• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Dec 1995; 63(12): 4669–4674.
PMCID: PMC173670

FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis.

Abstract

Adherence of microorganisms to damaged heart tissue is a crucial event in the pathogenesis of infective endocarditis. In the present study, we investigated the role of the FimA protein as a potential virulence factor associated with Streptococcus parasanguis endocarditis. FimA is a 36-kDa surface protein that is a recognized adhesin in the oral cavity where it mediates adherence to the salivary pellicle. An insertion mutant and a deletion mutant of S. parasanguis were employed in the rat model of endocarditis to determine the relevance of FimA in endocarditis pathogenesis. Catheterized rats were infected with either the fimA deletion mutant VT929, the fimA insertion mutant VT930, or the isogenic, wild-type S. parasanguis FW213. Rats inoculated with FW213 developed endocarditis more frequently (50.9%) than animals inoculated with either the deletion mutant (2.7%) or the insertion mutant (7.6%) (P < 0.001). A series of in vitro assays were performed to explore the mechanism(s) by which FimA enhanced the infectivity of S. parasanguis. FimA did not inhibit the uptake or the subsequent killing of S. parasanguis by phagocytic granulocytes. Similarly, FimA did not play a role in the adherence to or the aggregation of platelets. Significant differences were noted between FW213 and VT929 (P < 0.05) and FW213 and VT930 (P < 0.001) in their abilities to bind to fibrin monolayers. The mean percent adherence of FW213 to fibrin monolayers (2.1%) was greater than those of VT929 (0.5%) and VT930 (0.12%). Taken together, these results indicate that FimA is a major virulence determinant associated with S. parasanguis endocarditis and further suggest that its role is associated with initial colonization of damaged heart tissue.

