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Infect Immun. Apr 1997; 65(4): 1546–1549.
PMCID: PMC175168

Binding of the type 3 fimbriae of Klebsiella pneumoniae to human endothelial and urinary bladder cells.


Binding of the two identified type 3 fimbrial variants of Klebsiella pneumoniae to human endothelial EA-hy926 and bladder T24 cells was assessed. The recombinant Escherichia coli strain LE392(pFK12), expressing plasmid-encoded type 3 fimbriae of K. pneumoniae, adhered to both cell lines, and the fimbriae purified from the strain bound to both cell lines in a dose-dependent manner. Adhesiveness to both cell lines of chromosomally encoded type 3 fimbriae from K. pneumoniae IApc35 was lower. No binding was detected with type 1 fimbriae of K. pneumoniae. Both type 3 fimbrial variants exhibited a significantly lower affinity for the cell lines than did S fimbriae of meningitis-associated E. coli.

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  • Cryz SJ, Jr, Mortimer PM, Mansfield V, Germanier R. Seroepidemiology of Klebsiella bacteremic isolates and implications for vaccine development. J Clin Microbiol. 1986 Apr;23(4):687–690. [PMC free article] [PubMed]
  • Darfeuille-Michaud A, Jallat C, Aubel D, Sirot D, Rich C, Sirot J, Joly B. R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections. Infect Immun. 1992 Jan;60(1):44–55. [PMC free article] [PubMed]
  • Di Martino P, Bertin Y, Girardeau JP, Livrelli V, Joly B, Darfeuille-Michaud A. Molecular characterization and adhesive properties of CF29K, an adhesin of Klebsiella pneumoniae strains involved in nosocomial infections. Infect Immun. 1995 Nov;63(11):4336–4344. [PMC free article] [PubMed]
  • Di Martino P, Livrelli V, Sirot D, Joly B, Darfeuille-Michaud A. A new fimbrial antigen harbored by CAZ-5/SHV-4-producing Klebsiella pneumoniae strains involved in nosocomial infections. Infect Immun. 1996 Jun;64(6):2266–2273. [PMC free article] [PubMed]
  • DUGUID JP. Fimbriae and adhesive properties in Klebsiella strains. J Gen Microbiol. 1959 Aug;21:271–286. [PubMed]
  • Edgell CJ, McDonald CC, Graham JB. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3734–3737. [PMC free article] [PubMed]
  • Favre-Bonte S, Darfeuille-Michaud A, Forestier C. Aggregative adherence of Klebsiella pneumoniae to human intestine-407 cells. Infect Immun. 1995 Apr;63(4):1318–1328. [PMC free article] [PubMed]
  • Gerlach GF, Allen BL, Clegg S. Molecular characterization of the type 3 (MR/K) fimbriae of Klebsiella pneumoniae. J Bacteriol. 1988 Aug;170(8):3547–3553. [PMC free article] [PubMed]
  • Gerlach GF, Clegg S, Allen BL. Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae. J Bacteriol. 1989 Mar;171(3):1262–1270. [PMC free article] [PubMed]
  • Hacker J, Schmidt G, Hughes C, Knapp S, Marget M, Goebel W. Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain. Infect Immun. 1985 Feb;47(2):434–440. [PMC free article] [PubMed]
  • Hornick DB, Allen BL, Horn MA, Clegg S. Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae. J Clin Microbiol. 1991 Sep;29(9):1795–1800. [PMC free article] [PubMed]
  • Hornick DB, Allen BL, Horn MA, Clegg S. Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products. Infect Immun. 1992 Apr;60(4):1577–1588. [PMC free article] [PubMed]
  • Hornick DB, Thommandru J, Smits W, Clegg S. Adherence properties of an mrkD-negative mutant of Klebsiella pneumoniae. Infect Immun. 1995 May;63(5):2026–2032. [PMC free article] [PubMed]
