] [PubMed]
  • |ORSKOV I. Nosocomial infections with Klebsiella in lesions of the urinary tract. Acta Pathol Microbiol Scand Suppl. 1952 Jun;93:259–271. [PubMed]
  • Parkkinen J, Korhonen TK, Pere A, Hacker J, Soinila S. Binding sites in the rat brain for Escherichia coli S fimbriae associated with neonatal meningitis. J Clin Invest. 1988 Mar;81(3):860–865. [PMC free article] [PubMed]
  • Parkkinen J, Ristimäki A, Westerlund B. Binding of Escherichia coli S fimbriae to cultured human endothelial cells. Infect Immun. 1989 Jul;57(7):2256–2259. [PMC free article] [PubMed]
  • Podschun R, Heineken P, Sonntag HG. Haemagglutinins and adherence properties to HeLa and intestine 407 cells of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Mar;263(4):585–593. [PubMed]
  • Podschun R, Sievers D, Fischer A, Ullmann U. Serotypes, hemagglutinins, siderophore synthesis, and serum resistance of Klebsiella isolates causing human urinary tract infections. J Infect Dis. 1993 Dec;168(6):1415–1421. [PubMed]
  • Schurtz TA, Hornick DB, Korhonen TK, Clegg S. The type 3 fimbrial adhesin gene (mrkD) of Klebsiella species is not conserved among all fimbriate strains. Infect Immun. 1994 Oct;62(10):4186–4191. [PMC free article] [PubMed]
  • Smith SM, Digori JT, Eng RH. Epidemiology of Klebsiella antibiotic resistance and serotypes. J Clin Microbiol. 1982 Nov;16(5):868–873. [PMC free article] [PubMed]
  • Tarkkanen AM, Allen BL, Westerlund B, Holthöfer H, Kuusela P, Risteli L, Clegg S, Korhonen TK. Type V collagen as the target for type-3 fimbriae, enterobacterial adherence organelles. Mol Microbiol. 1990 Aug;4(8):1353–1361. [PubMed]
  • Tarkkanen AM, Allen BL, Williams PH, Kauppi M, Haahtela K, Siitonen A, Orskov I, Orskov F, Clegg S, Korhonen TK. Fimbriation, capsulation, and iron-scavenging systems of Klebsiella strains associated with human urinary tract infection. Infect Immun. 1992 Mar;60(3):1187–1192. [PMC free article] [PubMed]
  • Virkola R, Westerlund B, Holthöfer H, Parkkinen J, Kekomäki M, Korhonen TK. Binding characteristics of Escherichia coli adhesins in human urinary bladder. Infect Immun. 1988 Oct;56(10):2615–2622. [PMC free article] [PubMed]
  • Westerlund B, Korhonen TK. Bacterial proteins binding to the mammalian extracellular matrix. Mol Microbiol. 1993 Aug;9(4):687–694. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...