Full Text

The Full Text of this article is available as a PDF (211K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Dankert J, van der Werff J, Zaat SA, Joldersma W, Klein D, Hess J. Involvement of bactericidal factors from thrombin-stimulated platelets in clearance of adherent viridans streptococci in experimental infective endocarditis. Infect Immun. 1995 Feb;63(2):663–671. [PMC free article] [PubMed]
  • Douglas CW, Brown PR, Preston FE. Platelet aggregation by oral streptococci. FEMS Microbiol Lett. 1990 Oct;60(1-2):63–67. [PubMed]
  • Facklam RR. Physiological differentiation of viridans streptococci. J Clin Microbiol. 1977 Feb;5(2):184–201. [PMC free article] [PubMed]
  • Fenno JC, LeBlanc DJ, Fives-Taylor P. Nucleotide sequence analysis of a type 1 fimbrial gene of Streptococcus sanguis FW213. Infect Immun. 1989 Nov;57(11):3527–3533. [PMC free article] [PubMed]
  • Fenno JC, Shaikh A, Fives-Taylor P. Characterization of allelic replacement in Streptococcus parasanguis: transformation and homologous recombination in a 'nontransformable' streptococcus. Gene. 1993 Aug 16;130(1):81–90. [PubMed]
  • Fenno JC, Shaikh A, Spatafora G, Fives-Taylor P. The fimA locus of Streptococcus parasanguis encodes an ATP-binding membrane transport system. Mol Microbiol. 1995 Mar;15(5):849–863. [PubMed]
  • Ganeshkumar N, Hannam PM, Kolenbrander PE, McBride BC. Nucleotide sequence of a gene coding for a saliva-binding protein (SsaB) from Streptococcus sanguis 12 and possible role of the protein in coaggregation with actinomyces. Infect Immun. 1991 Mar;59(3):1093–1099. [PMC free article] [PubMed]
  • Herzberg MC, Brintzenhofe KL, Clawson CC. Aggregation of human platelets and adhesion of Streptococcus sanguis. Infect Immun. 1983 Mar;39(3):1457–1469. [PMC free article] [PubMed]
  • Herzberg MC, Gong K, MacFarlane GD, Erickson PR, Soberay AH, Krebsbach PH, Manjula G, Schilling K, Bowen WH. Phenotypic characterization of Streptococcus sanguis virulence factors associated with bacterial endocarditis. Infect Immun. 1990 Feb;58(2):515–522. [PMC free article] [PubMed]
  • Herzberg MC, MacFarlane GD, Gong K, Armstrong NN, Witt AR, Erickson PR, Meyer MW. The platelet interactivity phenotype of Streptococcus sanguis influences the course of experimental endocarditis. Infect Immun. 1992 Nov;60(11):4809–4818. [PMC free article] [PubMed]
  • Johnson CM. Adherence events in the pathogenesis of infective endocarditis. Infect Dis Clin North Am. 1993 Mar;7(1):21–36. [PubMed]
  • Kolenbrander PE, Andersen RN, Ganeshkumar N. Nucleotide sequence of the Streptococcus gordonii PK488 coaggregation adhesin gene, scaA, and ATP-binding cassette. Infect Immun. 1994 Oct;62(10):4469–4480. [PMC free article] [PubMed]
  • Lowe AM, Lambert PA, Smith AW. Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci. Infect Immun. 1995 Feb;63(2):703–706. [PMC free article] [PubMed]
  • Lowrance JH, Baddour LM, Simpson WA. The role of fibronectin binding in the rat model of experimental endocarditis caused by Streptococcus sanguis. J Clin Invest. 1990 Jul;86(1):7–13. [PMC free article] [PubMed]
  • Munro CL, Macrina FL. Sucrose-derived exopolysaccharides of Streptococcus mutans V403 contribute to infectivity in endocarditis. Mol Microbiol. 1993 Apr;8(1):133–142. [PubMed]
  • Oligino L, Fives-Taylor P. Overexpression and purification of a fimbria-associated adhesin of Streptococcus parasanguis. Infect Immun. 1993 Mar;61(3):1016–1022. [PMC free article] [PubMed]
  • Sampson JS, O'Connor SP, Stinson AR, Tharpe JA, Russell H. Cloning and nucleotide sequence analysis of psaA, the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins. Infect Immun. 1994 Jan;62(1):319–324. [PMC free article] [PubMed]
  • Scheld WM, Strunk RW, Balian G, Calderone RA. Microbial adhesion to fibronectin in vitro correlates with production of endocarditis in rabbits. Proc Soc Exp Biol Med. 1985 Dec;180(3):474–482. [PubMed]
  • Scheld WM, Valone JA, Sande MA. Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin. J Clin Invest. 1978 May;61(5):1394–1404. [PMC free article] [PubMed]
  • Sommer P, Gleyzal C, Guerret S, Etienne J, Grimaud JA. Induction of a putative laminin-binding protein of Streptococcus gordonii in human infective endocarditis. Infect Immun. 1992 Feb;60(2):360–365. [PMC free article] [PubMed]
  • Switalski LM, Murchison H, Timpl R, Curtiss R, 3rd, Hök M. Binding of laminin to oral and endocarditis strains of viridans streptococci. J Bacteriol. 1987 Mar;169(3):1095–1101. [PMC free article] [PubMed]
  • Trieu-Cuot P, Courvalin P. Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III. Gene. 1983 Sep;23(3):331–341. [PubMed]
  • Vercellotti GM, Lussenhop D, Peterson PK, Furcht LT, McCarthy JB, Jacob HS, Moldow CF. Bacterial adherence to fibronectin and endothelial cells: a possible mechanism for bacterial tissue tropism. J Lab Clin Med. 1984 Jan;103(1):34–43. [PubMed]
  • Wu T, Yeaman MR, Bayer AS. In vitro resistance to platelet microbicidal protein correlates with endocarditis source among bacteremic staphylococcal and streptococcal isolates. Antimicrob Agents Chemother. 1994 Apr;38(4):729–732. [PMC free article] [PubMed]
  • Yeaman MR, Ibrahim AS, Edwards JE, Jr, Bayer AS, Ghannoum MA. Thrombin-induced rabbit platelet microbicidal protein is fungicidal in vitro. Antimicrob Agents Chemother. 1993 Mar;37(3):546–553. [PMC free article] [PubMed]
  • Yeaman MR, Norman DC, Bayer AS. Staphylococcus aureus susceptibility to thrombin-induced platelet microbicidal protein is independent of platelet adherence and aggregation in vitro. Infect Immun. 1992 Jun;60(6):2368–2374. [PMC free article] [PubMed]
  • Yeaman MR, Puentes SM, Norman DC, Bayer AS. Partial characterization and staphylocidal activity of thrombin-induced platelet microbicidal protein. Infect Immun. 1992 Mar;60(3):1202–1209. [PMC free article] [PubMed]
  • Yeaman MR, Sullam PM, Dazin PF, Bayer AS. Platelet microbicidal protein alone and in combination with antibiotics reduces Staphylococcus aureus adherence to platelets in vitro. Infect Immun. 1994 Aug;62(8):3416–3423. [PMC free article] [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...