  • Korhonen TK, Nurmiaho EL, Ranta H, Edén CS. New Method for isolation of immunologically pure pili from Escherichia coli. Infect Immun. 1980 Feb;27(2):569–575. [PMC free article] [PubMed]
  • Livrelli V, De Champs C, Di Martino P, Darfeuille-Michaud A, Forestier C, Joly B. Adhesive properties and antibiotic resistance of Klebsiella, Enterobacter, and Serratia clinical isolates involved in nosocomial infections. J Clin Microbiol. 1996 Aug;34(8):1963–1969. [PMC free article] [PubMed]
  • Maayan MC, Ofek I, Medalia O, Aronson M. Population shift in mannose-specific fimbriated phase of Klebsiella pneumoniae during experimental urinary tract infection in mice. Infect Immun. 1985 Sep;49(3):785–789. [PMC free article] [PubMed]
  • Morschhäuser J, Hoschützky H, Jann K, Hacker J. Functional analysis of the sialic acid-binding adhesin SfaS of pathogenic Escherichia coli by site-specific mutagenesis. Infect Immun. 1990 Jul;58(7):2133–2138. [PMC free article] [PubMed]
  • Nowicki B, Truong L, Moulds J, Hull R. Presence of the Dr receptor in normal human tissues and its possible role in the pathogenesis of ascending urinary tract infection. Am J Pathol. 1988 Oct;133(1):1–4. [PMC free article] [PubMed]
  • |ORSKOV I. Nosocomial infections with Klebsiella in lesions of the urinary tract. Acta Pathol Microbiol Scand Suppl. 1952 Jun;93:259–271. [PubMed]
  • Parkkinen J, Korhonen TK, Pere A, Hacker J, Soinila S. Binding sites in the rat brain for Escherichia coli S fimbriae associated with neonatal meningitis. J Clin Invest. 1988 Mar;81(3):860–865. [PMC free article] [PubMed]
  • Parkkinen J, Ristimäki A, Westerlund B. Binding of Escherichia coli S fimbriae to cultured human endothelial cells. Infect Immun. 1989 Jul;57(7):2256–2259. [PMC free article] [PubMed]
  • Podschun R, Heineken P, Sonntag HG. Haemagglutinins and adherence properties to HeLa and intestine 407 cells of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Mar;263(4):585–593. [PubMed]
  • Podschun R, Sievers D, Fischer A, Ullmann U. Serotypes, hemagglutinins, siderophore synthesis, and serum resistance of Klebsiella isolates causing human urinary tract infections. J Infect Dis. 1993 Dec;168(6):1415–1421. [PubMed]
  • Schurtz TA, Hornick DB, Korhonen TK, Clegg S. The type 3 fimbrial adhesin gene (mrkD) of Klebsiella species is not conserved among all fimbriate strains. Infect Immun. 1994 Oct;62(10):4186–4191. [PMC free article] [PubMed]
  • Smith SM, Digori JT, Eng RH. Epidemiology of Klebsiella antibiotic resistance and serotypes. J Clin Microbiol. 1982 Nov;16(5):868–873. [PMC free article] [PubMed]
  • Tarkkanen AM, Allen BL, Westerlund B, Holthöfer H, Kuusela P, Risteli L, Clegg S, Korhonen TK. Type V collagen as the target for type-3 fimbriae, enterobacterial adherence organelles. Mol Microbiol. 1990 Aug;4(8):1353–1361. [PubMed]
  • Tarkkanen AM, Allen BL, Williams PH, Kauppi M, Haahtela K, Siitonen A, Orskov I, Orskov F, Clegg S, Korhonen TK. Fimbriation, capsulation, and iron-scavenging systems of Klebsiella strains associated with human urinary tract infection. Infect Immun. 1992 Mar;60(3):1187–1192. [PMC free article] [PubMed]
  • Virkola R, Westerlund B, Holthöfer H, Parkkinen J, Kekomäki M, Korhonen TK. Binding characteristics of Escherichia coli adhesins in human urinary bladder. Infect Immun. 1988 Oct;56(10):2615–2622. [PMC free article] [PubMed]
  • Westerlund B, Korhonen TK. Bacterial proteins binding to the mammalian extracellular matrix. Mol Microbiol. 1993 Aug;9(4):687–694. [PubMed]

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