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Sex Transm Infect. Dec 2001; 77(6): 402–408.
PMCID: PMC1744407

Mucinases and sialidases: their role in the pathogenesis of sexually transmitted infections in the female genital tract

Abstract

Background: Mucinases and sialidases contribute to the process of invasion and colonisation in many conditions and infections of the female reproductive tract by degrading the protective cervical mucus. The role of hydrolytic enzymes in the pathogenesis of sexually transmitted diseases and their effect on cervical mucus are discussed in this review.

Methods: Articles were searched for using the keywords "sialidase," "mucinase," "protease," and "sexually transmitted infections." As well as review and other articles held by our group, searches were conducted using PubMed, Grateful Med, and the University of Bath search engine, BIDS.

Results: Numerous publications were found describing the production of hydrolytic enzymes in sexually transmitted diseases. Because the number of publications exceeded the restrictions imposed on the size of the review, the authors selected and discussed those which they considered of the most relevance to sexually transmitted infections.

Key Words: mucinase; sialidase; microbial protease

Abbreviations: BSM (bovine submaxillary mucin), BV (bacterial vaginosis); Fuc (fucose); Gal (galactose); GalNAc (N-acetylgalactosamine); Glc (glucose); GlcNAc (N-acetylglucosamine); Man (mannose); PMN (polymorphonuclear neutrophils), human immunodeficiency virus 1 (HIV-1); sIgA (secretory immunoglobulin A).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • McGregor JA, French JI, Jones W, Milligan K, McKinney PJ, Patterson E, Parker R. Bacterial vaginosis is associated with prematurity and vaginal fluid mucinase and sialidase: results of a controlled trial of topical clindamycin cream. Am J Obstet Gynecol. 1994 Apr;170(4):1048–1060. [PubMed]
  • Howe L, Wiggins R, Soothill PW, Millar MR, Horner PJ, Corfield AP. Mucinase and sialidase activity of the vaginal microflora: implications for the pathogenesis of preterm labour. Int J STD AIDS. 1999 Jul;10(7):442–447. [PubMed]
  • Carlstedt I, Sheehan JK. Structure and macromolecular properties of cervical mucus glycoproteins. Symp Soc Exp Biol. 1989;43:289–316. [PubMed]
  • Eggert-Kruse W, Botz I, Pohl S, Rohr G, Strowitzki T. Antimicrobial activity of human cervical mucus. Hum Reprod. 2000 Apr;15(4):778–784. [PubMed]
  • Zopf D, Roth S. Oligosaccharide anti-infective agents. Lancet. 1996 Apr 13;347(9007):1017–1021. [PubMed]
  • Enhörning G, Huldt L, Melén B. Ability of cervical mucus to act as a barrier against bacteria. Am J Obstet Gynecol. 1970 Oct 15;108(4):532–537. [PubMed]
  • Elstein M. Functions and physical properties of mucus in the female genital tract. Br Med Bull. 1978 Jan;34(1):83–88. [PubMed]
  • Scudder PR, Chantler EN. Control of human cervical mucin glycosylation by endogenous fucosyl and sialyltransferases. Adv Exp Med Biol. 1982;144:265–267. [PubMed]
  • Corfield T. Bacterial sialidases--roles in pathogenicity and nutrition. Glycobiology. 1992 Dec;2(6):509–521. [PubMed]
  • Wickström C, Davies JR, Eriksen GV, Veerman EC, Carlstedt I. MUC5B is a major gel-forming, oligomeric mucin from human salivary gland, respiratory tract and endocervix: identification of glycoforms and C-terminal cleavage. Biochem J. 1998 Sep 15;334(Pt 3):685–693. [PMC free article] [PubMed]
  • Gipson IK, Spurr-Michaud S, Moccia R, Zhan Q, Toribara N, Ho SB, Gargiulo AR, Hill JA., 3rd MUC4 and MUC5B transcripts are the prevalent mucin messenger ribonucleic acids of the human endocervix. Biol Reprod. 1999 Jan;60(1):58–64. [PubMed]
  • Audie JP, Tetaert D, Pigny P, Buisine MP, Janin A, Aubert JP, Porchet N, Boersma A. Mucin gene expression in the human endocervix. Hum Reprod. 1995 Jan;10(1):98–102. [PubMed]
  • Gipson IK, Moccia R, Spurr-Michaud S, Argüeso P, Gargiulo AR, Hill JA, 3rd, Offner GD, Keutmann HT. The Amount of MUC5B mucin in cervical mucus peaks at midcycle. J Clin Endocrinol Metab. 2001 Feb;86(2):594–600. [PubMed]
  • Bagriaçik EU, Miller KS. Cell surface sialic acid and the regulation of immune cell interactions: the neuraminidase effect reconsidered. Glycobiology. 1999 Mar;9(3):267–275. [PubMed]
