• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Jan 1996; 64(1): 209–214.
PMCID: PMC173747

Vitamin A deficiency predisposes to Staphylococcus aureus infection.

Abstract

We have investigated the consequences of vitamin A deficiency in a rat model of T-cell-dependent and superantigen-mediated Staphylococcus aureus arthritis. After intravenous inoculation of enterotoxin A-producing staphylococci, the vitamin-A-deficient rats showed a decreased weight gain compared with the paired fed controls despite equal food consumption. The control rats developed arthritis in the first few days after bacterial inoculation, with a peak frequency at day 5, and then gradually recovered; however, the frequency of arthritis 18 days after bacterial inoculation was 86% among the vitamin A-deficient rats and 44% among the control rats. During this period, 3 of 10 deficient rats and 1 of 10 control rats died. Further in vitro analysis revealed that T-cell responses to S. aureus were significantly higher in the vitamin A-deficient rats than in the control animals. In contrast, B-cell reactivity, measured as immunoglobulin levels, autoantibody levels, and specific antibacterial antibody levels in serum, did not differ between the groups. Interestingly, the innate host defense mechanisms against S. aureus were also profoundly affected by vitamin A deficiency. Thus, despite a larger number of circulating phagocytic cells in the vitamin-A-deficient group, the capacity to phagocytize and exert intracellular killing of S. aureus was significantly decreased in comparison with the control rats. Furthermore, serum from the vitamin A-deficient rats inoculated with Staphylococcus aureus displayed decreased complement lysis activity. Our results suggest that the increased susceptibility to S. aureus infection observed in the vitamin-A-deficient rats is due to a concerted action of antigen-specific T-cell hyperactivity, impaired function of the phagocytes, and decreased complement activity.

