• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aacPermissionsJournals.ASM.orgJournalAAC ArticleJournal InfoAuthorsReviewers
Antimicrob Agents Chemother. Feb 1987; 31(2): 197–201.
PMCID: PMC174691

Comparison of histologic and quantitative techniques in evaluation of therapy for experimental Pneumocystis carinii pneumonia.

Abstract

Pneumocystis carinii pneumonia was induced in rats by the administration of corticosteroids, and histologic and quantitative techniques were compared in the evaluation of the severity of the disease and response to therapy. A highly significant correlation was found between the histologic score of the extent of alveolar involvement (the standard method of assessment) and the number of P. carinii cysts and nuclei in lung homogenates, lung weight, and lung weight/body weight ratio. Clear differences were noted between rats which responded well and rats which responded poorly to therapy by all techniques. Quantitation of P. carinii cysts and nuclei revealed a 10(4)-fold reduction in organism burden with successful treatment. Thus, these techniques should be helpful in the development of testing of new antimicrobial agents in the rat model of pneumocystosis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (922K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cushion MT, Ruffolo JJ, Linke MJ, Walzer PD. Pneumocystis carinii: growth variables and estimates in the A549 and WI-38 VA13 human cell lines. Exp Parasitol. 1985 Aug;60(1):43–54. [PubMed]
  • Cushion MT, Stanforth D, Linke MJ, Walzer PD. Method of testing the susceptibility of Pneumocystis carinii to antimicrobial agents in vitro. Antimicrob Agents Chemother. 1985 Dec;28(6):796–801. [PMC free article] [PubMed]
  • Cushion MT, Walzer PD. Growth and serial passage of Pneumocystis carinii in the A549 cell line. Infect Immun. 1984 May;44(2):245–251. [PMC free article] [PubMed]
  • Frenkel JK, Good JT, Shultz JA. Latent Pneumocystis infection of rats, relapse, and chemotherapy. Lab Invest. 1966 Oct;15(10):1559–1577. [PubMed]
  • Gay JD, Smith TF, Ilstrup DM. Comparison of processing techniques for detection of Pneumocystis carinii cysts in open-lung biopsy specimens. J Clin Microbiol. 1985 Feb;21(2):150–151. [PMC free article] [PubMed]
  • Golden JA, Sjoerdsma A, Santi DV. Pneumocystis carinii pneumonia treated with alpha-difluoromethylornithine. A prospective study among patients with the acquired immunodeficiency syndrome. West J Med. 1984 Nov;141(5):613–623. [PMC free article] [PubMed]
  • Haverkos HW. Assessment of therapy for pneumocystis carinii pneumonia. PCP Therapy Project Group. Am J Med. 1984 Mar;76(3):501–508. [PubMed]
  • Hughes WT. Limited effect of trimethoprim-sulfamethoxazole prophylaxis on Pneumocystis carinii. Antimicrob Agents Chemother. 1979 Sep;16(3):333–335. [PMC free article] [PubMed]
  • Hughes WT, Kim HK, Price RA, Miller C. Attempts at prophylaxis for murine Pneumocystis carinii pneumonitis. Curr Ther Res Clin Exp. 1973 Aug;15(8):581–587. [PubMed]
  • Hughes WT, McNabb PC, Makres TD, Feldman S. Efficacy of trimethoprim and sulfamethoxazole in the prevention and treatment of Pneumocystis carinii pneumonitis. Antimicrob Agents Chemother. 1974 Mar;5(3):289–293. [PMC free article] [PubMed]
  • Hughes WT, Smith BL. Efficacy of diaminodiphenylsulfone and other drugs in murine Pneumocystis carinii pneumonitis. Antimicrob Agents Chemother. 1984 Oct;26(4):436–440. [PMC free article] [PubMed]
  • Huppert M, Sun SH, Gleason-Jordon I, Vukovich KR. Lung weight parallels disease severity in experimental coccidioidomycosis. Infect Immun. 1976 Dec;14(6):1356–1368. [PMC free article] [PubMed]
  • Kluge RM, Spaulding DM, Spain AJ. Combination of pentamidine and trimethoprim-sulfamethoxazole in therapy of Pneumocystis carinii pneumonia in rats. Antimicrob Agents Chemother. 1978 Jun;13(6):975–978. [PMC free article] [PubMed]
  • Kovacs JA, Hiemenz JW, Macher AM, Stover D, Murray HW, Shelhamer J, Lane HC, Urmacher C, Honig C, Longo DL, et al. Pneumocystis carinii pneumonia: a comparison between patients with the acquired immunodeficiency syndrome and patients with other immunodeficiencies. Ann Intern Med. 1984 May;100(5):663–671. [PubMed]
  • Ruffolo JJ, Cushion MT, Walzer PD. Techniques for examining Pneumocystis carinii in fresh specimens. J Clin Microbiol. 1986 Jan;23(1):17–21. [PMC free article] [PubMed]
  • Thomson RB, Jr, Smith TF, Wilson WR. Comparison of two methods used to prepare smears of mouse lung tissue for detection of Pneumocystis carinii. J Clin Microbiol. 1982 Aug;16(2):303–306. [PMC free article] [PubMed]
  • Walzer PD, Perl DP, Krogstad DJ, Rawson PG, Schultz MG. Pneumocystis carinii pneumonia in the United States. Epidemiologic, diagnostic, and clinical features. Ann Intern Med. 1974 Jan;80(1):83–93. [PubMed]
  • Walzer PD, Powell RD, Jr, Yoneda K, Rutledge ME, Milder JE. Growth characteristics and pathogenesis of experimental Pneumocystis carinii pneumonia. Infect Immun. 1980 Mar;27(3):928–937. [PMC free article] [PubMed]
  • Western KA, Norman L, Kaufmann AG. Failure of pentamidine isethionate to provide chemoprophylaxis against Pneumocytis carinii infection in rats. J Infect Dis. 1975 Mar;131(3):273–276. [PubMed]
  • Wolff LJ, Baehner RL. Delayed development of pneumocystis pneumonia following administration of short-term high-dose trimethoprim-sulfamethoxazole. Am J Dis Child. 1978 May;132(5):525–526. [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...