  • Bardsley-Elliot A, Noble S. Oseltamivir. Drugs. 1999 Nov;58(5):851–862. [PubMed]
  • Kilian M, Reinholdt J, Lomholt H, Poulsen K, Frandsen EV. Biological significance of IgA1 proteases in bacterial colonization and pathogenesis: critical evaluation of experimental evidence. APMIS. 1996 May;104(5):321–338. [PubMed]
  • Alugupalli KR, Kalfas S. Degradation of lactoferrin by periodontitis-associated bacteria. FEMS Microbiol Lett. 1996 Dec 1;145(2):209–214. [PubMed]
  • Stewart-Tull DE, Ollar RA, Scobie TS. Studies on the Vibrio cholerae mucinase complex. I. Enzymic activities associated with the complex. J Med Microbiol. 1986 Dec;22(4):325–333. [PubMed]
  • Dwarakanath AD, Campbell BJ, Tsai HH, Sunderland D, Hart CA, Rhodes JM. Faecal mucinase activity assessed in inflammatory bowel disease using 14C threonine labelled mucin substrate. Gut. 1995 Jul;37(1):58–62. [PMC free article] [PubMed]
  • Connaris S, Greenwell P. Glycosidases in mucin-dwelling protozoans. Glycoconj J. 1997 Nov;14(7):879–882. [PubMed]
  • Macfarlane GT, Hay S, Gibson GR. Influence of mucin on glycosidase, protease and arylamidase activities of human gut bacteria grown in a 3-stage continuous culture system. J Appl Bacteriol. 1989 May;66(5):407–417. [PubMed]
  • Lehker MW, Sweeney D. Trichomonad invasion of the mucous layer requires adhesins, mucinases, and motility. Sex Transm Infect. 1999 Aug;75(4):231–238. [PMC free article] [PubMed]
  • de Repentigny L, Aumont F, Bernard K, Belhumeur P. Characterization of binding of Candida albicans to small intestinal mucin and its role in adherence to mucosal epithelial cells. Infect Immun. 2000 Jun;68(6):3172–3179. [PMC free article] [PubMed]
  • Macfarlane GT, Gibson GR. Formation of glycoprotein degrading enzymes by Bacteroides fragilis. FEMS Microbiol Lett. 1991 Jan 15;61(2-3):289–293. [PubMed]
  • Robertson AM, Wright DP. Bacterial glycosulphatases and sulphomucin degradation. Can J Gastroenterol. 1997 May-Jun;11(4):361–366. [PubMed]
  • Nieuw Amerongen AV, Bolscher JG, Bloemena E, Veerman EC. Sulfomucins in the human body. Biol Chem. 1998 Jan;379(1):1–18. [PubMed]
  • Drzeniek R. Viral and bacterial neuraminidases. Curr Top Microbiol Immunol. 1972;59:35–74. [PubMed]
  • Vimr ER. Microbial sialidases: does bigger always mean better? Trends Microbiol. 1994 Aug;2(8):271–277. [PubMed]
  • Kelm S, Schauer R. Sialic acids in molecular and cellular interactions. Int Rev Cytol. 1997;175:137–240. [PubMed]
  • Kirjavainen PV, Ouwehand AC, Isolauri E, Salminen SJ. The ability of probiotic bacteria to bind to human intestinal mucus. FEMS Microbiol Lett. 1998 Oct 15;167(2):185–189. [PubMed]
  • Herold BC, Siston A, Bremer J, Kirkpatrick R, Wilbanks G, Fugedi P, Peto C, Cooper M. Sulfated carbohydrate compounds prevent microbial adherence by sexually transmitted disease pathogens. Antimicrob Agents Chemother. 1997 Dec;41(12):2776–2780. [PMC free article] [PubMed]
  • Briselden AM, Moncla BJ, Stevens CE, Hillier SL. Sialidases (neuraminidases) in bacterial vaginosis and bacterial vaginosis-associated microflora. J Clin Microbiol. 1992 Mar;30(3):663–666. [PMC free article] [PubMed]
  • Puapermpoonsiri S, Kato N, Watanabe K, Ueno K, Chongsomchai C, Lumbiganon P. Vaginal microflora associated with bacterial vaginosis in Japanese and Thai pregnant women. Clin Infect Dis. 1996 Oct;23(4):748–752. [PubMed]
  • Cauci S, Driussi S, Monte R, Lanzafame P, Pitzus E, Quadrifoglio F. Immunoglobulin A response against Gardnerella vaginalis hemolysin and sialidase activity in bacterial vaginosis. Am J Obstet Gynecol. 1998 Mar;178(3):511–515. [PubMed]
  • Wiggins R, Crowley T, Horner PJ, Soothill PW, Millar MR, Corfield AP. Use of 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid in a novel spot test To identify sialidase activity in vaginal swabs from women with bacterial vaginosis. J Clin Microbiol. 2000 Aug;38(8):3096–3097. [PMC free article] [PubMed]
  • Costerton JW. Overview of microbial biofilms. J Ind Microbiol. 1995 Sep;15(3):137–140. [PubMed]