Full Text

The Full Text of this article is available as a PDF (234K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aarden LA, De Groot ER, Schaap OL, Lansdorp PM. Production of hybridoma growth factor by human monocytes. Eur J Immunol. 1987 Oct;17(10):1411–1416. [PubMed]
  • Abdelnour A, Bremell T, Holmdahl R, Tarkowski A. Clonal expansion of T lymphocytes causes arthritis and mortality in mice infected with toxic shock syndrome toxin-1-producing staphylococci. Eur J Immunol. 1994 May;24(5):1161–1166. [PubMed]
  • Abdelnour A, Bremell T, Holmdahl R, Tarkowski A. Role of T lymphocytes in experimental Staphylococcus aureus arthritis. Scand J Immunol. 1994 Apr;39(4):403–408. [PubMed]
  • Arthur P, Kirkwood B, Ross D, Morris S, Gyapong J, Tomkins A, Addy H. Impact of vitamin A supplementation on childhood morbidity in northern Ghana. Lancet. 1992 Feb 8;339(8789):361–362. [PubMed]
  • Bellinati-Pires R, Carneiro-Sampaio MM, Colletto GM. Functional evaluation of human neutrophils. Is the bactericidal activity correlated with nitroblue tetrazolium reduction? J Investig Allergol Clin Immunol. 1992 May-Jun;2(3):146–153. [PubMed]
  • Bremell T, Holmdahl R, Tarkowski A. Protective role of sialophorin (CD43)-expressing cells in experimental Staphylococcus aureus infection. Infect Immun. 1994 Oct;62(10):4637–4640. [PMC free article] [PubMed]
  • Bremell T, Lange S, Holmdahl R, Rydén C, Hansson GK, Tarkowski A. Immunopathological features of rat Staphylococcus aureus arthritis. Infect Immun. 1994 Jun;62(6):2334–2344. [PMC free article] [PubMed]
  • Carman JA, Hayes CE. Abnormal regulation of IFN-gamma secretion in vitamin A deficiency. J Immunol. 1991 Aug 15;147(4):1247–1252. [PubMed]
  • Catignani GL, Bieri JG. Simultaneous determination of retinol and alpha-tocopherol in serum or plasma by liquid chromatography. Clin Chem. 1983 Apr;29(4):708–712. [PubMed]
  • Dahlgren U, Ahlstedt S, Andersson T, Hedman L, Hanson LA. IgA antibodies in rat bile are not solely derived from thoracic duct lymph. Scand J Immunol. 1983 Jun;17(6):569–574. [PubMed]
  • Davis CY, Sell JL. Immunoglobulin concentrations in serum and tissues of vitamin A-deficient broiler chicks after Newcastle disease virus vaccination. Poult Sci. 1989 Jan;68(1):136–144. [PubMed]
  • Elwing H, Nygren H. Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): a simple method for quantitation of class-specific antibodies. J Immunol Methods. 1979;31(1-2):101–107. [PubMed]
  • Friedman A, Sklan D. Antigen-specific immune response impairment in the chick as influenced by dietary vitamin A. J Nutr. 1989 May;119(5):790–795. [PubMed]
  • Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed]
  • Goldenberg DL, Reed JI. Bacterial arthritis. N Engl J Med. 1985 Mar 21;312(12):764–771. [PubMed]
  • Goldenberg DL, Chisholm PL, Rice PA. Experimental models of bacterial arthritis: a microbiologic and histopathologic characterization of the arthritis after the intraarticular injections of Neisseria gonorrhoeae, Staphylococcus aureus, group A streptococci, and Escherichia coli. J Rheumatol. 1983 Feb;10(1):5–11. [PubMed]
  • Helle M, Boeije L, Aarden LA. Functional discrimination between interleukin 6 and interleukin 1. Eur J Immunol. 1988 Oct;18(10):1535–1540. [PubMed]
  • Lissner CR, Swanson RN, O'Brien AD. Genetic control of the innate resistance of mice to Salmonella typhimurium: expression of the Ity gene in peritoneal and splenic macrophages isolated in vitro. J Immunol. 1983 Dec;131(6):3006–3013. [PubMed]
  • Madjid B, Sirisinha S, Lamb AJ. The effect of vitamin A and protein deficiency on complement levels in rats. Proc Soc Exp Biol Med. 1978 May;158(1):92–95. [PubMed]
  • Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. [PubMed]
  • Mikhail IS, Alarcón GS. Nongonococcal bacterial arthritis. Rheum Dis Clin North Am. 1993 May;19(2):311–331. [PubMed]
  • Nauss KM, Mark DA, Suskind RM. The effect of vitamin A deficiency on the in vitro cellular immune response of rats. J Nutr. 1979 Oct;109(10):1815–1823. [PubMed]
  • Ongsakul M, Sirisinha S, Lamb AJ. Impaired blood clearance of bacteria and phagocytic activity in vitamin A-deficient rats. Proc Soc Exp Biol Med. 1985 Feb;178(2):204–208. [PubMed]
  • Pasatiempo AM, Kinoshita M, Taylor CE, Ross AC. Antibody production in vitamin A-depleted rats is impaired after immunization with bacterial polysaccharide or protein antigens. FASEB J. 1990 May;4(8):2518–2527. [PubMed]
  • Rahmathullah L, Underwood BA, Thulasiraj RD, Milton RC. Diarrhea, respiratory infections, and growth are not affected by a weekly low-dose vitamin A supplement: a masked, controlled field trial in children in southern India. Am J Clin Nutr. 1991 Sep;54(3):568–577. [PubMed]
  • Sijtsma SR, Rombout JH, Dohmen MJ, West CE, van der Zijpp AJ. Effect of vitamin A deficiency on the activity of macrophages in Newcastle disease virus-infected chickens. Vet Immunol Immunopathol. 1991 Mar;28(1):17–27. [PubMed]
  • Sijtsma SR, Rombout JH, Kiepurski A, West CE, van der Zijpp AJ. Changes in lymphoid organs and blood lymphocytes induced by vitamin A deficiency and Newcastle disease virus infection in chickens. Dev Comp Immunol. 1991 Fall;15(4):349–356. [PubMed]
  • Sommer A, Tarwotjo I, Djunaedi E, West KP, Jr, Loeden AA, Tilden R, Mele L. Impact of vitamin A supplementation on childhood mortality. A randomised controlled community trial. Lancet. 1986 May 24;1(8491):1169–1173. [PubMed]
  • Tarkowski A, Czerkinsky C, Nilsson LA. Detection of IgG rheumatoid factor secreting cells in autoimmune MRL/1 mice: a kinetic study. Clin Exp Immunol. 1984 Oct;58(1):7–12. [PMC free article] [PubMed]
  • West KP, Jr, Howard GR, Sommer A. Vitamin A and infection: public health implications. Annu Rev Nutr. 1989;9:63–86. [PubMed]
  • Wiedermann U, Hanson LA, Bremell T, Kahu H, Dahlgren UI. Increased translocation of Escherichia coli and development of arthritis in vitamin A-deficient rats. Infect Immun. 1995 Aug;63(8):3062–3068. [PMC free article] [PubMed]
  • Wiedermann U, Hanson LA, Holmgren J, Kahu H, Dahlgren UI. Impaired mucosal antibody response to cholera toxin in vitamin A-deficient rats immunized with oral cholera vaccine. Infect Immun. 1993 Sep;61(9):3952–3957. [PMC free article] [PubMed]
  • Wiedermann U, Hanson LA, Kahu H, Dahlgren UI. Aberrant T-cell function in vitro and impaired T-cell dependent antibody response in vivo in vitamin A-deficient rats. Immunology. 1993 Dec;80(4):581–586. [PMC free article] [PubMed]
  • Zhao YX, Abdelnour A, Holmdahl R, Tarkowski A. Mice with the xid B cell defect are less susceptible to developing Staphylococcus aureus-induced arthritis. J Immunol. 1995 Aug 15;155(4):2067–2076. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...