  • Godoy VG, Dallas MM, Russo TA, Malamy MH. A role for Bacteroides fragilis neuraminidase in bacterial growth in two model systems. Infect Immun. 1993 Oct;61(10):4415–4426. [PMC free article] [PubMed]
  • Guzmán CA, Platé M, Pruzzo C. Role of neuraminidase-dependent adherence in Bacteroides fragilis attachment to human epithelial cells. FEMS Microbiol Lett. 1990 Sep 1;59(1-2):187–192. [PubMed]
  • Namavar F, Van der Bijl MW, Appelmelk BJ, De Graaff J, MacLaren DM. The role of neuraminidase in haemagglutination and adherence to colon WiDr cells by Bacteroides fragilis. J Med Microbiol. 1994 Jun;40(6):393–396. [PubMed]
  • Domingues RM, Cavalcanti SM, Andrade AF, Ferreira MC. Sialic acid as receptor of Bacteroides fragilis lectin-like adhesin. Zentralbl Bakteriol. 1992 Oct;277(3):340–344. [PubMed]
  • Berg JO, Lindqvist L, Andersson G, Nord CE. Neuraminidase in Bacteroides fragilis. Appl Environ Microbiol. 1983 Jul;46(1):75–80. [PMC free article] [PubMed]
  • Gibson SA, Macfarlane GT. Characterization of proteases formed by Bacteroides fragilis. J Gen Microbiol. 1988 Aug;134(8):2231–2240. [PubMed]
  • Moncla BJ, Braham P, Hillier SL. Sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. J Clin Microbiol. 1990 Mar;28(3):422–425. [PMC free article] [PubMed]
  • Gilks CB, Reid PE, Clement PB, Owen DA. Histochemical changes in cervical mucus-secreting epithelium during the normal menstrual cycle. Fertil Steril. 1989 Feb;51(2):286–291. [PubMed]
  • Kahane I, Reisch-Saada A, Almagor M, Abeliuck P, Yatziv S. Glycosidase activities of mycoplasmas. Zentralbl Bakteriol. 1990 Aug;273(3):300–305. [PubMed]
  • Robertson JA, Stemler ME, Stemke GW. Immunoglobulin A protease activity of Ureaplasma urealyticum. J Clin Microbiol. 1984 Feb;19(2):255–258. [PMC free article] [PubMed]
  • Smith DG, Russell WC, Thirkell D. Adherence of Ureaplasma urealyticum to human epithelial cells. Microbiology. 1994 Oct;140(Pt 10):2893–2898. [PubMed]
  • Vetere A, Borriello SP, Fontaine E, Reed PJ, Taylor-Robinson D. Characterisation of anaerobic curved rods (Mobiluncus spp.) isolated from the urogenital tract. J Med Microbiol. 1987 May;23(3):279–288. [PubMed]
  • Murray PA, Kern DG, Winkler JR. Identification of a galactose-binding lectin on Fusobacterium nucleatum FN-2. Infect Immun. 1988 May;56(5):1314–1319. [PMC free article] [PubMed]
  • Mangan DF, Novak MJ, Vora SA, Mourad J, Kriger PS. Lectinlike interactions of Fusobacterium nucleatum with human neutrophils. Infect Immun. 1989 Nov;57(11):3601–3611. [PMC free article] [PubMed]
  • Ruseler-van Embden JG, van Lieshout LM. Increased faecal glycosidases in patients with Crohn's disease. Digestion. 1987;37(1):43–50. [PubMed]
  • Coombs GH, North MJ. An analysis of the proteinases of Trichomonas vaginalis by polyacrylamide gel electrophoresis. Parasitology. 1983 Feb;86(Pt 1):1–6. [PubMed]
  • Garber GE, Lemchuk-Favel LT. Analysis of the extracellular proteases of Trichomonas vaginalis. Parasitol Res. 1994;80(5):361–365. [PubMed]
  • Bózner P, Demes P. Proteinases in Trichomonas vaginalis and Tritrichomonas mobilensis are not exclusively of cysteine type. Parasitology. 1991 Feb;102(Pt 1):113–115. [PubMed]
  • Padilla-Vaca F, Anaya-Velázquez F. Biochemical properties of a neuraminidase of Trichomonas vaginalis. J Parasitol. 1997 Dec;83(6):1001–1006. [PubMed]
  • Yolken RH. Enzymic analysis for rapid detection of microbial infection in human body fluids: an overview. Clin Chem. 1981 Sep;27(9):1490–1498. [PubMed]
  • Wellmer A. Adhärenz von klinischen Candida-albicans-Isolaten an bukkalen Epithelzellen. Mycoses. 1999;42 (Suppl 1):43–47. [PubMed]
  • Hedges SR, Mayo MS, Kallman L, Mestecky J, Hook EW, 3rd, Russell MW. Evaluation of immunoglobulin A1 (IgA1) protease and IgA1 protease-inhibitory activity in human female genital infection with Neisseria gonorrhoeae. Infect Immun. 1998 Dec;66(12):5826–5832. [PMC free article] [PubMed]
  • Pritchard DG, Lin B. Group B streptococcal neuraminidase is actually a hyaluronidase. Infect Immun. 1993 Aug;61(8):3234–3239. [PMC free article] [PubMed]
  • Swanson AF, Kuo CC. Identification of lectin-binding proteins in Chlamydia species. Infect Immun. 1990 Feb;58(2):502–507. [PMC free article] [PubMed]

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